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Thada (NM_183021) Mouse Tagged ORF Clone
CAT＃: MG220303
- TrueORF®
Thada (tGFP-tagged) - Mouse thyroid adenoma associated (Thada), (10ug)
ORF Plasmid: DDK
"NM_183021" in other vectors (2)
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CNY 15,580.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6530405K19; A930021O22; AU019899; BC052885; GITA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220303 representing NM_183021
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGTGAAGAAGAAAAGAGAAATGCAGGTTGCTGCGCTGACTGTCTGTCATCAGGACATGGAAACTT TGAGATCTTTTGCTGATATGGAAGGGAAAAATCTAGCCTCTTTGCTGTTACATTGTGTACAGCTCACAGA TGGAGTGTCACAAATCCACTCTATTAAACAGATTGTGCCTCTCTTGGAGAAGGTCGATAAAAACGGTGTG TGCGATCCTGCTATTCAGAGTTGTTTGGATATTCTAGCAGGCATTTATTTTTCCTTGACTCTGAAGAACC CCTTGAAGAAAGTTTTGGCCAGCTCACTCAATGGCCTACCTGAGGTTTTTCTAACTCAGGCAACACACAG TTTTACTTTTCATCTTCAAGAAGAATTAGATACTGCTGACTTATATTCTTATAGAAAAGTAATGGATAAC ATATCTTCCTGCATGGAGAACTTTAACCTGGGTAGAGCAAGTGTGGTTAATCTGCTTAAAGATGTGCTTC ATTTTCTGCAGAAGAGTTTAATTGAAATCCTGGAAGAAAACAGGAAGTTTGCTGGAAATCGTATTGTTCA AACACAGTTAATGAGTGACTTGCTGGTAGGTGTTAGAGTTGCCATGACATTGGTACAGAAAGTACAGGGG CCCCAGGGCAGCCTCTGGAATGACTCCAGCTCACCCATCTGGCAGAGTATGTGTGGCTTGCTGAGCATCT TCACCAAGTTCTTAAATGACGATGACCTACTGCAGACTGTTGAGAGTACATCTGGATTAGCTGTTATTCT TTTCATTAAGACCATGTTCCGGCCATCTGAAAAGCTTCCTGGATTGATCAGCAGCTTGCTTCTCCGGTCT GCTGAGTGCACTAGCATCCCTGAGTGGCTCATGAATAGCTGTAGGAGCCTTTGCTGTACTGATGTGCCAG CCTCAACTCTCTTATTCCTGTGTCAGGGAACACTTGCTATGCTGGACTGGCAGGATGGGAGCATGGGCCC CAGCGGGGAGGCTTTGCTCTTGGATATAGTGCATGTTTTGTTCACCTTGAGTTCACAGATTAAGGAGTCC ACATTAGATATGTTCCTGTCTAGAATCCTGGCATCTTGGACCAGTTCAGCCATACAGATCCTTGAATCAG GCTCCTCAGGCTTAAAGGGTCATCTGAACGGGGACTCATGCGTACCTAGGAGACTTCTGGAGTATGTCTA CACACACTGGGAGCACCCGTTAGACGCTCTGAGACATCAAACCAAAGTCATGTTCAGGAATCTCTTGCAA ATGCACCGGCTCACTATGGAAGGGGCAGATTTGGCCACTGACCCCTTCTGTCTGGAGTTGACTAAGAGTC TTCTGCAGCTGGAATGGCACATTAAAGGGAAGTACGCTTGCCTTGGCTGTTTGGTGGAGACTCTTGGAAT TGAACATATTTTGGCAATAGATAAAACCATTCCATCTCAAATTCTAGAGGTGATGGGAGACCAGTCATTG GTACCTTATGCAAGTGACCTCTTGGAAACCATGTTTAAAAATCATAAGAGCCATTTGAAGTCCCAAACTG TCACCAATACCTGGATGGACAAGTGGCATGAGACTTGGGTTTTCCCTGTCCTTTCTGTACTGTGTGGAGG AAACTTGGATCAGAGATCTTATGTGATTGATTATTACCTGCCACGGATACTGAATTACAGCCCCGAGAGC