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Dot1l (NM_199322) Mouse Tagged ORF Clone
CAT＃: MG223041
- TrueORF®
Dot1l (tGFP-tagged) - Mouse DOT1-like histone H3 methyltransferase (S. cerevisiae) (Dot1l), (10ug)
ORF Plasmid: DDK
"NM_199322" in other vectors (4)
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CNY 16,910.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A630076O07; AW907654; Dot1; KMT4; mDot1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223041 representing NM_199322
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGAGAAGCTGGAGCTGAGGCTCAAGTCGCCTGTGGGCGCCGAGCCCGCCGTCTACCCGTGGCCGC TGCCGGTCTACGACAAGCACCATGATGCTGCTCATGAGATTATTGAGACTATCCGGTGGGTCTGCGAGGA AATCCCAGATCTCAAGCTGGCCATGGAGAACTATGTCCTGATCGACTACGACACCAAAAGTTTTGAAAGC ATGCAGCGGCTGTGTGACAAATACAACCGGGCCATCGACAGCATCCACCAGCTGTGGAAGGGCACTACAC AGCCCATGAAGCTGAATACTCGGCCGTCCAATGGACTTCTGCGGCACATCCTGCAGCAGGTATATAACCA CTCTGTCACTGACCCGGAGAAGCTTAACAACTACGAGCCCTTCTCCCCTGAGGTGTATGGGGAGACCTCC TTTGACCTGGTGGCCCAGATGATTGATGAGATCAAGATGACAGAGGATGACCTGTTTGTCGACCTGGGCA GTGGTGTGGGGCAGGTTGTCCTTCAGGTTGCTGCGGCCACCAACTGCAAACATCACTACGGAGTGGAGAA AGCGGACATCCCAGCCAAGTACGCAGAGACCATGGACCGAGAGTTCAGGAAGTGGATGAAATGGTATGGA AAAAAGCATGCAGAATACACACTGGAACGAGGTGACTTCCTCTCAGAGGAATGGAGGGAACGGATCGCCA ACACGAGTGTTATATTTGTGAATAACTTTGCCTTTGGTCCTGAGGTGGATCACCAGCTGAAGGAGCGATT CGCAAACATGAAGGAAGGCGGCAGAATCGTATCCTCAAAGCCCTTTGCCCCTCTGAACTTCAGGATCAAC AGCAGGAACTTGAGTGACATTGGCACCATCATGCGTGTGGTGGAGCTGTCGCCCCTGAAGGGCTCTGTGT CGTGGACTGGCAAGCCTGTCTCCTACTATCTGCACACCATTGACCGCACCATACTTGAAAACTATTTTTC TAGTCTGAAAAATCCAAAACTCAGGGAGGAGCAGGAGGCAGCTAGGCGAAGACAGCAGCGAGAGAACAAG AGCAATGCGACCACGCCCACCAAAGTCCCTGAGAGCAAAGCAGCTGCCACTGAGGCCCCCGCGGATTCTG GTGCTGAGGAAGAAAAATCAGGTGTGGCTACTGTCAAAAAGCCATCTCCCTCCAAAGCCCGGAAGAAGAA ACTGAACAAGAAAGGGAGAAAGATGGCTGGCCGGAAACGTGGGCGGCCCAAGAAAATGAGTGCTGCCAGT GCCGAACGCAAGTCTAAGAAGAGCCAAAGCACACTGGATCTCCTGCACTCTCCGCCCCCAGCCCCACCCT CAGCCTCACCCCAGGATGCGTACAGGGCACCTCACAGCCCATTCTACCAGCTACCTCCAAGCACGCAGCT GCACTCACCCAACCCACTGCTAGTGGCACCCACCCCACCTGCGCTGCAGAAGCTTTTAGAGTCCTTCAGA ATCCAGTACCTGCAGTTCCTGGCTTATACAAAGACCCCGCAGTACAAGGCTAACCTACAGCAGCTTCTGG ACCAGGAGAAGGAGAAGAACACACAGCTCCTGGGCACAGCCCAGCAGCTCTTCGGCCATTGCCAGGCCCA AAAGGAAGAAATCCGCAGGCTGTTCCAGCAGAAGCTGGATGAGTTGGGCGTGAAGGCGCTGACCTACAAT GACCTGATTCAGGCCCAGAAGGAAATCTCTGCCCACAACCAGCAGCTGCGGGAACAGTCGGAACAGCTGG AGAAGGACAACAGCGAGCTTCGGAGCCAGAGCCTGCGGCTGCTCAGGGCCCGGTGTGAGGAGCTGAGGCT TGACTGGTCCACACTGTCCTTGGAGAACCTGCGGAAAGAGAAGCAGGCCCTGAGGAGCCAGATCTCAGAG AAGCAGCGGCACTGCCTGGAGCTGCAGATCAGCATCGTGGAGCTCGAGAAGACCCAGCGCCAGCAGGAGC TCCTGCAGTTAAAGTCCTGTGTACCACCAGATGATGCTTTGTCTTTGCATCTGCGTGGCAAGGGTGCCCT