United States
Germany
Japan
United Kingdom
China
Kcnk4 (NM_008431) Mouse Tagged ORF Clone
CAT＃: MG221486
- TrueORF®
Kcnk4 (tGFP-tagged) - Mouse potassium channel subfamily K member 4 (Kcnk4), (10ug)
ORF Plasmid: DDK
"NM_008431" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MLZ-622; Tex40; TRAAK; TRAAKt |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221486 representing NM_008431
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCAGCACCACACTCCTGGCTCTGCTGGCACTGGTGCTGCTTTACTTGGTATCTGGGGCTCTAGTGT TCCAGGCTCTGGAGCAGCCTCACGAGCAGCAGGCTCAGAAGAAAATGGATCATGGCCGAGACCAGTTTCT GAGGGACCATCCCTGTGTGAGCCAGAAGAGCCTGGAGGATTTCATCAAGCTCCTGGTTGAAGCCCTGGGA GGGGGCGCAAACCCAGAAACCAGCTGGACCAATAGCAGCAACCACTCATCAGCTTGGAACCTGGGCAGCG CCTTCTTTTTCTCGGGGACCATCATCACTACCATCGGCTATGGCAATATAGTCTTACACACAGATGCCGG GCGTCTCTTTTGTATCTTCTATGCACTGGTGGGGATCCCACTGTTCGGGATGCTGCTGGCGGGAGTCGGG GACCGGCTGGGCTCCTCTCTGCGCCGGGGCATCGGCCACATCGAAGCAATCTTCTTGAAGTGGCATGTGC CACCGGGGCTGGTGAGAAGTCTGTCCGCAGTGCTCTTCCTGCTGATCGGCTGCCTGCTCTTTGTCCTCAC TCCTACCTTCGTGTTCTCCTACATGGAGAGCTGGAGCAAGTTAGAAGCCATCTACTTTGTTATAGTGACT CTCACCACTGTAGGCTTTGGCGATTATGTACCCGGCGATGGCACCGGGCAGAACTCTCCAGCCTACCAGC CGCTGGTGTGGTTCTGGATCTTGTTTGGCCTAGCCTACTTCGCCTCAGTGCTCACCACCATCGGCAACTG GTTGCGAGCAGTGTCCCGCCGAACTCGGGCAGAGATGGGTGGCCTAACGGCACAGGCTGCTAGCTGGACC GGCACAGTGACAGCGCGAGTGACCCAGCGAACTGGGCCCAGCGCCCCGCCGCCAGAGAAGGAGCAACCAC TCCTGCCCTCCTCTTTGCCGGCACCGCCTGCTGTTGTTGAGCCAGCCGGCAGGCCCGGCTCCCCTGCACC CGCAGAGAAGGTTGAGACTCCGTCCCCGCCCACGGCCTCAGCTCTGGATTACCCCAGTGAGAATCTGGCC TTCATCGACGAGTCCTCAGACACGCAGAGTGAGCGTGGCTGTGCCCTGCCTCGGGCTCCTCGGGGTCGCC GCCGACCCAACCCATCCAAAAAGCCTTCCAGACCCCGGGGTCCTGGGCGACTCCGAGACAAGGCCGTGCC GGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221486 representing NM_008431
Red=Cloning site Green=Tags(s) MRSTTLLALLALVLLYLVSGALVFQALEQPHEQQAQKKMDHGRDQFLRDHPCVSQKSLEDFIKLLVEALG GGANPETSWTNSSNHSSAWNLGSAFFFSGTIITTIGYGNIVLHTDAGRLFCIFYALVGIPLFGMLLAGVG DRLGSSLRRGIGHIEAIFLKWHVPPGLVRSLSAVLFLLIGCLLFVLTPTFVFSYMESWSKLEAIYFVIVT LTTVGFGDYVPGDGTGQNSPAYQPLVWFWILFGLAYFASVLTTIGNWLRAVSRRTRAEMGGLTAQAASWT GTVTARVTQRTGPSAPPPEKEQPLLPSSLPAPPAVVEPAGRPGSPAPAEKVETPSPPTASALDYPSENLA FIDESSDTQSERGCALPRAPRGRRRPNPSKKPSRPRGPGRLRDKAVPV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008431 |
| ORF Size | 1194 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008431.3 |
| RefSeq Size | 1757 bp |
| RefSeq ORF | 1197 bp |
| Locus ID | 16528 |
| UniProt ID | O88454 |
| Gene Summary | Voltage-insensitive potassium channel (PubMed:9628867). Channel opening is triggered by mechanical forces that deform the membrane. Channel opening is triggered by raising the intracellular pH to basic levels (By similarity). The channel is inactive at 24 degrees Celsius (in vitro); raising the temperature to 37 degrees Celsius increases the frequency of channel opening, with a further increase in channel activity when the temperature is raised to 42 degrees Celsius (By similarity). Plays a role in the sensory perception of pain caused by pressure (PubMed:19279663). Plays a role in the perception of pain caused by heat (PubMed:19279663).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208813 | Kcnk4 (untagged) - Mouse potassium channel, subfamily K, member 4 (Kcnk4), (10ug) |
CNY 3,990.00 |
|
| MR221486 | Kcnk4 (Myc-DDK-tagged) - Mouse potassium channel, subfamily K, member 4 (Kcnk4) |
CNY 3,656.00 |
|
| MR221486L3 | Lenti ORF clone of Kcnk4 (Myc-DDK-tagged) - Mouse potassium channel, subfamily K, member 4 (Kcnk4) |
CNY 4,750.00 |
|
| MR221486L4 | Lenti ORF clone of Kcnk4 (mGFP-tagged) - Mouse potassium channel, subfamily K, member 4 (Kcnk4) |
CNY 4,750.00 |
