Camk1d (NM_177343) Mouse Tagged ORF Clone
CAT#: MG222155
- TrueORF®
Camk1d (tGFP-tagged) - Mouse calcium/calmodulin-dependent protein kinase ID (Camk1d), (10ug)
ORF Plasmid: DDK
"NM_177343" in other vectors (4)
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CNY 4,280.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | A630059D12Rik; CaMKIdelta; CKLiK; E030025C11Rik; mCKLiK |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222155 representing NM_177343
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGGGAGAACGGCGAGAGCAGCTCCTCCTGGAAAAAGCAAGCAGAAGACATTAAGAAGATCTTCG AGTTCAAGGAGACCCTCGGAACTGGGGCCTTTTCTGAAGTTGTTTTAGCCGAGGAGAAAGCTACTGGGAA GCTCTTCGCAGTGAAGTGCATCCCGAAGAAGGCGCTGAAGGGCAAGGAGAGCAGCATCGAGAACGAGATT GCCGTGCTTAGAAAGATTAAGCATGAAAACATTGTTGCCTTGGAAGATATTTATGAAAGCCCAAATCACC TCTACCTGGTCATGCAACTTGTGTCTGGTGGAGAACTCTTCGATCGGATAGTGGAGAAGGGGTTTTACAC AGAGAAAGATGCCAGCACTCTCATCCGCCAGGTCCTGGATGCCGTATACTATCTCCACAGAATGGGCATT GTCCACAGGGACCTCAAGCCGGAGAATCTCTTATACTACAGTCAAGACGAGGAGTCCAAAATAATGATCA GTGACTTTGGCTTGTCGAAAATGGAGGGCAAAGGAGATGTGATGTCCACGGCCTGCGGGACCCCAGGCTA TGTTGCTCCGGAAGTTCTCGCCCAGAAACCGTACAGCAAAGCTGTGGACTGCTGGTCCATCGGGGTGATC GCCTATATCTTGCTCTGTGGTTACCCTCCTTTTTATGATGAAAATGACTCGAAGCTGTTTGAACAGATCC TCAAGGCAGAATATGAGTTTGATTCCCCCTACTGGGATGACATCTCCGACTCTGCCAAAGACTTCATTCG GAATCTGATGGAGAAAGACCCAAATAAAAGATACACTTGTGAGCAGGCAGCTCGACACCCATGGATTGCT GGTGACACAGCCCTTAGCAAAAACATTCACGAATCTGTCAGTGCCCAGATCCGGAAGAATTTTGCAAAGA GCAAATGGAGACAAGCGTTTAACGCCACGGCAGTCGTGAGACATATGCGGAGGCTCCAGCTTGGCAGCAG CCTGGACAGTTCAAATGCAAGTGTCTCAAGCAACCTCAGTTTGGCCAGCCAAAAAGATTGTCTGGCACCT TCCACGCTCTGTAGTTTCCTTTCTTCTTCGTCGGGGGTCGCAGGAGTCGGAGCTGAGAGGAGACCCAGGC CCACCACTGTGACAACAGGGCACACTGGAAGCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222155 representing NM_177343
Red=Cloning site Green=Tags(s) MARENGESSSSWKKQAEDIKKIFEFKETLGTGAFSEVVLAEEKATGKLFAVKCIPKKALKGKESSIENEI AVLRKIKHENIVALEDIYESPNHLYLVMQLVSGGELFDRIVEKGFYTEKDASTLIRQVLDAVYYLHRMGI VHRDLKPENLLYYSQDEESKIMISDFGLSKMEGKGDVMSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVI AYILLCGYPPFYDENDSKLFEQILKAEYEFDSPYWDDISDSAKDFIRNLMEKDPNKRYTCEQAARHPWIA GDTALSKNIHESVSAQIRKNFAKSKWRQAFNATAVVRHMRRLQLGSSLDSSNASVSSNLSLASQKDCLAP STLCSFLSSSSGVAGVGAERRPRPTTVTTGHTGSK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_177343 |
ORF Size | 1155 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177343.4, NP_796317.2 |
RefSeq Size | 7128 bp |
RefSeq ORF | 1158 bp |
Locus ID | 227541 |
UniProt ID | Q8BW96 |
Gene Summary | Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, activates CREB-dependent gene transcription, regulates calcium-mediated granulocyte function and respiratory burst and promotes basal dendritic growth of hippocampal neurons. In neutrophil cells, required for cytokine-induced proliferative responses and activation of the respiratory burst. Activates the transcription factor CREB1 in hippocampal neuron nuclei. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta (By similarity). Isoform 1 but not isoform 2 activates CREB1.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212802 | Camk1d (untagged) - Mouse calcium/calmodulin-dependent protein kinase ID (Camk1d), (10ug) |
CNY 3,990.00 |
|
MR222155 | Camk1d (Myc-DDK-tagged) - Mouse calcium/calmodulin-dependent protein kinase ID (Camk1d) |
CNY 3,900.00 |
|
MR222155L3 | Lenti ORF clone of Camk1d (Myc-DDK-tagged) - Mouse calcium/calmodulin-dependent protein kinase ID (Camk1d) |
CNY 5,800.00 |
|
MR222155L4 | Lenti ORF clone of Camk1d (mGFP-tagged) - Mouse calcium/calmodulin-dependent protein kinase ID (Camk1d) |
CNY 5,800.00 |