GGGCCGAGAGCTGGAGGCTGATGCTGGGCGCTTACGCCTTGAGCTGGACTGTGCCAAGATCTCCCTGCCA CACCTCAGCAGCATGAGCCCTGAGCTGTCCATGAACGGCCATGCTGCCAGCTATGAGCTCTGCAATGCAG CTAGTCGGCCATCATCCAAGCAGAACACACCCCAGTACCTGGCCTCTCCCTTGGACCAGGAGGTCGTACC TTGTACCCCTAGCCACAGTGGCCGGCCTCGGCTAGAAAAGCTGTCTGGCCTGGCTTTGCCTGATTACACC CGGTTGTCACCTGCCAAGATTGTGTTGAGGCGGCACCTGAGCCAGGACCACACTGGGGCTAGCAAAGCAG CTACCAGTGAGCCACACCCTCGGCCAGAGCACCCCAAGGAGAGCAGCCTTCCCTACCAGAGCCCTGGCTT GTCCAACAGCATGAAGCTCAGCCCCCAGGATCCACCACTGGCCTCCCCGGCAACCTCGCCCCTCACCTCC GAGAAAGGTAGTGAGAAGGGTGTGAAGGAGCGTGCCTACAGCAGCCACGGGGAGACCATCACCAGCCTAC CTGTTAGCATTCCACTCAGCACAGTGCAGCCCAACAAGCTGCCGGTCAGCATCCCACTGGCCAGCGTGGT GCTGCCCAGCCGTGCTGAGAGGGCGAGGAGCACTCCCAGCCCTGTGCCACAGCCCCGGGACTCTTCAGCC ACACTTGAGAAGCAGACTGGTGCTTCTGCCCATGGTGCAGGGGGTGCTGGAGCAGGAAGCAGGAGCCTCG CTGTGGCGCCCACAGGTTTCTATGCTGGTTCGGTGGCCATCAGTGGGGCCCTGGCCAGCAGCCCAGCACC TCTGGCCTCTGGAATGGAATCTGCTGTTTTTGATGAATCCTCTGGCCCCAGCAGCCTCTTTGCCACCATG GGGTCTCGCAGCACACCACCACAACACCCACCTCTCCTGTCGCAGTCCCGCAACTCGGGTCCTGCCTCTC CTGCCCACCAACTCACAGCCAGTCCCCGCCTGAGTGTGACCACTCAGGGCTCGCTGCCAGACACCAGCAA AGGGGAACTGCCTTCTGATCCTGCCTTCTCAGACCCAGAGAGCGAAGCAAAAAGGAGGATTGTGTTCAGC ATTTCAGTTGGTGCCAGCTCCAAGCAGTCACCTTCCACCAGGCATAGCCCCTTGACTTCTGGCACCCGAG GGGACTGTGTGCAGAGCCACGGACAGGACAGTCGTAAGCGCAGCAGAAGGAAGCGTGCATCAGCTGGAAC ACCTAGCCTCAGCACGGGTGTGTCCCCCAAGCGCCGGGCTCTGCCAACTGTTGCTGGCCTCTTTACACAG TCCTCGGGGTCTCCTCTTAATCTCAACTCCATGGTCAGCAACATCAACCAGCCACTGGAGATCACAGCCA TCTCATCCCCTGAGAGCTCCCTGAAGAGCTCCCCCACCCCATACCAGGACCATGACCAGCCTCCGGTGCT CAGGAAGGAACGGCCTCTAGGACTAACTAATGGGGCCCACTACTCGCCACTGACCTCAGATGAGGAGCCA GGCTCTGAGGATGAGCCCAGCAGTGCCCGAATTGAGAGAAAAATTGCAACAATCTCTTTAGAAAGCAAAT CTCCCCCGAAGACACTGGAAAATGGTGGTGGCTTGGTGGGAAGGAAGTCAGCCCCTTCGAGCGAGCCTAT CAACAGCAGCAAATGGAAGTCTACCTTCTCTCCTATCTCTGACCTCGGCTTGGCCAAGGCTGTGGACAGT CCGCTACAGGCTGGCTCTGCCCTGAGCCACAGCCCCCTGTTCTCTTTCCGGCCGTCCCTGGAGGAGCCTG CTGCTGAGGCCAAGCTCCCCACTCACCCAAGGAAAAGCTTTGCGGGCTCTCTGGGTGCAGCCGAGGGGCC AAGCCCTGGCACCAACCCTCCCAACGGCCTGGCCTTCAGTGGGGGCCTTGCTGCAGACCTCGGTTTACAC AGCTTCAATGATGGTGCTTCCCTCTCCCACAAGGGCCCGGATGTGACTGGCCTGAGTGCCTCCCTGAGCT TCCCATCCCAGAGGGGCAAGGACAGTACCACAGAGGCCAACCCTTTCCTCAGCAGGCGGCAGCCCGAGGG CCTGGGTGGCTTGAAGGGTGAGGGCAACGCAAACAAGGAGTCAGGAGAGAGCCTGCCCCTGTGCGGGCCC TCGGACAAGGCCTCACTGCCACATGGCAGCAGGGCCAGCAAAGGTCGTGACCGTGAGCTGGACTTCAAGG GCGGCCACAACCTCTTCATCTCTGCTGCAGCCGTGCCTCCAGGTGGCCTCCTTGGTGGCCCTGGTCTTGT AACTGTGGCTTCTTCTGCGGGCAGTGCAACACCCACTGCCCAGGCTCCCCGGCCTTTCCTGAGCACCTTT GCCCCCGGGCCCCAGTTTACTCTGGGCCCCATGTCCCTGCAGGCCAACCTGGGCTCTGTGGCCGGCTCCT CTGTGCTGCAGTCCTTGTTCAGCACTGTGCCAGCTGCTGCAGGCCTGGTGCATGTGTCGTCCACTGCGAC CCGATTGACCAACTCACACACCATGGGCAGCTTCTCTTCTGGGGTGGCCGGCGGAACCGTTGGAGGTAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223041 representing NM_199322
