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Tie1 (NM_011587) Mouse Tagged ORF Clone
CAT＃: MG222502
- TrueORF®
Tie1 (tGFP-tagged) - Mouse tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), (10ug)
ORF Plasmid: DDK
"NM_011587" in other vectors (4)
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CNY 10,830.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | D430008P04Rik; TIE; tie-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222502 representing NM_011587
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTGGTGGGGATCCTCTTTGCTGCTCCCCACTCTTTTCTTGGCCTCTCATGTTGGTGCGTCTGTTG ACTTAACATTGCTGGCCAACCTGCGCATCACAGACCCCCAGCGTTTCTTCCTGACCTGCGTGTCTGGTGA GGCCGGAGCAGGGAGGAGCTCGGACCCGCCCCTGCTGCTGGAGAAGGATGATCGCATCGTGCGCACCTTC CCGCCGGGGCAGCCCCTGTACCTGGCACGCAACGGTTCTCACCAGGTCACGCTGAGGGGCTTCTCCAAGC CCTCGGATTTGGTAGGCGTCTTCTCCTGTGTGGGTGGTGCTGGCGCGCGGCGCACTCGAGTTCTCTATGT GCACAACAGCCCAGGGGCACACCTGTTTCCAGACAAGGTCACACACACGGTGAACAAAGGTGACACTGCT GTGCTTTCTGCCCATGTGCACAAGGAAAAGCAGACTGACGTGATCTGGAAGAACAACGGATCCTACTTCA ATACCCTAGACTGGCAAGAGGCCGATGACGGGCGTTTTCAACTGCAGCTCCAAAATGTACAGCCACCATC AAGTGGCATCTACAGTGCCACCTACCTAGAGGCCAGCCCCCTGGGCAGTGCCTTCTTTCGGCTCATCGTG CGAGGCTGTGGGGCTGGACGCTGGGGGCCAGGATGTGTCAAGGATTGCCCGGGCTGCCTGCATGGAGGTG TCTGTCATGACCATGATGGCGAATGTGTGTGTCCCCCAGGATTCACTGGCACCCGCTGTGAGCAGGCCTG CAGGGAGGGTCGTTTTGGACAGAGCTGTCAGGAACAGTGCCCAGGCACAGCAGGTTGTAGAGGTCTCACC TTCTGCCTCCCGGATCCCTATGGCTGTTCCTGTGGATCTGGCTGGAGAGGAAGCCAGTGCCAGGAAGCAT GTGCACCTGATCATTTTGGGGCTGATTGTCGCCTCCAGTGCCAGTGTCAAAATGGTGGCACTTGTGACCG GTTCAGCGGCTGCGTCTGCCCCTCTGGGTGGCATGGAGTCCACTGTGAAAAGTCAGACCGGATCCCCCAG ATCCTCAGTATGGCCACAGAGGTGGAGTTCAACATAGGGACGATGCCCCGGATCAACTGTGCAGCTGCCG GGAATCCCTTCCCAGTGCGGGGCAGCATGAAACTTCGCAAGCCAGATGGCACCATGCTTTTGTCTACCAA AGTCATTGTGGAGCCAGACAGGACCACAGCAGAGTTTGAAGTGCCCAGTTTGACTCTTGGGGACAGTGGG TTCTGGGAATGCCGCGTATCGACTTCTGGTGGCCAAGACAGCCGGCGCTTCAAGGTCAATGTCAAAGTAC CCCCAGTGCCTTTGACTGCACCTCGACTCCTGGCCAAGCAGAGCCGTCAGCTTGTGGTCTCCCCACTGGT CTCCTTTAGTGGGGATGGACCCATCTCCTCTGTCCGCCTGCACTACCGGCCCCAGGACAGCACGATTGCC TGGTCTGCCATTGTGGTGGATCCCAGTGAGAATGTGACATTAATGAACCTGAAGCCAAAGACAGGATACA ATGTCCGAGTGCAGCTGAGCCGGCCAGGGGAAGGAGGAGAAGGAGGCTGGGGACCTTCTGCCCTTATGAC TACTGACTGTCCTGAGCCTTTGCTGCAGCCCTGGCTAGAGAGCTGGCACGTGGAGGGTCCTGACAGGCTC CGTGTGAGCTGGTCTCTACCCTCGGTGCCACTGTCCGGTGATGGTTTCCTGCTGCGCCTGTGGGATGGGG CCCGGGGACAGGAGAGGCGGGAGAACATCTCATTCCCCCAGGCCCGCACTGCCCTCCTGACTGGACTCAC GCCGGGCACCCACTACCAGCTGGATGTGCGACTGTATCACTGTACCCTCCTGGGCCCTGCCTCACCCCCT GCGCATGTGCACCTGCCCCCCAGCGGGCCTCCAGCTCCCCGCCACCTCCATGCCCAGGCCCTCTCAGACT CTGAGATTCAACTCATGTGGCAGCATCCCGAGGCTCCGTCTGGGCCTATATCCAAGTACATCGTGGAGAT TCAGGTGGCCGGGGGCTCAGGAGACCCACAGTGGATGGATGTAGACAGGCCCGAGGAGACAAGCATCATT GTTCGTGGCCTCAATGCTAGCACACGATATCTTTTCCGTGTGCGGGCCAGTGTGCAGGGTCTTGGCGACT GGAGCAATACAGTAGAAGAGGCCACTCTGGGCAATGGGCTGCAGAGTGAGGACCCAGTCCGAGAAAGCCG GGCAGCTGAAGAAGGCCTGGATCAGCAGCTGGTCCTGGCTGTGGTAGGTTCCGTCTCTGCCACCTGCCTC ACCATCCTGGCTGCCCTTTTAGCCTTGGTGTGTATCCGAAGAAGCTGCCTACATCGGAGACGCACCTTCA CTTACCAGTCAGGATCGGGTGAAGAGACCATCCTGCAGTTCAGCTCAGGGACCTTGACCTTGACCCGGCG