CTGCACTACATGGTGCACATTCTTCAGGCATCTACTGATACTGGAACTGGGTCTTGTAATCACAGAGGAG CTCTGGGAGCTTTGATGGCGTGTCTCCGAACAGCTAGAGCTCATGGACATCTTCAGTCTGCAACTCAGGC CTGGGAGAACCTTGTGTGCAGTGCAAGGGTAAAGCAAGGCTTAATTCACCAACACTGCCAAGTGCGGATA GATACACTAGGCTTGCTTTGTGAAAGTAATCGGAGTACAGAAGTGGTCTCTACAGAAGAAATGCAGTGGG TTCAGTTTTTTATCACTTACAACCTCAACAGCCAGTCTCCAGGGGTGCGCCAGCAGATTTGCTCACTCCT TAAAAAGTTGTTTTGCAGGATACAAGAAAGTTCACAGGTACTTTATAAATTGGAACAGAGAAAATCTACA CCTGACTCAGAGAACGGGTCCATCAGAGAGCAGCCTTCTGTCACTTTACAGCAGTATAAGAATTTCATGT CGTCTGTTTGCAACATTCTCTTTGAAGCATTGTTTCCTGGGTCTTCCTACTCGACTAGATTTTCAGCTTT AACTATCTTAGGATCAGTAGCTGAAGTGTTTCCTGATCCAGAAGGTAACATCCAAACAGTGTATCAGCTG AGTCATGATATTGATGCTGGCCGTTACCAAATACTTATGGAGTGTTTCACTAGTACTTTTGAAGAAGTGA AAACCTTAGCATTTGATCTTCTGATGAAGCTGTCGTCAGTAACAGCTGGACAGTTCCAGGATTCTGAGAA GCTGCAAGACCTGTTCCAGGCAGCGCTGGAACTCAGCACTAGCACCAAGCCTTATGATTGTGTGACAGCC TCCTACCTGCTGAACTTGCTGATACGGCAGGATGCTCTGCCAGCCGTCCTGTCTGCCTCTTCCCCACAGC AGCTCACACGTGGAGCAGGAGAGACGTCTGCTGTGCTGGAAAGGAACACATTAGTGGTTATTAAATGCTT GATGGAAAACCTTGAGGATGAAATATCTCAAGCTGAGAACTCTCTGCTTCAGGCTGCGTCTTCATTTCCA ATGTATGGGAGAGTCCACTGTATAACAAGAGCTTTTCAGAGGTTGCCTCTAAATGACCTGCGGCTGGCGA GCGAGTGGAGGCCGCTGCTGGGGAGGCTTCTGCTGCTGTCCTACAGGCTTTCCACTGTGGTGGCCCCTGT CATCCAGAGCTCCTCTCCTGAAGGCCTCATCCCTGTGGACACTGACTCAGCGTCAGCAAGCCGCTTGCAG CTCATTCTCAATGAGATCCAGCCACGGGATACTAACGATTACTTCAACCACACCAAAATACTGAAAGAAT GTGATAGCTTTGATTTGGAGGACTTGAGTACCAGTGTCTCAAATATTGATAGTTCTGCAGAAGTTAAAGG TAAAGAAGAAAAAGCGTGTGACGTAACTGCTCAGATGGTCCTCGCGTGCTGCTGGAGGAGCATGAAGGAA GTGGCTTTGCTTCTCGGCACGCTCTGCCAGCTTCTCCCTGTGCAGCCTGGGCCAGAATCTTCTAATGTGT TCCTAACAGTGCAGCAGGTTAAAGAAATAGGAGATTACTTTAAACAACACCTTTTGCAGTCCAGACACAG AGGAGCATTTGAACTGGCTTATACTGGTTTTGTGAAACTCACTGAAATATTGAACAGGTGCTCTAATGTG AGTCTGCAAAAGCTGCCAGAGCAGTGGCTGCGGAGCGTTCTGGAGGAAATTAAAGGCAGTGATCCCTCAT CCAAACTTTGTGCTACAAGGCGCAGTGCTGGGATTCCCTTCTACATACAGGCATTGTTGGCATCTGAACC AAAGAAGAGTAGAATGGACTTGCTGAAAATAACAATGAGGGAGCTGATCTCCTTGGCTTTGTCTGCAGAT GACTCAAAGGGCAGAGTCCCTCAGGTTCATGCTTTAAACATTCTCCGAGCCTTGTTCAGAGACACCCGTC TGGGAGAAAACATTATCCCCTATGTTGCTGGTGGAGCTAAGGCTGCAATTCTGGGCTTTACATCCCCTGT CTGGGCAGTGAGAAATTCATCCACACTTCTCTTTAGTTCCTTGATCACAAGAGTTTTTGGCGTTAAGAGA GGAAAGGATGAAGTTTCCAAAACAAATAGAATGACAGGAAGAGAGTTTTTCTCTCGTTTCCCAGAACTGT