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Adam22 (NM_001007221) Mouse Tagged ORF Clone
CAT＃: MG222779
- TrueORF®
Adam22 (tGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 22 (Adam22) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001007221" in other vectors (4)
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CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2900022I03Rik; AI854032; MDC2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222779 representing NM_001007221
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGCAGCGGCGGCCGCGTCCTTCTGGCTGCTCTGCGTCTTGGGGACCTGCCCCCTGGCGCGCTGCG GTCGGGCAGGAGTCGCCTCACTGAAAGGACTGGAGAGGGGGAAAGAAAACCGCTTCCTGGAGCGCCAGAG CATCATACCACTGCGCCTCATCTATCGCTTGGGCGGCGAGGATGAAACTCAGCACAACCAGCTCGACACG CGGGTGCGGGGCGACCCTGGCGGACCGCAGCTGACGCATGTGGACAAGGCAAGCTTCCGGGTGGACGCCT TTGGAACCTCTTTCGTTCTCGACGTCCTGCTGAACCACGAGTTGCTGTCGTCAGGTTATGTAGAGAGACA GATTGAACATGGAGGCAAGGTCGTGGAGAACAAAGGAGGAGAACACTGTTATTACCAGGGCCAGATTCGA GGAAACCCTGTCTCATTTGTTGCCCTATCAACATGCCATGGACTCCATGGGATGTTCTATGATGGAAACC ACACATATCTCATTGAGCCAGAAGAAAATGAGAAGTCTCAAGAGTCGTCTCATTGTCACTCCGTGTACAA ATCCAGACAGTTTGAATTTCCCTTGGATGACCTTCCGTCTGAATTTCAACGCGTAAACATTACTCCCCCT CAGTTTATTTTGAAGCCAAGACTAAAGAGGAGAAAAAGACAGCTTCTTCGATTTCCTCGGAATGTAGAGG AGGAAACCAAATACATCGAACTGATGATTGTGAATGATCATCTCATGTTTAAAAAGCACCGGCTTTCTGT TGTATATACTAATACCTATGCGAAGTCTGTGGTGAACATGGCAGATGTGATCTATAAAGACCAACTGAAA ACTAGGATTGTATTGGTTGCCATGGAAACCTGGGCTGCTGACAACAAGTTTGCCATATCTGAAAACCCAC TGATTACCCTTCGTGAGTTCATGAAATACAGGAGGGATTTTATCAAGGAGAAGGCTGATGCAGTTCATCT TTTCTCGGGCAGTCAATTTGAGAGTAGCCGGAGCGGGGCAGCTTATATTGGTGGGATTTGCTCGCTGCTG AGAGGAGGAGGCGTGAATGAATTTGGAAAAACGGATTTAATGGCAGTTACCCTCGCTCAGTCACTAGCCC ACAATGTCGGTATAATCTCTGACAAAAGAAAACTAGCAAGTGGTGAGTGTAAATGTGAGGACACCTGGTC CGGGTGCATAATGGGAGACACCGGCTACTATCTCCCGAAAAAGTTCACTCAGTGCAATGTCGAGGAATAT CATGACTTCCTGAATAGTGGTGGAGGTGCCTGCCTGTTCAACAAGCCTTCCAAGCTTCTTGATCCTCCTG AGTGTGGAAACGGCTTCATTGAAACCGGAGAGGAGTGTGATTGTGGCACCCCTGCAGAATGTGCCCTTGA AGGGGCAGAGTGTTGTAAGAAATGCACCTTGACCCAGGATTCTCAATGCAGTGATGGCCTTTGTTGTAAA AAGTGCAAGTTTCAGCCTCTGGGGACCGTGTGCCGAGAGGCAGTGAACGACTGTGACATTCGTGAAATAT GTTCAGGAAATTCGAGCCAGTGTGCCCCCAATGTGCATAAAATGGATGGATATTCGTGTGATGGTACTCA GGGAATTTGCTTTGGAGGAAGATGTAAAACCAGAGATAGACAATGCAAATACATCTGGGGGCAAAAGGTA ACAGCATCTGACAGGTACTGCTATGAGAAACTGAACATTGAGGGGACGGAGAAGGGTAACTGTGGGAAAG ACAAGGACACGTGGACACAGTGCAACAAACGGGATGTACTTTGTGGTTACCTCCTGTGCACAAACATTGG CAACATCCCAAGGCTCGGGGAGCTTGATGGTGAGATCACATCCACATTAGTTGTGCAGCAAGGAAGAACA TTAAACTGCAGTGGCGCGCATGTTAAACTTGAAGAAGATGTGGATCTTGGCTATGTGGAAGATGGGACGC CCTGTGGCCCTCAGATGATGTGCTTGGAACACAGGTGTCTTCCTGTGGCTTCCTTCAACTTCAGTACTTG CTCCAGCAGTAAAGCAGGCACCGTGTGCTCAGGAAATGGAGTTTGCAGCAATGAGCTCAAGTGTGTGTGC