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Cntfr (NM_001146080) Mouse Tagged ORF Clone
CAT＃: MG221101
- TrueORF®
Cntfr (tGFP-tagged) - Mouse ciliary neurotrophic factor receptor (Cntfr) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_001146080" in other vectors (3)
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CNY 2,090.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Cntfralpha |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221101 representing NM_001146080
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTTCTGTCCCATGGGCCTGCTGTGCTGTGCTTGCCGCTGCCGCCGCCGCTGTCTACACGCAGA AACACAGTCCACAGGAGGCACCCCACGTGCAGTATGAGCGTCTGGGCGCAGATGTGACGCTGCCGTGTGG GACAGCGAGCTGGGACGCAGCTGTGACATGGAGGGTAAACGGGACAGATCTGGCCCCTGACCTGCTCAAC GGCTCTCAGCTGATACTGCGAAGCTTAGAACTGGGCCACAGTGGCCTATACGCCTGTTTCCACCGTGACT CCTGGCACCTGCGCCACCAAGTCCTTCTACATGTGGGTTTGGCTGGGCTTCAGTCTCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221101 representing NM_001146080
Red=Cloning site Green=Tags(s) MAASVPWACCAVLAAAAAAVYTQKHSPQEAPHVQYERLGADVTLPCGTASWDAAVTWRVNGTDLAPDLLN GSQLILRSLELGHSGLYACFHRDSWHLRHQVLLHVGLAGLQSP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001146080 |
| ORF Size | 339 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146080.1, NP_001139552.1 |
| RefSeq Size | 678 bp |
| RefSeq ORF | 342 bp |
| Locus ID | 12804 |
| Gene Summary | This gene encodes the alpha subunit of the ciliary neurotrophic factor (CNTF) receptor that triggers the assembly of a trimolecular complex upon binding to CNTF, and initiate a downstream signaling process. The encoded preproprotein undergoes proteolytic processing to generate a glycosylphosphatidylinositol-linked cell surface protein. Mice lacking the encoded protein die shortly after birth and exhibit a reduction of motoneuron number at birth. The transgenic disruption of this gene specifically in the skeletal muscle followed by a peripheral nerve lesion impairs motor neuron axonal regeneration across the lesion site. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR221101 | Cntfr (Myc-DDK-tagged) - Mouse ciliary neurotrophic factor receptor (Cntfr), transcript variant 3 |
CNY 1,900.00 |
|
| MR221101L3 | Lenti ORF clone of Cntfr (Myc-DDK-tagged) - Mouse ciliary neurotrophic factor receptor (Cntfr), transcript variant 3 |
CNY 3,800.00 |
|
| MR221101L4 | Lenti ORF clone of Cntfr (mGFP-tagged) - Mouse ciliary neurotrophic factor receptor (Cntfr), transcript variant 3 |
CNY 3,800.00 |
