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Pitrm1 (NM_145131) Mouse Tagged ORF Clone
CAT＃: MG220510
- TrueORF®
Pitrm1 (tGFP-tagged) - Mouse pitrilysin metallepetidase 1 (Pitrm1) nuclear gene encoding mitochondrial protein, (10ug)
ORF Plasmid: DDK
"NM_145131" in other vectors (4)
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CNY 10,070.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310012C15Rik; AA410010; mKIAA1104; MP-1; MP1; Ntup1; PreP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220510 representing NM_145131
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCGCTTCAGCGGTCGGCGGGGACTCTGCGCTGTACAGCGGCTGAGCTGCGGCAGGGTACACCACA GAGTATGGAGGGAGAAGAGTGACCAAGCCTGTGAACGAGCTCTACAGTATAAAGTGGGAGAGAAAATCCA CGGGTTCACTGTAAACCAGGTCACTCCTGTCCCCGAGCTGTTCCTGACAGCCGTGAAGCTCAGCCATGAC AACACGGGAGCCAGATACCTGCACCTGGCAAGGGAAGACAAGAACAACTTATTCAGTGTGCAGTTCCGCA CAACCCCAATGGATAGCACTGGGGTCCCACATGTTCTCGAGCATACGGTCCTGTGCGGCTCTCAGAAGTA CCCGTGCAGAGATCCTTTCTTCAAAATGCTCAACAGGTCACTGTCCACATTTATGAATGCCATGACAGCC AGCGATTACACGATATATCCGTTTTCCACTCAAAATCCCAAAGATTTTCAGAACCTCCTCTCCGTGTATT TGGATGCAACTTTCTTCCCCTGCTTGAGGGAACTGGACTTCTGGCAGGAAGGATGGCGTCTGGAGCATGA GAATCCCCGAGACCCTCAGACGCCCTTGATCTTTAAGGGGGTCGTCTTCAACGAGATGAAAGGGGCATTT ACAGACAATGAGAGGATATTCTCCCAGCACCTGCAGAACAAGCTGCTTCCTGACCACACCTACTCCGTGG TTTCTGGAGGGGACCCACTGTGCATCCCGGAGCTCACGTGGGAACAGCTGAAACAGTTCCACGCTACTCA TTATCACCCAAGCAATGCCAGGTTCTTCACTTATGGCAATTTTCAGCTGGAAGGACACCTGAAACAAATT CACGAAGAAGCCCTGAGTAAATTCCAGAGATTGGAGCAGAGTACAGCAGTGCCTGCCCAGCCGCACTGGG ATAAGCCTAGGGAATTCCATATAACATGTGGCCCAGATTCACTAGCTACGGAGACTGCCAAGCAGACAAC TGTCAGCGTTAGCTTCCTCTTACCGGATATCACTGACACATTTGAAGCCTTCACCTTGAGCCTTCTGTCC TCCCTCCTGATTGCTGGACCCAACTCCCCCTTCTACAAAGCTTTGATCGAGTCTGGACTCGGCACAGACT TTTCTCCTGATGTTGGATATAATGGCTATACACGGGAGGCTTACTTCAGTGTCGGGCTCCAAGGGATCGC AGAGAAAGATGTCAAGACGGTCAGAGAGCTCGTAGACAGGACAATCGAAGAAGTTATAGAGAAAGGATTT GAAGATGATCGGATTGAAGCTCTGCTTCATAAAATCGAAATTCAAACGAAGCATCAGTCAGCCAGCTTTG GCCTGACCCTGACGTCATATATAGCTTCTTGCTGGAACCATGATGGGGACCCTGTGGAGCTCCTGCAGAT TGGAAGTCAGCTGACTAGATTTAGGAAGTGCCTTAAGGAAAATCCAAAATTTTTACAAGAAAAAGTAGAA CAATATTTTAAGAACAATCAGCACAAGCTGACTTTATCCATGAAGCCAGACGACAAGTATTATGAAAAGC