ACCGAAGCCACAGCCTGAGCCCTTGAGTTACCCCGTGCTGGAGTGGGAGGACATCACCTTTGAGGACCTC ATAGGGGAGGGCAACTTTGGCCAAGTGATCCGGGCCATGATCAAGAAGGACGGGCTCAAGATGAACGCAG CCATCAAGATGCTAAAAGAGTATGCGTCTGAAAATGACCATCGAGACTTTGCAGGTGAACTAGAAGTTCT GTGCAAACTAGGACACCACCCCAATATTATCAACCTCTTGGGGGCCTGTGAGAACCGAGGCTACTTGTAT ATTGCTATCGAATACGCCCCCTATGGAAACCTACTGGACTTCCTGAGAAAGAGCAGGGTCCTGGAGACTG ATCCAGCTTTTGCTCGAGAGCATGGGACGGCCTCCACACTCAGCTCTCGGCAGCTGCTGCGCTTTGCCAG TGATGCAGCCAACGGCATGCAGTACCTTAGTGAGAAGCAGTTCATTCACAGGGACCTGGCTGCCCGAAAT GTGCTGGTCGGAGAGAACCTGGCCTCCAAGATTGCAGACTTTGGCCTTTCTCGAGGGGAGGAGGTGTATG TGAAGAAGACAATGGGCCGTCTCCCTGTGCGTTGGATGGCCATTGAGTCCCTCAACTACAGCGTTTATAC CACCAAGAGTGATGTCTGGTCCTTTGGGGTCCTCCTCTGGGAGATAGTGAGCCTTGGAGGCACGCCCTAC TGTGGCATGACCTGTGCTGAGCTCTACGAGAAGCTGCCTCAAGGCTATCGCATGGAGCAGCCTCGAAACT GCGATGACGAAGTGTACGAGCTGATGCGGCAGTGCTGGCGGGATCGGCCCTATGAGCGCCCCCCCTTTGC TCAGATCGCACTACAGTTGGGCCGCATGCTGGAAGCCAGGAAGGCCTACGTGAACATGTCGCTGTTTGAG AACTTCACCTATGCGGGCATCGATGCCACAGCTGAGGAGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222502 representing NM_011587
Red=Cloning site Green=Tags(s) MVWWGSSLLLPTLFLASHVGASVDLTLLANLRITDPQRFFLTCVSGEAGAGRSSDPPLLLEKDDRIVRTF PPGQPLYLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVLYVHNSPGAHLFPDKVTHTVNKGDTA VLSAHVHKEKQTDVIWKNNGSYFNTLDWQEADDGRFQLQLQNVQPPSSGIYSATYLEASPLGSAFFRLIV RGCGAGRWGPGCVKDCPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGTAGCRGLT FCLPDPYGCSCGSGWRGSQCQEACAPDHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQ ILSMATEVEFNIGTMPRINCAAAGNPFPVRGSMKLRKPDGTMLLSTKVIVEPDRTTAEFEVPSLTLGDSG FWECRVSTSGGQDSRRFKVNVKVPPVPLTAPRLLAKQSRQLVVSPLVSFSGDGPISSVRLHYRPQDSTIA WSAIVVDPSENVTLMNLKPKTGYNVRVQLSRPGEGGEGGWGPSALMTTDCPEPLLQPWLESWHVEGPDRL RVSWSLPSVPLSGDGFLLRLWDGARGQERRENISFPQARTALLTGLTPGTHYQLDVRLYHCTLLGPASPP AHVHLPPSGPPAPRHLHAQALSDSEIQLMWQHPEAPSGPISKYIVEIQVAGGSGDPQWMDVDRPEETSII VRGLNASTRYLFRVRASVQGLGDWSNTVEEATLGNGLQSEDPVRESRAAEEGLDQQLVLAVVGSVSATCL TILAALLALVCIRRSCLHRRRTFTYQSGSGEETILQFSSGTLTLTRRPKPQPEPLSYPVLEWEDITFEDL IGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACENRGYLY IAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARN VLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPY CGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFE NFTYAGIDATAEEA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011587 |
| ORF Size | 3402 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011587.1 |
| RefSeq Size | 3883 bp |
| RefSeq ORF | 3405 bp |
| Locus ID | 21846 |
| UniProt ID | Q06806 |
| Gene Summary | Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223686 | Tie1 (untagged) - Mouse tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), (10ug) |
CNY 14,540.00 |
|
| MR222502 | Tie1 (Myc-DDK-tagged) - Mouse tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1) |
CNY 7,896.00 |
|
| MR222502L3 | Lenti ORF clone of Tie1 (Myc-DDK-tagged) - Mouse tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1) |
CNY 11,780.00 |
|
| MR222502L4 | Lenti ORF clone of Tie1 (mGFP-tagged) - Mouse tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1) |
CNY 11,780.00 |
