Gpr68 (NM_001177673) Mouse Tagged ORF Clone
CAT#: MG220141
- TrueORF®
Gpr68 (tGFP-tagged) - Mouse G protein-coupled receptor 68 (Gpr68) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001177673" in other vectors (4)
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CNY 3,140.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BB131428; Ogr1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG220141 representing NM_001177673
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAACATCACTACAGAAAACTCCTCACTATCTTGCCCCATCGACCACACCATCCACCAGACACTAG CCCCAGTGGTCTATGTGACCGTGCTGGTGGTGGGCTTCCCAGCCAACTGCCTGTCCCTCTACTTCGGGTA CTTGCAGATCAAGGCCCGGAATGAGCTGGGAGTGTACCTGTGTAACCTGACCATTGCAGACCTGTTCTAT ATCTGTTCACTTCCCTTCTGGCTGCAGTACGTGCTTCAGCACGACGACTGGTCCCATGGTGACCTATCCT GCCAGGTGTGTGGCATCCTCCTCTATGAGAACATTTACATCAGCGTGGGCTTCCTCTGCTGCATCTCCAT CGACCGCTACCTGGCTGTGGCCCACCCCTTCCGCTTCCACCAGTTCCGCACCCTGAAGGCAGCCGTGGGT GTCAGTGTGCTCATCTGGGCCAAGGAGCTGCTGACCAGCATCTACTTCCTCAATCACAAGGAGGTCATTG AGGACGAGGACCAGCACCGAGTCTGCTTTGAGCATTACCCTATCCAGGCCTGGCAGCGTAGCATCAACTA CTACCGCTTCCTGGTGGGCTTTCTCTTCCCCATCTGCCTGCTGCTGGCCTCCTACCAGGGCATCCTGCGG GCTGTGCGCCGCAGCCACGGCACACAGAAGAGCCGCAAGGACCAGATTCAGCGGCTGGTGCTCAGCACCG TGGTCATCTTCCTGGCTTGCTTTCTACCCTACCACGTGCTGCTGCTGGTACGCAGCCTCTGGGAGAGAAA CTGTGAGTTTGCCAAGAGCATCTTCAACGTCTATCACTTCTCCCTCCTCCTCACCAGCTTCAACTGTGTA GCTGACCCGGTGCTGTACTGCTTTGTCAGTGAGACCACTCACAGGGACCTAGCCCGCCTCCGAGGAGCCT GCCTAGCTGTCCTTACCTGCTCTAGGACAAGCAGGGCCAGGGAGGCCTACCCTCTGGGTGCCCCTGAGGC CTCTGGGAAAAGTGGGGCCCAGGGCGAGGAACCTGAATTGTTAACCAAGCTCCACTCAGCCTTCCAGACC CCTAGCTCACTGGGAGTGGGAGGGCCCTCCACAGTGGGGTTGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220141 representing NM_001177673
Red=Cloning site Green=Tags(s) MGNITTENSSLSCPIDHTIHQTLAPVVYVTVLVVGFPANCLSLYFGYLQIKARNELGVYLCNLTIADLFY ICSLPFWLQYVLQHDDWSHGDLSCQVCGILLYENIYISVGFLCCISIDRYLAVAHPFRFHQFRTLKAAVG VSVLIWAKELLTSIYFLNHKEVIEDEDQHRVCFEHYPIQAWQRSINYYRFLVGFLFPICLLLASYQGILR AVRRSHGTQKSRKDQIQRLVLSTVVIFLACFLPYHVLLLVRSLWERNCEFAKSIFNVYHFSLLLTSFNCV ADPVLYCFVSETTHRDLARLRGACLAVLTCSRTSRAREAYPLGAPEASGKSGAQGEEPELLTKLHSAFQT PSSLGVGGPSTVGLA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001177673 |
ORF Size | 1095 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001177673.1, NP_001171144.1 |
RefSeq Size | 2938 bp |
RefSeq ORF | 1098 bp |
Locus ID | 238377 |
UniProt ID | Q8BFQ3 |
Gene Summary | Proton-sensing receptor involved in pH homeostasis. May represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone. Mediates its action by association with G proteins that stimulates inositol phosphate (IP) production or Ca(2+) mobilization. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Function also as a metastasis suppressor gene in prostate cancer.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC213053 | Gpr68 (untagged) - Mouse G protein-coupled receptor 68 (Gpr68), transcript variant 2, (10ug) |
CNY 3,656.00 |
|
MR220141 | Gpr68 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 68 (Gpr68), transcript variant 2 |
CNY 3,656.00 |
|
MR220141L3 | Lenti ORF clone of Gpr68 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 68 (Gpr68), transcript variant 2 |
CNY 4,750.00 |
|
MR220141L4 | Lenti ORF clone of Gpr68 (mGFP-tagged) - Mouse G protein-coupled receptor 68 (Gpr68), transcript variant 2 |
CNY 4,750.00 |