AAACTCAGATGGAGACAGAAAAGCTGGAGCAAAAGGTGAATTCTCTCTCCCCGGCGGACAAGCAGCAGAT CTACGAGAAAGGTTTAGAACTACAGACGCAGCAAAGTAAACATCAAGACGCCTCCTGCCTCCCAGCATTG AAAGTCTCGGACATTGAGCCCTCCATGCCTTTCACCAAGCTTGACATCGGCCTTGCAGCTGGAGACATCC CTGTGCAGTACTGCCCACAGCCCACCAACGGCATGGTGTATTTCCGAGCCTTTTCCAGTTTAAACACGCT GCCGGAGGACCTGAGGCCCATTGTGCCTCTCTTTTGCAGCGTGCTGACCAAGCTGGGTTGTGGCATCCTT AACTACAGAGAGCAAGCCCAACAGATTGAGCTCAAGACAGGAGGCATGAGTGTCACGCCCCATGTGCTCC CTGACGACTCACAGCTGGATACCTACGAGCAGGGTGTGTTATTTTCATCTCTCTGCCTGGAGCGGAACCT GCCAGACATGATGCATCTGTGGAGCGAAATATTTAACAATCCATGCTTTGAAGAAGAAGAACACTTCAAA GTGTTGGTGAAGATGACCGCTCAGGAGCTCTCCAATGGAATTTCAGACTCGGGGCATCTCTATGCAGCCC TCAGAGCAAGCAAGACACTGACACCTTCAGGGGACTTGCAGGAGACCTTCAGTGGGATGGATCAGGTGAA GGTGATGAAAAGAATTGCAGAGATGACAGACATCAAGCCCATCCTGAGAAAACTGCCCCGGATCAAGAAG TATCTACTAAACTGTGACAACATGAGATGCTCAGTGAATGCCACCCCTCAGCAGATGCCTCAGGCAGAAA AAGAGGTGGAAAACTTCCTTAGAAATGTTGGCCGAAGCAAAAAGGAACGGAAGCCTGTCCGCCCGCATAT TGTCGAGAAACCCACACCCAGTGGCCCCAGTGGAGCTGCACATGTCAGTGGGTCCCAGATCGTCAGAAAA TTGGTGACAGACCCCACCTTCAAACCCTGCCAGATGAAGACACATTTTGTGCTGCCCTTCCCTGTGAATT ACATTGGCGAGTGTGTCAGGACTGTCCCGTATGCTGATCCAGACCATGCCAGCCTTAAGATCCTTGCCCG TCTAATGACAGCTAAATTCTTGCATACGGAAATTCGAGAGAAGGGGGGTGCTTATGGTGGCGGTGCTAAA CTCACCCACAGTGGGATTTTCACGCTTTACTCTTACAGGGATCCCAATTCCATAGAAACACTCCAGTCTT TTGGGAAAGCTGTAGACTGGGCTAAGTCTGGAAAGTTCACACAGCAGGACATTGATGAAGCCAAGCTGTC TGTTTTCTCTACTGTGGATTCTCCTGTTGCTCCATCCGATAAAGGAATGGACCACTTCTTGTATGGCCTC TCCGATGAGATGAAGCAGGCATACCGAGAACAGCTCTTTGCTGTCAACCACGACAAACTGACCTCTGTGA GCCATAAATACCTTGGCATCGGGAAGAGCACACACGGCCTGGCTATCCTCGGACCAGAGAACTCAAAAAT TGCCAAAGACCCATCATGGATCATAAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220510 representing NM_145131
Red=Cloning site Green=Tags(s) MWRFSGRRGLCAVQRLSCGRVHHRVWREKSDQACERALQYKVGEKIHGFTVNQVTPVPELFLTAVKLSHD NTGARYLHLAREDKNNLFSVQFRTTPMDSTGVPHVLEHTVLCGSQKYPCRDPFFKMLNRSLSTFMNAMTA SDYTIYPFSTQNPKDFQNLLSVYLDATFFPCLRELDFWQEGWRLEHENPRDPQTPLIFKGVVFNEMKGAF TDNERIFSQHLQNKLLPDHTYSVVSGGDPLCIPELTWEQLKQFHATHYHPSNARFFTYGNFQLEGHLKQI HEEALSKFQRLEQSTAVPAQPHWDKPREFHITCGPDSLATETAKQTTVSVSFLLPDITDTFEAFTLSLLS SLLIAGPNSPFYKALIESGLGTDFSPDVGYNGYTREAYFSVGLQGIAEKDVKTVRELVDRTIEEVIEKGF EDDRIEALLHKIEIQTKHQSASFGLTLTSYIASCWNHDGDPVELLQIGSQLTRFRKCLKENPKFLQEKVE