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Sars (NM_011319) Mouse Tagged ORF Clone
CAT＃: MG220183
- TrueORF®
Sars (tGFP-tagged) - Mouse seryl-aminoacyl-tRNA synthetase (Sars), (10ug)
ORF Plasmid: DDK
"NM_011319" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Sars1; serRS; Strs |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220183 representing NM_011319
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGGACCTGGATTTGTTTCGGGTGGATAAAGGAGGGGACCCAGCCCTCATTCGAGAGACGCAGG AGAAGCGCTTCAAGGACCCGGGGCTGGTGGACCAGCTGGTGAAAGCAGACAGTGAGTGGCGACGATGCAG ATTTCGGGCAGACAACTTGAACAAGCTGAAGAATTTATGCAGCAAAACTATTGGGGAGAAAATGAAGAAA AAGGAAGCAGTGGGAGACGACGAGTCCGTCCCAGAGAACGTGCTGAATTTCGATGACCTCACTGCAGACG CGCTAGCTGCCCTGAAAGTCTCACAGATTAAAAAAGTCCGACTCCTCATTGATGAAGCCATCCAGAAGTG TGATGGGGAGCGGGTAAAGCTGGAAGCAGAGCGATTTGAGAACCTCCGCGAGATTGGGAACCTTCTGCAC CCCTCTGTGCCCATTAGTAATGATGAGGACGCAGACAACAAAGTAGAACGTATTTGGGGAGATTGTACAG TCAGGAAGAAGTATTCCCATGTGGACCTGGTGGTGATGGTAGATGGCTTTGAAGGCGAAAAGGGAGCCGT GGTGGCTGGTAGTCGGGGGTACTTCCTGAAGGGGCCCCTGGTGTTCCTGGAGCAGGCGCTTATCCAATAT GCACTGCGTACCTTGGGGAGTCGGGGCTACACTCCAATCTACACCCCCTTCTTCATGAGGAAAGAGGTCA TGCAGGAAGTGGCCCAGCTCAGCCAGTTTGATGAAGAACTTTATAAGGTGATTGGCAAAGGCAGCGAAAA GTCAGATGACAACTCCTATGACGAGAAATACTTGATTGCCACCTCAGAGCAGCCCATCGCGGCTCTGCAC CGGGACGAGTGGCTGCGGCCAGAGGATCTGCCCATCAAGTACGCTGGCCTCTCCACCTGCTTTCGTCAGG AAGTGGGCTCGCATGGCCGTGACACCCGTGGTATCTTCCGAGTCCATCAGTTTGAGAAGATTGAGCAGTT TGTGTACTCATCGCCCCATGACAATAAGTCGTGGGAGATGTTTGATGAGATGATCGCCACCGCAGAAGAA TTCTACCAGTCTTTGGGGATCCCTTACCACATTGTGAATATTGTCTCAGGCTCCTTGAATCACGCTGCCA GTAAGAAGCTCGACCTGGAGGCCTGGTTCCCAGGCTCGGGTGCCTTCCGTGAGTTGGTGTCCTGTTCTAA TTGCACGGATTACCAAGCTCGCCGCCTGAGAATCCGATATGGGCAGACCAAGAAGATGATGGACAAGAAA AAGAATAATCTCCGTTTCTCTACACAGAACAAGTTGAAGGCAATGTCTTCCCTCTTTTCGCCCAAGAAGG TGGAGTTTGTCCATATGCTTAATGCTACAATGTGTGCTACCACCCGGACCATCTGCGCCATCCTGGAGAA CTACCAGGCAGAGAAGGGCATCGCTGTGCCAGAGAAGTTGAGGGAGTTCATGCCGCCAGGGCTCCAAGAG CTGATCCCGTTTGTGAAGCCTGCACCCATTGACCAGGAGCCATCTAAGAAGCAGAAGAAGCAACATGAAG GCAGCAAAAAGAAAGCGAAAGAGGTCCCCCTGGAGAACCAGCTGCAGAGCATGGAGGTCACTGAGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220183 representing NM_011319
Red=Cloning site Green=Tags(s) MVLDLDLFRVDKGGDPALIRETQEKRFKDPGLVDQLVKADSEWRRCRFRADNLNKLKNLCSKTIGEKMKK KEAVGDDESVPENVLNFDDLTADALAALKVSQIKKVRLLIDEAIQKCDGERVKLEAERFENLREIGNLLH PSVPISNDEDADNKVERIWGDCTVRKKYSHVDLVVMVDGFEGEKGAVVAGSRGYFLKGPLVFLEQALIQY ALRTLGSRGYTPIYTPFFMRKEVMQEVAQLSQFDEELYKVIGKGSEKSDDNSYDEKYLIATSEQPIAALH RDEWLRPEDLPIKYAGLSTCFRQEVGSHGRDTRGIFRVHQFEKIEQFVYSSPHDNKSWEMFDEMIATAEE FYQSLGIPYHIVNIVSGSLNHAASKKLDLEAWFPGSGAFRELVSCSNCTDYQARRLRIRYGQTKKMMDKK KNNLRFSTQNKLKAMSSLFSPKKVEFVHMLNATMCATTRTICAILENYQAEKGIAVPEKLREFMPPGLQE LIPFVKPAPIDQEPSKKQKKQHEGSKKKAKEVPLENQLQSMEVTEA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011319 |
| ORF Size | 1608 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011319.3, NP_035449.1 |
| RefSeq Size | 1866 bp |
| RefSeq ORF | 1611 bp |
| Locus ID | 20226 |
| Gene Summary | Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC. Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217577 | Sars (untagged) - Mouse seryl-aminoacyl-tRNA synthetase (Sars), transcript variant 1, (10ug) |
CNY 6,840.00 |
|
| MR220183 | Sars (Myc-DDK-tagged) - Mouse seryl-aminoacyl-tRNA synthetase (Sars), transcript variant 1 |
CNY 3,992.00 |
|
| MR220183L3 | Lenti ORF clone of Sars (Myc-DDK-tagged) - Mouse seryl-aminoacyl-tRNA synthetase (Sars), transcript variant 1 |
CNY 6,460.00 |
|
| MR220183L4 | Lenti ORF clone of Sars (mGFP-tagged) - Mouse seryl-aminoacyl-tRNA synthetase (Sars), transcript variant 1 |
CNY 6,460.00 |
