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Dbf4 (NM_013726) Mouse Tagged ORF Clone
CAT＃: MG222369
- TrueORF®
Dbf4 (tGFP-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_013726" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA545217; Ask |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222369 representing NM_013726
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCTCGAGACCATGAGGATCCACAGCAAAGCACCTCTCCCGGGTGGCATCCAAGACAGAAATGAAA AGAATCGACCATCCTTGAAATCCCTGAAGGCTGATAATAGACTGGAAAAATCCAAATATAAGCCCCTTTG GGGAAAAATATTTTACCTTGACTTGCCTTCTATCACCATATGTGAGAAACTACAAAAAGATATAAAGGAA CTCGGAGGGCGAGTTGAAGAATTTCTCAGCAAAGATATCAGTTATTTTGTTTCAAATAAAAAGGAAGCAA AGTATGCACAGACATTGGGTCGCGTTTCTCCTGTACCAAGTCCAGAATCTGCATATACTGCAGAAACCAC CTCTCCTCATCCCAGCCATGATGGAAGTTCCTTTAAGTCTCAAGACAGAGTATGTTTGAGCCGAGGAAAA TTGTTAGCTGAAAAAGCTGTGAAGGATCATGATTTTATTCCTGCTAATAGTATATTATCAAATGCCTTAT CATGGGGAGTAAAGATTCTTCATATTGATGACATTCGATACTACATTGAACAGAAGAAGAAAGCACTGAG TGCACTCAAGAAATCCAGTGCTTCTGGGAAGGATGCGGGGAAAAAAGCTGGTCCTGGAATACAAAAGACA AGAACAGGGAGACTCAAGAAGCCTTTTTTAAAGGTTGAAGATGTGAACCGGTGCTACAGGCCATTTTACC TTCAGCTCCCCAGTCTGCCGTGTATAAACTACTTTCTTCAGAAGCCCTGCAGTCCGTTTGATATAGAGAA GTCATCCAGCGTCCAGAAACAAGCTCAGCCCAAGCTCAGAATTAATATGGACGGCGATAAATGTGGAACC CCAGTTCAACTCCAGTTGAAGGAAAAGAGAAAAAAAGGATATTGTGAATGTTGCTTACAGAAATACGAAG ATCTCGAAACTCACCTTCTGAGTGAGAAACACAGGAACTTTGCACAGAGTAACCAGTATCAAGTTGTTGA TGATATTGTATCTCAGTTAGTTTTCGATTTTGTGGAATATGGAAGAGACACACCTCAAAAGAAAAGAATA AGATACAGTGTCGGGTCCCTTTCTTCTGTTTCTGCAAATGTCCTGAAAAACACTGCACCGAAGGAGAAGC CACTCTTGGAGCCCAATTTCCAGAAGGACGTGGGCGAAAGCAGTGGGCACCTGCTCAAGCCGAACTCTCA GTATGAAGAAACCCAGAAACCTGAAGAAAAGCATGGGTTTGCTTCAGAGCCCACAACCTATTCTTCAGCT GGATTGAAAGGATGTGACAGGAAACCAGTGAGCATGTTCAATGCAAGTGAGCCTGACCCAGAGCAGGAGT ATGCACAGCTACCTCTGCGTGACAGCACACCGGAACACCAAGTGACCGAGGGTAGAAATGACGGAGAACA GAGAGTAGACCCTGCTCCAGGCGTGTCGCAGTCATGTGGACAAGTTTCTCATCTCAGTACAGAAAGTAAT CTACCCCAACCACAGTTAGCTGCAGACATTACACAGCTTTCAGCAAAAGATCTGCAAGAAAAGGGCTTTC ATGTTGTGATAGGTCATGCTTCTGACCTGGTGGCATTAAATACATCAAAAGAGCAGCTAACAATGAAGGC GAGAACTCCACCCTGTAGTCCCCAGGAGCCTCATGAGTGTGACACTGAGAACATGGAGAATTTGCCTTGT GGTAAAATCCAGCGGAAAGTGAGAATGCTATTAGGGCAACAGAAAGCAAATGCTGAACCGAGTGCTGAAT TGGATAAAAAGAGAACTGAGTATCTTCCTGCACATGAAGACAGAACTTGTGGGTCACCAGTACAGTCTCT ATTGGACCTTTTTCAGACAAGCGAGGAGAAATCAGAATTTTTGGGTTTTACAGGCTACACTGAAAACAGT GGAATATGTGATGTTTTAGATATTTGGGAGGAAGAAAATTCAAGTACCCTGTTGTCAACGTTTTTTTCTT CCCCTTCAACTTCTGCATTCGTTGGATTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222369 representing NM_013726
Red=Cloning site Green=Tags(s) MNLETMRIHSKAPLPGGIQDRNEKNRPSLKSLKADNRLEKSKYKPLWGKIFYLDLPSITICEKLQKDIKE LGGRVEEFLSKDISYFVSNKKEAKYAQTLGRVSPVPSPESAYTAETTSPHPSHDGSSFKSQDRVCLSRGK LLAEKAVKDHDFIPANSILSNALSWGVKILHIDDIRYYIEQKKKALSALKKSSASGKDAGKKAGPGIQKT RTGRLKKPFLKVEDVNRCYRPFYLQLPSLPCINYFLQKPCSPFDIEKSSSVQKQAQPKLRINMDGDKCGT PVQLQLKEKRKKGYCECCLQKYEDLETHLLSEKHRNFAQSNQYQVVDDIVSQLVFDFVEYGRDTPQKKRI RYSVGSLSSVSANVLKNTAPKEKPLLEPNFQKDVGESSGHLLKPNSQYEETQKPEEKHGFASEPTTYSSA GLKGCDRKPVSMFNASEPDPEQEYAQLPLRDSTPEHQVTEGRNDGEQRVDPAPGVSQSCGQVSHLSTESN LPQPQLAADITQLSAKDLQEKGFHVVIGHASDLVALNTSKEQLTMKARTPPCSPQEPHECDTENMENLPC GKIQRKVRMLLGQQKANAEPSAELDKKRTEYLPAHEDRTCGSPVQSLLDLFQTSEEKSEFLGFTGYTENS GICDVLDIWEEENSSTLLSTFFSSPSTSAFVGF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013726 |
| ORF Size | 1989 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013726.3, NP_038754.1 |
| RefSeq Size | 2444 bp |
| RefSeq ORF | 1992 bp |
| Locus ID | 27214 |
| UniProt ID | Q9QZ41 |
| Gene Summary | Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role DNA in replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220248 | Dbf4 (untagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1, (10ug) |
CNY 8,550.00 |
|
| MR222369 | Dbf4 (Myc-DDK-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1 |
CNY 4,936.00 |
|
| MR222369L3 | Lenti ORF clone of Dbf4 (Myc-DDK-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1 |
CNY 6,460.00 |
|
| MR222369L4 | Lenti ORF clone of Dbf4 (mGFP-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1 |
CNY 6,460.00 |
