Spg7 (NM_153176) Mouse Tagged ORF Clone
CAT#: MG219895
- TrueORF®
Spg7 (tGFP-tagged) - Mouse spastic paraplegia 7 homolog (human) (Spg7) nuclear gene encoding mitochondrial protein, (10ug)
ORF Plasmid: DDK
"NM_153176" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,370.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AI452278; AU015315; Cmar; PGN |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG219895 representing NM_153176
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGCCTTGCTGCTGCTTCGCGGGCTCCGCCCCGGCCCGGAGCCCCGGCCCCGACGGCTGTGGG GCCTGCTCTCAGGCAGGGGCCCAGGCCTCTCGTCCGGGGCTGGGGCCAGGCGGCCGTACGCGGCCCGCGG GACTCCGGTTGGCCCGGCTGCGGCTGGAGGCCACGCGCCTCAGAGTTTGCTATTGCGAATACTGACCCCT AGCTTTGAAGGTATTAGCGGATTGTTACTGAAACAGCATATAGTTCCAAATGCAGTCAGACTGTGGCCGC TTTCAGGTAGCACTTTGTATTTTAACACCTCAAGGATGAAGCAGAAGAATAAAGACAATGATAAACCCAA AGGCAAGACCCCTGAAGATGATGAAGAAGAGAAGAGACGAAAAGAGCGGGAAGACCAGATGTACCGAGAG AGGCTGCGCACCCTGTTCATCATTGCTCTTGTCATGAGCCTGCTGAACTCCCTCAGTACAAGTGGGGGCA GTATTTCCTGGTCTGACTTTGTCAACGAGATGCTGGCTAAAGGCGAGGTGCAGCGTGTGCAGGTGGTGCC CGAGAGTGATGTGGTGGAAGTCTATCTGCATCCTGGAGCTGTGGTGTTTGGGCGGCCTCGGCTGGCCCTG ATGTATCGGATGCAGGTTGCAAACATCGACAAATTTGAAGAGAAGCTTCGAGCAGCCGAAGATGAACTGA ACATTGAGAGCAAGGACAGGATTCCCGTGTCCTACAAGCGGACAGGATTCTTTGGGAATGCCCTCTACGC CCTGGGGATGACAGCCGTGGGCTTGGCCATCCTGTGGTATGTTTTCAGACTGGCGGGGATGACCGGAAGG GAAGGCGGATTCAGTGCTTTTAATCAGCTTAAGATGGCACGTTTCACCATTGTGGACGGGAAGACAGGGA AAGGAGTCAGCTTCCAAGATGTGGCAGGAATGCATGAAGCCAAGCTGGAAGTCCGAGAATTTGTGGATTA TCTGAAGAGCCCAGAGCGTTTCCTTCAGCTCGGTGCCAAGGTTCCAAAGGGTGCCCTGTTGCTGGGGCCC CCTGGCTGTGGGAAGACGCTGTTGGCCAAGGCAGTAGCCACGGAGGCTCAGGTGCCCTTTTTAGCAATGG CTGGCCCAGAGTTTGTGGAGGTGATTGGAGGCCTGGGAGCTGCCCGAGTGCGAAGCCTCTTCAAGGAGGC ACGAGCCAGGGCCCCTTGCATAGTGTACATTGATGAGATCGATGCTGTGGGAAAGAAGCGCTCCACCTCC ATGTCTGGGTTCTCCAACACGGAAGAGGAGCAGACCCTCAACCAGCTCCTGGTGGAGATGGACGGAATGG GCACCACAGACCATGTCATCGTCTTGGCATCCACCAATCGAGCTGATGTTCTGGACAATGCTCTGATGAG GCCTGGGCGGCTCGACAGGCATGTCTTCATTGATCTTCCCACGCTCCAGGAGAGGCGGGAGATTTTCGAG CAGCACCTGAAAGGCCTGAAGTTGACCCAGCCCAGCAGTTTTTACTCCCAGCGGCTGGCAGAGCTGACAC CTGGATTCAGTGGCGCCGACATCGCCAACATCTGCAACGAGGCCGCTCTGCACGCTGCACGCGAGGGGCA CACGTCCGTGCACACGTTCAACTTTGAGTATGCCGTGGAGCGCGTCATTGCTGGTACTGCTAAAAAGAGT AAGATCCTCTCCAAGGAGGAGCAGAGAGTGGTCGCCTTCCATGAGTCTGGCCATGCCTTGGTTGGTTGGC TGCTGGAGCACACAGAGGCTGTGATGAAGGTCTCCATCGCACCTCGGACAAACGCTGCTTTGGGCTTCTC TCAGATGCTCCCTCGGGACCAGTACCTCTTCACCAAGGAGCAGCTGTTTGAGCGCATGTGCATGGCCCTA GGTGGCCGTGCAGCTGAAGCCATCTCATTCAGCAGGGTCACCTCTGGGGCCCAGGATGATTTGAGGAAGG