AACAGGCACTGGACAGGGGCTGACTGCGGCACCCACTTTCCTCACAATGATGATGCAAAGACTGGCATCA CCCTGTCTGGCAATGGTGTTGCTGGCACTAATATCATAATAGGAATAATTGCCGGCACCATTTTAGTGCT GGCCCTCATCTTAGGAATAACTGCCTGGGGTTATAAAAACTACCGAGAACAGAGGTCAAATGGACTCTCT CATTCTTGGAGTGAAAGGATTCCAGACACAAAACATATTTCAGACATCTGTGAAAATGGGAGACCTCGCA GTAACTCCTGGCAAGGTAACATGGGAGGCAACAAAAAGAAAATCAGAGGGAAAAGATTTAGACCTCGATC TAACTCAACTGAG CTCGAG - GFP Tag - GTTTAA >MG222779 representing NM_001007221
Red=Cloning site Green=Tags(s) MQAAAAASFWLLCVLGTCPLARCGRAGVASLKGLERGKENRFLERQSIIPLRLIYRLGGEDETQHNQLDT RVRGDPGGPQLTHVDKASFRVDAFGTSFVLDVLLNHELLSSGYVERQIEHGGKVVENKGGEHCYYQGQIR GNPVSFVALSTCHGLHGMFYDGNHTYLIEPEENEKSQESSHCHSVYKSRQFEFPLDDLPSEFQRVNITPP QFILKPRLKRRKRQLLRFPRNVEEETKYIELMIVNDHLMFKKHRLSVVYTNTYAKSVVNMADVIYKDQLK TRIVLVAMETWAADNKFAISENPLITLREFMKYRRDFIKEKADAVHLFSGSQFESSRSGAAYIGGICSLL RGGGVNEFGKTDLMAVTLAQSLAHNVGIISDKRKLASGECKCEDTWSGCIMGDTGYYLPKKFTQCNVEEY HDFLNSGGGACLFNKPSKLLDPPECGNGFIETGEECDCGTPAECALEGAECCKKCTLTQDSQCSDGLCCK KCKFQPLGTVCREAVNDCDIREICSGNSSQCAPNVHKMDGYSCDGTQGICFGGRCKTRDRQCKYIWGQKV TASDRYCYEKLNIEGTEKGNCGKDKDTWTQCNKRDVLCGYLLCTNIGNIPRLGELDGEITSTLVVQQGRT LNCSGAHVKLEEDVDLGYVEDGTPCGPQMMCLEHRCLPVASFNFSTCSSSKAGTVCSGNGVCSNELKCVC NRHWTGADCGTHFPHNDDAKTGITLSGNGVAGTNIIIGIIAGTILVLALILGITAWGYKNYREQRSNGLS HSWSERIPDTKHISDICENGRPRSNSWQGNMGGNKKKIRGKRFRPRSNSTE LE - GFP Tag - V |
| Restriction Sites |
SgfI-XhoI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001007221 |
| ORF Size | 2463 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001007221.3, NP_001007222.1 |
| RefSeq Size | 2629 bp |
| RefSeq ORF | 2466 bp |
| Locus ID | 11496 |
| UniProt ID | Q9R1V6 |
| Gene Summary | This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. The protein encoded by this gene is believed to lack metalloproteinase activity due to the lack of a critical catalytic motif. Mice lacking the encoded protein exhibit severe ataxia, hypomyelination and premature death. Alternative splicing results in multiple transcript variants encoding different isoforms, some of which may undergo similar processing. [provided by RefSeq, May 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221842 | Adam22 (untagged) - Mouse a disintegrin and metallopeptidase domain 22 (Adam22), transcript variant 2, (10ug) |
CNY 10,550.00 |
|
| MR222779 | Adam22 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 22 (Adam22), transcript variant 2 |
CNY 8,170.00 |
|
| MR222779L3 | Lenti ORF clone of Adam22 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 22 (Adam22), transcript variant 2 |
CNY 10,070.00 |
|
| MR222779L4 | Lenti ORF clone of Adam22 (mGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 22 (Adam22), transcript variant 2 |
CNY 10,070.00 |
