Il18rap (NM_010553) Mouse Tagged ORF Clone
CAT#: MG220585
- TrueORF®
Il18rap (tGFP-tagged) - Mouse interleukin 18 receptor accessory protein (Il18rap), (10ug)
ORF Plasmid: DDK
"NM_010553" in other vectors (4)
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CNY 8,456.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AcPL; IL-1RAcPL; IL-18R-beta; IL-18RAcP; IL-18Rbeta |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG220585 representing NM_010553
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTGTTTGGGCTGGGTGTTTCTTTGGTTTGTTGCAGGAGAGAAGACCACAGGATTTAATCATTCAG CTTGTGCCACCAAAAAACTTCTGTGGACATATTCTGCAAGGGGTGCAGAGAATTTTGTCCTATTTTGTGA CTTACAAGAGCTTCAGGAGCAAAAATTCTCCCATGCAAGTCAACTGTCACCAACACAAAGTCCTGCTCAC AAACCTTGCAGTGGCAGTCAGAAGGACCTATCTGATGTCCAGTGGTACATGCAACCTCGGAGTGGAAGTC CACTAGAGGAGATCAGTAGAAACTCTCCCCATATGCAGAGTGAAGGCATGCTGCATATATTGGCCCCACA GACGAACAGCATTTGGTCATATATTTGTAGACCCAGAATTAGGAGCCCCCAGGATATGGCCTGTTGTATC AAGACAGTCTTAGAAGTTAAGCCTCAGAGAAACGTGTCCTGTGGGAACACAGCACAAGATGAACAAGTCC TACTTCTTGGCAGTACTGGCTCCATTCATTGTCCCAGTCTCAGCTGCCAAAGTGATGTACAGAGTCCAGA GATGACCTGGTACAAGGATGGAAGACTACTTCCTGAGCACAAGAAAAATCCAATTGAGATGGCAGATATT TATGTTTTTAATCAAGGCTTGTATGTATGTGATTACACACAGTCAGATAATGTGAGTTCCTGGACAGTCC GAGCTGTGGTTAAAGTGAGAACCATTGGTAAGGACATCAATGTGAAGCCGGAAATTCTGGATCCCATTAC AGATACACTGGACGTAGAGCTTGGAAAGCCTTTAACTCTCCCCTGCAGAGTACAGTTTGGCTTCCAAAGA CTTTCAAAGCCTGTGATAAAGTGGTATGTCAAAGAATCTACACAGGAGTGGGAAATGTCAGTATTTGAGG AGAAAAGAATTCAATCCACTTTCAAGAATGAAGTCATTGAACGTACCATCTTCTTGAGAGAAGTTACCCA GAGAGATCTCAGCAGAAAGTTTGTTTGCTTTGCCCAGAACTCCATTGGGAACACAACACGGACCATACGG CTGAGGAAGAAGGAAGAGGTGGTGTTTGTATACATCCTTCTCGGCACGGCCTTGATGCTGGTGGGCGTTC TGGTGGCAGCTGCTTTCCTCTACTGGTACTGGATTGAAGTTGTCCTGCTCTGTCGAACCTACAAGAACAA AGATGAGACTCTGGGGGATAAGAAGGAATTCGATGCATTTGTATCCTACTCGAATTGGAGCTCTCCTGAG ACTGACGCCGTGGGATCTCTGAGTGAGGAACACCTGGCTCTGAATCTTTTCCCGGAAGTGCTAGAAGACA CCTATGGGTACAGATTGTGTTTGCTTGACCGAGATGTGACCCCAGGAGGAGTGTATGCAGATGACATTGT GAGCATCATTAAGAAAAGCCGAAGAGGAATATTTATCCTGAGTCCCAGCTACCTCAATGGACCCCGTGTC TTTGAGCTACAAGCAGCAGTGAATCTTGCCTTGGTTGATCAGACACTGAAGTTGATTTTAATTAAGTTCT GTTCCTTCCAAGAGCCAGAATCTCTTCCTTACCTTGTCAAAAAGGCTCTGCGGGTTCTCCCCACAGTCAC ATGGAAAGGCTTGAAGTCGGTCCACGCCAGTTCCAGGTTCTGGACCCAAATTCGTTACCACATGCCTGTG AAGAACTCCAACAGGTTTATGTTCAACGGGCTCAGAATTTTCCTGAAGGGCTTTTCCCCTGAAAAGGACC TAGTGACACAGAAACCCCTGGAAGGAATGCCCAAGTCTGGGAATGACCACGGAGCTCAGAACCTCCTTCT CTACAGTGACCAGAAGAGGTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220585 representing NM_010553
Red=Cloning site Green=Tags(s) MLCLGWVFLWFVAGEKTTGFNHSACATKKLLWTYSARGAENFVLFCDLQELQEQKFSHASQLSPTQSPAH KPCSGSQKDLSDVQWYMQPRSGSPLEEISRNSPHMQSEGMLHILAPQTNSIWSYICRPRIRSPQDMACCI KTVLEVKPQRNVSCGNTAQDEQVLLLGSTGSIHCPSLSCQSDVQSPEMTWYKDGRLLPEHKKNPIEMADI YVFNQGLYVCDYTQSDNVSSWTVRAVVKVRTIGKDINVKPEILDPITDTLDVELGKPLTLPCRVQFGFQR LSKPVIKWYVKESTQEWEMSVFEEKRIQSTFKNEVIERTIFLREVTQRDLSRKFVCFAQNSIGNTTRTIR LRKKEEVVFVYILLGTALMLVGVLVAAAFLYWYWIEVVLLCRTYKNKDETLGDKKEFDAFVSYSNWSSPE TDAVGSLSEEHLALNLFPEVLEDTYGYRLCLLDRDVTPGGVYADDIVSIIKKSRRGIFILSPSYLNGPRV FELQAAVNLALVDQTLKLILIKFCSFQEPESLPYLVKKALRVLPTVTWKGLKSVHASSRFWTQIRYHMPV KNSNRFMFNGLRIFLKGFSPEKDLVTQKPLEGMPKSGNDHGAQNLLLYSDQKRC TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010553 |
ORF Size | 1842 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010553.4, NP_034683.1 |
RefSeq Size | 2496 bp |
RefSeq ORF | 1845 bp |
Locus ID | 16174 |
UniProt ID | Q9Z2B1 |
Gene Summary | Interleukin-18 (or interferon-gamma inducing factor) is a proinflammatory cytokine that induces cell-mediated immunity following microbial infection. This gene encodes a member of the interleukin-1 receptor family. The encoded protein is an accessory subunit of the receptor for interleukin-18 and mediates signaling through this cytokine. Mice lacking this gene exhibit a defective cell-mediated immune response. [provided by RefSeq, Jan 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219701 | Il18rap (untagged) - Mouse interleukin 18 receptor accessory protein (Il18rap), (10ug) |
CNY 7,328.00 |
|
MR220585 | Il18rap (Myc-DDK-tagged) - Mouse interleukin 18 receptor accessory protein (Il18rap) |
CNY 6,856.00 |
|
MR220585L3 | Lenti ORF clone of Il18rap (Myc-DDK-tagged) - Mouse interleukin 18 receptor accessory protein (Il18rap) |
CNY 7,980.00 |
|
MR220585L4 | Lenti ORF clone of Il18rap (mGFP-tagged) - Mouse interleukin 18 receptor accessory protein (Il18rap) |
CNY 9,256.00 |
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