TTACCCGCATTGCTTACTCCATGGTGAAACAGTTCGGGATGGCTCCCAGCATTGGGCCTGTATCCTTCCC CGAGGCACAAGAGGGCCTCATGGGCATTGGACGTCGTCCCTTCAGCCAGGGCCTCCAGCAGATGATGGAC CATGAAGCAAAGCTGCTGGTGGCCAAAGCCTACAGACACACCGAGAAGGTGCTGCTAGACAACCTGGACA AGCTGCAGGCGTTGGCGAATGCCCTTCTGGAAAAGGAAGTGATAAACTATGAGGACATTGAGGCGCTCAT TGGCCCGCCGCCCCATGGGCCAAAGAAAATGATTGCACCACAGAAATGGATTGATGCTGAAAAGGAGAGA CAGGCCTCAGGGGAGGAGGAGGCTCCGGCTCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219895 representing NM_153176
Red=Cloning site Green=Tags(s) MAAALLLLRGLRPGPEPRPRRLWGLLSGRGPGLSSGAGARRPYAARGTPVGPAAAGGHAPQSLLLRILTP SFEGISGLLLKQHIVPNAVRLWPLSGSTLYFNTSRMKQKNKDNDKPKGKTPEDDEEEKRRKEREDQMYRE RLRTLFIIALVMSLLNSLSTSGGSISWSDFVNEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLAL MYRMQVANIDKFEEKLRAAEDELNIESKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGR EGGFSAFNQLKMARFTIVDGKTGKGVSFQDVAGMHEAKLEVREFVDYLKSPERFLQLGAKVPKGALLLGP PGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTS MSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLASTNRADVLDNALMRPGRLDRHVFIDLPTLQERREIFE QHLKGLKLTQPSSFYSQRLAELTPGFSGADIANICNEAALHAAREGHTSVHTFNFEYAVERVIAGTAKKS KILSKEEQRVVAFHESGHALVGWLLEHTEAVMKVSIAPRTNAALGFSQMLPRDQYLFTKEQLFERMCMAL GGRAAEAISFSRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEGLMGIGRRPFSQGLQQMMD HEAKLLVAKAYRHTEKVLLDNLDKLQALANALLEKEVINYEDIEALIGPPPHGPKKMIAPQKWIDAEKER QASGEEEAPAP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_153176 |
ORF Size | 2343 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153176.4, NP_694816.3 |
RefSeq Size | 2558 bp |
RefSeq ORF | 2346 bp |
Locus ID | 234847 |
UniProt ID | Q3ULF4 |
Gene Summary | ATP-dependent zinc metalloprotease. Plays a role in the formation and regulation of the mitochondrial permeability transition pore (mPTP) and its proteolytic activity is dispensable for this function (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221597 | Spg7 (untagged) - Mouse spastic paraplegia 7 homolog (human) (Spg7), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 10,070.00 |
|
MR219895 | Spg7 (Myc-DDK-tagged) - Mouse spastic paraplegia 7 homolog (human) (Spg7), nuclear gene encoding mitochondrial protein |
CNY 5,728.00 |
|
MR219895L3 | Lenti ORF clone of Spg7 (Myc-DDK-tagged) - Mouse spastic paraplegia 7 homolog (human) (Spg7), nuclear gene encoding mitochondrial protein |
CNY 7,700.00 |
|
MR219895L4 | Lenti ORF clone of Spg7 (mGFP-tagged) - Mouse spastic paraplegia 7 homolog (human) (Spg7), nuclear gene encoding mitochondrial protein |
CNY 7,700.00 |