ACCCATTTCTTCTCAAACAGTTGGAAACTGTGGCCAGTACAGTAGACAGTGAACTGGGAGAGCCTGATCG CCACCCTGGCATGTTCCTCTTACTCCTGGTGTTGGAGAGACTGTACCCATCGCCGATGGACGGCACATCA TCTGCGCTCAGCTTGGCCCCTTTTGTTCCTTTCATTATCAGGTGTGGTCGCTCACCTATTTACCGCTCCC GGGAAATGGCAGCTCGTGCGTTGGTCCCCTTCATTATGATAGACCAAATACCGAGTACCCTCTGTGCTCT ACTGAACTCACTCCCCAACAGCACTGACCAGTGTTTCCGACAAAACCACATTCATGGGACACTTCTGCAA GTTTTTCATCTGTTACAAGCCTACATTACAGACTGCAGACACAGAACAAATGCAGATTTCCTGCAAGAGC TGAGTGATGTCACTGCTTGTACCAAAGCCAAACTCTGGCTGGCCATGAGGCAAAATCCATGCCTGGTAAC CAGAGCTGTGTATATTGATATTCTCTTCCTGCTGACTAACTGCCTGGACAGACCTGAGGAAGGCAAGCAG ACAGCTCTGGAGAGTCTTGGCTTCTGGGAAGATGTGAGAAGAATTATCCTGGAATCAGAGCTGATTAAGG GGTTCCCCTGGACCTTCAAGGTGCCAGGCTTGCCCCAGTATCTGCAGAGCCTTACCAAACTAGCCATCCC TGAAGTGTGGGCGTCGCTTGCTGAAGCTAAAGGGCAGGCGACTGCTGTTCCTCTCTCCTTCTCTCGGCTT CTGAAGTCTTCCTTTCCTGAAGTGCGCTTGCTAGCCCTAGACACCTTGCTGGAAAGGGCAAGGTCCTCTG AACAGGAGCAGAAGGAGCCACTTCCTCTGCTCTGCAGTATGGGAGAGGAGCTTCTACTGCTGGCGATGAA GGAAGACCACCCCGGATGTTTCTGCAGGGTCCTAAAGATTCTCTATCACTTGAACCCCAGCGAATGGCTT CCCCAGACAGAGTGCTATGCTCACCTCTCCCCAAAGGAGTTCCTCATGTGGAGTATGGATATTGCTTCCA ATGACAGATCTGAAATCCAGGGAGTAGCTCTGAAGCTTGCCTCTAAAATAATTGCCTACCGTGTACAGAG TTGCGAGAAGAACAAAGATTCACTCGCCCCTGAGCTGAGGCAGTGGGTCCAGCTGGTTGTCTGGTCCTGT GGAGACCATCTTCCTACTGCATCTAGACTGGCTGTTGCTGAAGTCCTCACAAGCACCGCACCCCTGTTCC TCACCAGTCCCCAGCCCATTCTCGAACTGCAGGGCACACTTTCTCTCTGGAGGTGTGTTCTTACCCTTCT GCAGAGTGAGGAGCAAACCGTCAGAGAGGCAGCCACAGAAATCGTGACAACAGCCATGTCACAAGGAAAC ACTTGCCAGTCAACAGAATTTGCCTTCTGCCAGGTGGATGCCTCCATTGCTCTGACCCTGGCCTTAGCTG TTCTGTGTGACTTGCTCCAGCAGTGGGACCAGCTGGAGCCAGGACTGCCTATTCTACTGGGATGGCTACT GGAAGAGGGTGATGACCTCGAGGGCCACGTGCAGAGCCCACATCAGGGGGAAGAAGAGCATATCTTTGAA AAATCAGAAGTCAACTTCTGGGCTGAGACTCTGACCTTCGTGAAATCCCTGTGTAGGCAACTCTTCCATC TCCTCTGTCAATCTGGCTGGCAGTCCCCTCACTCCCAGAAGCTCTGTCACTTGCAAAGGATAGCATCAGA GCAGAGTCACCTCATCTCTCAGCTCTTCAGAGAACTTCCACTCTCAGCTGAGTTTTTGAAGACAGTGGAA TACACGAGGCTGCGCATTCAGGAGGAAAGGACTTTGGCTGTCCTGAGGCTGTTGGCCTGCCTGGAAGGAA AGGAGGGTCTCAGAGCCGAAGACTGCCCTAGGGAATGGAGACAAGTAATGGCCCCAAGAACAGAAGCAGC ATGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220303 representing NM_183021
