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Nthl1 (NM_008743) Mouse Tagged ORF Clone
CAT＃: MG218943
- TrueORF®
Nthl1 (tGFP-tagged) - Mouse nth (endonuclease III)-like 1 (E.coli) (Nthl1), (10ug)
ORF Plasmid: DDK
"NM_008743" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Nth1; Octs3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218943 representing NM_008743
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCAGGGGTGCGGATGGTGACTCGCAGTCGGAGCCGCGCGACTAGGATCGCGTCGGAAGGGTGTA GGGAGGAGCTCGCCCCGCGAGAGGCTGCTGCAGAAGGAAGAAAAAGCCACAGGCCCGTGAGACATCCACG GAGAACACAGAAAACGCATGTGGCCTATGAAGCGGCTAATGGTGAGGAAGGCGAAGATGCTGAGCCCCTC AAAGTGCCCGTTTGGGAGCCCCAGAACTGGCAGCAGCAACTGGCCAACATCCGCATCATGAGAAGCAAGA AGGATGCACCTGTGGACCAGCTAGGCGCCGAGCACTGCTATGATGCAAGTGCCCCCCCGAAGGTGAGGAG GTACCAGGTACTCCTGTCGCTGATGCTCTCCAGCCAGACCAAAGACCAGGTCACAGCAGGTGCCATGCAA CGGCTCCGGGCCCGGGGCTTGACTGTGGAGAGCATCCTGCAGACCGATGATGACACGCTAGGCAGACTCA TCTACCCTGTGGGCTTCTGGAGGAACAAGGTAAAATACATCAAGCAGACAACCGCCATCCTGCAGCAGCG CTACGAAGGGGACATCCCTGCTTCCGTGGCTGAGCTGGTAGCCTTGCCAGGTGTTGGGCCCAAGATGGCA CACTTGGCTATGGCTGTGGCCTGGGGGACCATATCAGGCATAGCAGTGGACACACATGTGCACAGAATAG CCAACAGACTGAGGTGGACCAAGAAGATGACCAAGACCCCAGAAGAGACACGCAAGAACTTGGAAGAGTG GCTACCCAGGGTGCTGTGGAGTGAGGTCAACGGACTACTGGTAGGCTTCGGCCAACAGATCTGTCTTCCT GTCCATCCTCGATGTCAGGCTTGCCTCAACAAGGCCCTGTGCCCTGCTGCCCAGGATCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218943 representing NM_008743
Red=Cloning site Green=Tags(s) MNSGVRMVTRSRSRATRIASEGCREELAPREAAAEGRKSHRPVRHPRRTQKTHVAYEAANGEEGEDAEPL KVPVWEPQNWQQQLANIRIMRSKKDAPVDQLGAEHCYDASAPPKVRRYQVLLSLMLSSQTKDQVTAGAMQ RLRARGLTVESILQTDDDTLGRLIYPVGFWRNKVKYIKQTTAILQQRYEGDIPASVAELVALPGVGPKMA HLAMAVAWGTISGIAVDTHVHRIANRLRWTKKMTKTPEETRKNLEEWLPRVLWSEVNGLLVGFGQQICLP VHPRCQACLNKALCPAAQDL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008743 |
| ORF Size | 900 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008743.2, NP_032769.2 |
| RefSeq Size | 1080 bp |
| RefSeq ORF | 903 bp |
| Locus ID | 18207 |
| UniProt ID | O35980 |
| Gene Summary | Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209061 | Nthl1 (untagged) - Mouse nth (endonuclease III)-like 1 (E.coli) (Nthl1), (10ug) |
CNY 3,990.00 |
|
| MR218943 | Nthl1 (Myc-DDK-tagged) - Mouse nth (endonuclease III)-like 1 (E.coli) (Nthl1) |
CNY 2,400.00 |
|
| MR218943L3 | Lenti ORF clone of Nthl1 (Myc-DDK-tagged) - Mouse nth (endonuclease III)-like 1 (E.coli) (Nthl1) |
CNY 4,750.00 |
|
| MR218943L4 | Lenti ORF clone of Nthl1 (mGFP-tagged) - Mouse nth (endonuclease III)-like 1 (E.coli) (Nthl1) |
CNY 4,750.00 |