QYFKNNQHKLTLSMKPDDKYYEKQTQMETEKLEQKVNSLSPADKQQIYEKGLELQTQQSKHQDASCLPAL KVSDIEPSMPFTKLDIGLAAGDIPVQYCPQPTNGMVYFRAFSSLNTLPEDLRPIVPLFCSVLTKLGCGIL NYREQAQQIELKTGGMSVTPHVLPDDSQLDTYEQGVLFSSLCLERNLPDMMHLWSEIFNNPCFEEEEHFK VLVKMTAQELSNGISDSGHLYAALRASKTLTPSGDLQETFSGMDQVKVMKRIAEMTDIKPILRKLPRIKK YLLNCDNMRCSVNATPQQMPQAEKEVENFLRNVGRSKKERKPVRPHIVEKPTPSGPSGAAHVSGSQIVRK LVTDPTFKPCQMKTHFVLPFPVNYIGECVRTVPYADPDHASLKILARLMTAKFLHTEIREKGGAYGGGAK LTHSGIFTLYSYRDPNSIETLQSFGKAVDWAKSGKFTQQDIDEAKLSVFSTVDSPVAPSDKGMDHFLYGL SDEMKQAYREQLFAVNHDKLTSVSHKYLGIGKSTHGLAILGPENSKIAKDPSWIIK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145131 |
| ORF Size | 3108 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145131.1, NP_660113.1 |
| RefSeq Size | 3547 bp |
| RefSeq ORF | 3111 bp |
| Locus ID | 69617 |
| UniProt ID | Q8K411 |
| Gene Summary | Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing. Has an ATP-independent activity. Specifically cleaves peptides in the range of 5 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. Degrades the transit peptides of mitochondrial proteins after their cleavage. Also degrades other unstructured peptides. It is also able to degrade amyloid-beta protein 40, one of the peptides produced by APP processing, when it accumulates in mitochondrion. It is a highly efficient protease, at least toward amyloid-beta protein 40. Cleaves that peptide at a specific position and is probably not processive, releasing digested peptides intermediates that can be further cleaved subsequently.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223280 | Pitrm1 (untagged) - Mouse pitrilysin metallepetidase 1 (Pitrm1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 13,300.00 |
|
| MR220510 | Pitrm1 (Myc-DDK-tagged) - Mouse pitrilysin metallepetidase 1 (Pitrm1), nuclear gene encoding mitochondrial protein |
CNY 7,216.00 |
|
| MR220510L3 | Lenti ORF clone of Pitrm1 (Myc-DDK-tagged) - Mouse pitrilysin metallepetidase 1 (Pitrm1), nuclear gene encoding mitochondrial protein |
CNY 11,020.00 |
|
| MR220510L4 | Lenti ORF clone of Pitrm1 (mGFP-tagged) - Mouse pitrilysin metallepetidase 1 (Pitrm1), nuclear gene encoding mitochondrial protein |
CNY 11,020.00 |
