Ralgds (NM_001145836) Mouse Tagged ORF Clone
CAT#: MG221064
- TrueORF®
Ralgds (tGFP-tagged) - Mouse ral guanine nucleotide dissociation stimulator (Ralgds) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001145836" in other vectors (3)
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CNY 9,790.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Gnds; mKIAA1308; Rgds |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG221064 representing NM_001145836
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAGCGCATGTGGGCTGAGGCGGCCGGGCCCATAGGGGGCACCGAGCCGTTGTTTCCCGGTTCCC GGCGCAGCCGCAGCGTGTGGGACGCCGTGCGCCTGGAGGTGGGCGTCCCCGACAGCTGCCCAGTGGTGCT GCACAGCTTCACGCAGCTCGACCCTGATCTGCCGCGCCTGGAGAGCTCCACGCAGGAGATTGGGGAGGAG CTGATCAACGGGGTCATCTACTCCATCTCCCTGCGCAAGGTCCAGCTACACCAAGGAGCCACTAAGGGCC AGCGCTGGCTAGGGTGTGAGAACGAGTCGGCTCTGAACCTCTATGAGACCTGCAAGGTGCGCACGGTGAA GGCTGGTACTCTGGAGAAGCTGGTGGAACACCTGGTGCCTGCCTTCCAGGGCAGTGACCTTTCCTACGTC ACTGTCTTCCTGTGCACCTACAGAGCCTTCACTACCACCCAGCAGGTGCTAGACCTGCTGTTCAAAAGAT ATGGCTGCATCCTCCCCTACTCCAGTGAGGACGGCGGACCGCAGGACCAACTCAAAAATGCCATCTCCTC CATCCTGGGCACCTGGCTGGACCAATACTCAGAGGATTTCTGTCAACCTCCGGACTTTCCCTGCCTCAAG CAGCTGGTGGCTTATGTACAGCTCAACATGCCTGGCTCAGATCTGGAGCGCCGCGCTCACCTTCTCCTGG CCCAGCTGGAGGACCTGGAGCCCAGTGAGGCTGAGTCTGAGGCCCTGTCCCCAGCTCCAGTGCTGTCTCT GAAGCCAGCTTCACAGCTAGAACCTGCACTGCTGCTGACGCCCAGCCAAGTGGTGACATCAACTCCAGTA CGAGAGCCCGCTGCGGCCCCAGTGCCAGTGCTGGCCTCCAGCCCAGTGGTGGCACCAGCTCCTGAGCTAG AACCAGTTCCAGAGCCACCTCAAGAGCCTGAGCCATCCCTAGCACTGGCTCCAGAGCTGGAGCCCGCCGT CTCACAGAGCCTGGAACTGGAGTCAGCTCCTGTGCCCACTCCTGCCTTAGAGCCTTCCTGGTCTCTGCCT GAAGCCACGGAGAATGGACTAACCGAGAAGCCTCACCTTCTGCTGTTCCCTCCTGACTTGGTGGCTGAAC AGTTTACTCTGATGGATGCAGAACTATTCAAGAAAGTCGTGCCCTACCACTGCCTGGGCTCCATCTGGTC CCAACGGGACAAGAAGGGCAAGGAGCACCTCGCGCCTACCATCCGCGCCACTGTCGCCCAGTTCAACAAC GTGGCCAACTGTGTCATTACTACCTGCCTTGGGGACCAGAGTATGAAGGCTCCGGACAGGGCCCGGGTGG TGGAACACTGGATCGAGGTGGCCAGGGAGTGCAGAGCGCTCAAGAATTTCTCCTCCCTCTACGCCATCCT CTCTGCTCTACAGAGCAATGCCATCCACCGCCTAAAGAAGACGTGGGAAGAGGTCTCCAGGGACAGCTTT CGAGTGTTCCAGAAACTGTCGGAGATCTTCTCTGATGAGAACAACTACTCCCTGAGCAGAGAGCTGCTCA TCAAGGAAGGAACCTCCAAGTTTGCCACACTGGAGATGAACCCTAGGAGAGCCCAGAGGCGGCAGAAGGA GACAGGAGTCATCCAGGGCACCGTTCCCTACCTGGGCACATTCCTCACTGACCTGGTGATGCTGGACACT GCCATGAAGGACTATCTCTATGGGAGACTGATCAACTTTGAAAAGAGAAGGAAGGAGTTCGAAGTCATTG CCCAGATCAAGTTGCTACAGTCAGCCTGCAACAACTACAGCATTGCTCCGGAAGAACACTTTGGAACATG GTTCCGAGCTATGGAGCGACTCAGTGAGGCTGAGAGCTACACCCTGTCGTGTGAGCTGGAGCCCCCGTCT GAGTCGGCCAGCAACACCCTGAGGAGCAAGAAAAGCACAGCCATTGTCAAGCGCTGGAGCGACCGCCAGG CTCCCAGCACGGAGCTCAGCACCAGTAGCAGTGCCCACTCCAAGTCCTGTGACCAGCTTCGGTGCAGCCC