Brk1 (NM_133937) Mouse Tagged ORF Clone
CAT#: MG219863
- TrueORF®
Brk1 (tGFP-tagged) - Mouse RIKEN cDNA 6720456B07 gene (6720456B07Rik), (10ug)
ORF Plasmid: DDK
"NM_133937" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 6720456B07Rik; AW011779 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG219863 representing NM_133937
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGACAAGAGGATCCGGTGCAGCGGGAGATTCACCAGGACTGGGCGAACCGGGAGTACATTGAGA TCATCACCAGCAGCATCAAGAAAATCTCAGACTTTCTCAACTCTTTCGATATGTCTTGTCGTTCAAGACT CGCAACACTAAATGAGAAATTGACAGCCCTTGAACGGAGAATAGAGTACATTGAAGCGAGGGTGACAAAG GGTGAGACTCTGACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219863 representing NM_133937
Red=Cloning site Green=Tags(s) MAGQEDPVQREIHQDWANREYIEIITSSIKKISDFLNSFDMSCRSRLATLNEKLTALERRIEYIEARVTK GETLT TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_133937 |
ORF Size | 225 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133937.1, NP_598698.1 |
RefSeq Size | 1110 bp |
RefSeq ORF | 228 bp |
Locus ID | 101314 |
UniProt ID | Q91VR8 |
Gene Summary | Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (PubMed:27605705).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212070 | Brk1 (untagged) - Mouse RIKEN cDNA 6720456B07 gene (6720456B07Rik), (10ug) |
CNY 3,990.00 |
|
MR219863 | Brk1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 6720456B07 gene (6720456B07Rik) |
CNY 1,200.00 |
|
MR219863L3 | Lenti ORF clone of Brk1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 6720456B07 gene (6720456B07Rik) |
CNY 4,750.00 |
|
MR219863L4 | Lenti ORF clone of Brk1 (mGFP-tagged) - Mouse RIKEN cDNA 6720456B07 gene (6720456B07Rik) |
CNY 4,750.00 |