Cyb5r2 (NM_177216) Mouse Tagged ORF Clone
CAT#: MG218956
- TrueORF®
Cyb5r2 (tGFP-tagged) - Mouse cytochrome b5 reductase 2 (Cyb5r2), (10ug)
ORF Plasmid: DDK
"NM_177216" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | b5R.2; D630003K02Rik |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG218956 representing NM_177216
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGTCAAGAAGAAAGATCTTATCACCTTACAGGACCCTGAAGCCAAGTACCCGCTGCCCTTGATTG AGAAGGAGCAAATCAGTCACAACACCAGAAGGTTCCGCTTTGGGCTGCCCTCACCAGACCATGTCCTGGG GCTTCCTGTCGGTAACTATGTCCATCTCTTGGCACAAATTAACAATGAATTGGTGATCAGGGCTTACACA CCTGTCTCCAGTGACGATGATCAAGGCTTTGTGGACTTAATTATAAAGATCTACTTCAAAAATGTGCACC CCAAGTATCCTGAAGGAGGAAAGATGACTCAGTACTTGGAGAACATGAAAATTGGGGATACCATCCTTTT TCGGGGGCCAACAGGGCGCCTGTTCTACAATGAGCCAGGAACCATTTTAATCAAAGCAAACAAAACAAGT GAGCCTGAAAAAAAGCTGGTTCACCACCTGGGGATGATTGCTGGAGGCACAGGCATTACGCCCATGCTAC AGCTGATTCGCCACATCACCAAGGACACCAGCGATGAGACCAGGATGTCCCTCCTCTTTGCTAACCAGAC AGAAGAGGATATCTTGCTGAGAAAGGAGCTAGAAGAAGTCGCCACGACTCATCACAAACAGTTCAACCTG TGGTATACCCTGGACAGGCCTCCCAGTGACTGGAAGTACAGCTCAGGCTTTGTTAGCGCTGACATGATCA AGGAGCACCTCCCTCCTCCTGGGGAGGACACGCTCATCCTGGTGTGTGGCCCACCACCTCTGATCCAGGC TGCCGCTCACCCCAGCCTGGAGCAGCTCGGCTATACCAAGGACATGATTTTTACCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218956 representing NM_177216
Red=Cloning site Green=Tags(s) MSVKKKDLITLQDPEAKYPLPLIEKEQISHNTRRFRFGLPSPDHVLGLPVGNYVHLLAQINNELVIRAYT PVSSDDDQGFVDLIIKIYFKNVHPKYPEGGKMTQYLENMKIGDTILFRGPTGRLFYNEPGTILIKANKTS EPEKKLVHHLGMIAGGTGITPMLQLIRHITKDTSDETRMSLLFANQTEEDILLRKELEEVATTHHKQFNL WYTLDRPPSDWKYSSGFVSADMIKEHLPPPGEDTLILVCGPPPLIQAAAHPSLEQLGYTKDMIFTY TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_177216 |
ORF Size | 828 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177216.3, NP_796190.1 |
RefSeq Size | 2688 bp |
RefSeq ORF | 831 bp |
Locus ID | 320635 |
UniProt ID | Q3KNK3 |
Gene Summary | NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC214476 | Cyb5r2 (untagged) - Mouse cytochrome b5 reductase 2 (Cyb5r2), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
MR218956 | Cyb5r2 (Myc-DDK-tagged) - Mouse cytochrome b5 reductase 2 (Cyb5r2), transcript variant 2 |
CNY 2,400.00 |
|
MR218956L3 | Lenti ORF clone of Cyb5r2 (Myc-DDK-tagged) - Mouse cytochrome b5 reductase 2 (Cyb5r2), transcript variant 2 |
CNY 4,750.00 |
|
MR218956L4 | Lenti ORF clone of Cyb5r2 (mGFP-tagged) - Mouse cytochrome b5 reductase 2 (Cyb5r2), transcript variant 2 |
CNY 4,750.00 |