Red=Cloning site Green=Tags(s) MGVKKKREMQVAALTVCHQDMETLRSFADMEGKNLASLLLHCVQLTDGVSQIHSIKQIVPLLEKVDKNGV CDPAIQSCLDILAGIYFSLTLKNPLKKVLASSLNGLPEVFLTQATHSFTFHLQEELDTADLYSYRKVMDN ISSCMENFNLGRASVVNLLKDVLHFLQKSLIEILEENRKFAGNRIVQTQLMSDLLVGVRVAMTLVQKVQG PQGSLWNDSSSPIWQSMCGLLSIFTKFLNDDDLLQTVESTSGLAVILFIKTMFRPSEKLPGLISSLLLRS AECTSIPEWLMNSCRSLCCTDVPASTLLFLCQGTLAMLDWQDGSMGPSGEALLLDIVHVLFTLSSQIKES TLDMFLSRILASWTSSAIQILESGSSGLKGHLNGDSCVPRRLLEYVYTHWEHPLDALRHQTKVMFRNLLQ MHRLTMEGADLATDPFCLELTKSLLQLEWHIKGKYACLGCLVETLGIEHILAIDKTIPSQILEVMGDQSL VPYASDLLETMFKNHKSHLKSQTVTNTWMDKWHETWVFPVLSVLCGGNLDQRSYVIDYYLPRILNYSPES LHYMVHILQASTDTGTGSCNHRGALGALMACLRTARAHGHLQSATQAWENLVCSARVKQGLIHQHCQVRI DTLGLLCESNRSTEVVSTEEMQWVQFFITYNLNSQSPGVRQQICSLLKKLFCRIQESSQVLYKLEQRKST PDSENGSIREQPSVTLQQYKNFMSSVCNILFEALFPGSSYSTRFSALTILGSVAEVFPDPEGNIQTVYQL SHDIDAGRYQILMECFTSTFEEVKTLAFDLLMKLSSVTAGQFQDSEKLQDLFQAALELSTSTKPYDCVTA SYLLNLLIRQDALPAVLSASSPQQLTRGAGETSAVLERNTLVVIKCLMENLEDEISQAENSLLQAASSFP MYGRVHCITRAFQRLPLNDLRLASEWRPLLGRLLLLSYRLSTVVAPVIQSSSPEGLIPVDTDSASASRLQ LILNEIQPRDTNDYFNHTKILKECDSFDLEDLSTSVSNIDSSAEVKGKEEKACDVTAQMVLACCWRSMKE VALLLGTLCQLLPVQPGPESSNVFLTVQQVKEIGDYFKQHLLQSRHRGAFELAYTGFVKLTEILNRCSNV SLQKLPEQWLRSVLEEIKGSDPSSKLCATRRSAGIPFYIQALLASEPKKSRMDLLKITMRELISLALSAD DSKGRVPQVHALNILRALFRDTRLGENIIPYVAGGAKAAILGFTSPVWAVRNSSTLLFSSLITRVFGVKR GKDEVSKTNRMTGREFFSRFPELYPFLLKQLETVASTVDSELGEPDRHPGMFLLLLVLERLYPSPMDGTS SALSLAPFVPFIIRCGRSPIYRSREMAARALVPFIMIDQIPSTLCALLNSLPNSTDQCFRQNHIHGTLLQ VFHLLQAYITDCRHRTNADFLQELSDVTACTKAKLWLAMRQNPCLVTRAVYIDILFLLTNCLDRPEEGKQ TALESLGFWEDVRRIILESELIKGFPWTFKVPGLPQYLQSLTKLAIPEVWASLAEAKGQATAVPLSFSRL LKSSFPEVRLLALDTLLERARSSEQEQKEPLPLLCSMGEELLLLAMKEDHPGCFCRVLKILYHLNPSEWL PQTECYAHLSPKEFLMWSMDIASNDRSEIQGVALKLASKIIAYRVQSCEKNKDSLAPELRQWVQLVVWSC GDHLPTASRLAVAEVLTSTAPLFLTSPQPILELQGTLSLWRCVLTLLQSEEQTVREAATEIVTTAMSQGN TCQSTEFAFCQVDASIALTLALAVLCDLLQQWDQLEPGLPILLGWLLEEGDDLEGHVQSPHQGEEEHIFE KSEVNFWAETLTFVKSLCRQLFHLLCQSGWQSPHSQKLCHLQRIASEQSHLISQLFRELPLSAEFLKTVE YTRLRIQEERTLAVLRLLACLEGKEGLRAEDCPREWRQVMAPRTEAAC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_183021 |
| ORF Size | 5814 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_183021.3, NP_898842.2 |
| RefSeq Size | 7896 bp |
| RefSeq ORF | 5817 bp |
| Locus ID | 240174 |
| UniProt ID | A8C756 |
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