TTACCTCGGCAGCGGGGACATCACCGACGCGCTCAGTGTGCACTCAGCTGGCTCTTCCAGCTCTGATGTG GAGGAGATCAACATGAGCTTCGTCCCAGAGTCTCCTGATGGCCAGGAAAAGAAGTTCTGGGAGTCAGCCT CCCAGTCGTCCCCAGAGACCTCTGGCATCAGCTCCGCCTCCAGCAGCACCTCCTCTTCGTCAGCCTCCAC CACGCCCGTGTCTACCACGCGCACCCACAAGCGCTCCGTCTCAGGGGTCTGCAGCTACAGCTCCTCACTG CCTCTCTACAACCAGCAGGTGGGCGACTGCTGCATCATCAGGGTCAGCCTGGATGTGGACAACGGCAACA TGTACAAGAGCATCCTGGTGACCAGCCAGGATAAGGCTCCGACTGTCATCCGAAAAGCCATGGACAAACA CAACCTAGATGAGGACGAGCCGGAGGATTATGAGCTGGTGCAGATCATCTCAGAGGATCACAAGCTGAAG ATTCCAGAAAACGCCAATGTGTTCTATGCCATGAACTCTACCGCCAACTATGACTTCATCCTAAAGAAGC GGACCTTCACTAAGGGGGCTAAAGTCAAGCATGGAGCCAGCTCCACCCTCCCTCGTATGAAGCAGAAGGG ACTCAGGATTGCCAAAGGCATCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221064 representing NM_001145836
Red=Cloning site Green=Tags(s) MVQRMWAEAAGPIGGTEPLFPGSRRSRSVWDAVRLEVGVPDSCPVVLHSFTQLDPDLPRLESSTQEIGEE LINGVIYSISLRKVQLHQGATKGQRWLGCENESALNLYETCKVRTVKAGTLEKLVEHLVPAFQGSDLSYV TVFLCTYRAFTTTQQVLDLLFKRYGCILPYSSEDGGPQDQLKNAISSILGTWLDQYSEDFCQPPDFPCLK QLVAYVQLNMPGSDLERRAHLLLAQLEDLEPSEAESEALSPAPVLSLKPASQLEPALLLTPSQVVTSTPV REPAAAPVPVLASSPVVAPAPELEPVPEPPQEPEPSLALAPELEPAVSQSLELESAPVPTPALEPSWSLP EATENGLTEKPHLLLFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTIRATVAQFNN VANCVITTCLGDQSMKAPDRARVVEHWIEVARECRALKNFSSLYAILSALQSNAIHRLKKTWEEVSRDSF RVFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPRRAQRRQKETGVIQGTVPYLGTFLTDLVMLDT AMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPEEHFGTWFRAMERLSEAESYTLSCELEPPS ESASNTLRSKKSTAIVKRWSDRQAPSTELSTSSSAHSKSCDQLRCSPYLGSGDITDALSVHSAGSSSSDV EEINMSFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVSTTRTHKRSVSGVCSYSSSL PLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPTVIRKAMDKHNLDEDEPEDYELVQIISEDHKLK IPENANVFYAMNSTANYDFILKKRTFTKGAKVKHGASSTLPRMKQKGLRIAKGIF TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001145836 |
ORF Size | 2685 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001145836.1, NP_001139308.1 |
RefSeq Size | 3617 bp |
RefSeq ORF | 2688 bp |
Locus ID | 19730 |
Gene Summary | Stimulates the dissociation of GDP from the Ras-related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. Interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR221064 | Ralgds (Myc-DDK-tagged) - Mouse ral guanine nucleotide dissociation stimulator (Ralgds), transcript variant 2 |
CNY 8,930.00 |
|
MR221064L3 | Lenti ORF clone of Ralgds (Myc-DDK-tagged) - Mouse ral guanine nucleotide dissociation stimulator (Ralgds), transcript variant 2 |
CNY 10,830.00 |
|
MR221064L4 | Lenti ORF clone of Ralgds (mGFP-tagged) - Mouse ral guanine nucleotide dissociation stimulator (Ralgds), transcript variant 2 |
CNY 10,830.00 |