Red=Cloning site Green=Tags(s) MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFES MQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSNGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETS FDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYG KKHAEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRIN SRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENK SNATTPTKVPESKAAATEAPADSGAEEEKSGVATVKKPSPSKARKKKLNKKGRKMAGRKRGRPKKMSAAS AERKSKKSQSTLDLLHSPPPAPPSASPQDAYRAPHSPFYQLPPSTQLHSPNPLLVAPTPPALQKLLESFR IQYLQFLAYTKTPQYKANLQQLLDQEKEKNTQLLGTAQQLFGHCQAQKEEIRRLFQQKLDELGVKALTYN DLIQAQKEISAHNQQLREQSEQLEKDNSELRSQSLRLLRARCEELRLDWSTLSLENLRKEKQALRSQISE KQRHCLELQISIVELEKTQRQQELLQLKSCVPPDDALSLHLRGKGALGRELEADAGRLRLELDCAKISLP HLSSMSPELSMNGHAASYELCNAASRPSSKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSGLALPDYT RLSPAKIVLRRHLSQDHTGASKAATSEPHPRPEHPKESSLPYQSPGLSNSMKLSPQDPPLASPATSPLTS EKGSEKGVKERAYSSHGETITSLPVSIPLSTVQPNKLPVSIPLASVVLPSRAERARSTPSPVPQPRDSSA TLEKQTGASAHGAGGAGAGSRSLAVAPTGFYAGSVAISGALASSPAPLASGMESAVFDESSGPSSLFATM GSRSTPPQHPPLLSQSRNSGPASPAHQLTASPRLSVTTQGSLPDTSKGELPSDPAFSDPESEAKRRIVFS ISVGASSKQSPSTRHSPLTSGTRGDCVQSHGQDSRKRSRRKRASAGTPSLSTGVSPKRRALPTVAGLFTQ SSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPTPYQDHDQPPVLRKERPLGLTNGAHYSPLTSDEEP GSEDEPSSARIERKIATISLESKSPPKTLENGGGLVGRKSAPSSEPINSSKWKSTFSPISDLGLAKAVDS PLQAGSALSHSPLFSFRPSLEEPAAEAKLPTHPRKSFAGSLGAAEGPSPGTNPPNGLAFSGGLAADLGLH SFNDGASLSHKGPDVTGLSASLSFPSQRGKDSTTEANPFLSRRQPEGLGGLKGEGNANKESGESLPLCGP SDKASLPHGSRASKGRDRELDFKGGHNLFISAAAVPPGGLLGGPGLVTVASSAGSATPTAQAPRPFLSTF APGPQFTLGPMSLQANLGSVAGSSVLQSLFSTVPAAAGLVHVSSTATRLTNSHTMGSFSSGVAGGTVGGN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_199322 |
| ORF Size | 4620 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_199322.2 |
| RefSeq Size | 5694 bp |
| RefSeq ORF | 4623 bp |
| Locus ID | 208266 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224665 | Dot1l (untagged) - Mouse DOT1-like, histone H3 methyltransferase (S. cerevisiae) (Dot1l), (10ug) |
CNY 10,544.00 |
|
| MR223041 | Dot1l (Myc-DDK-tagged) - Mouse DOT1-like, histone H3 methyltransferase (S. cerevisiae) (Dot1l) |
CNY 15,808.00 |
|
| MR223041L3 | Lenti ORF clone of Dot1l (Myc-DDK-tagged) - Mouse DOT1-like, histone H3 methyltransferase (S. cerevisiae) (Dot1l) |
CNY 17,290.00 |
|
| MR223041L4 | Lenti ORF clone of Dot1l (mGFP-tagged) - Mouse DOT1-like, histone H3 methyltransferase (S. cerevisiae) (Dot1l) |
CNY 17,290.00 |
