Pfn4 (NM_028376) Mouse Tagged ORF Clone

CAT#: MG223450

  • TrueORF®

Pfn4 (tGFP-tagged) - Mouse profilin family member 4 (Pfn4), (10ug)

ORF Plasmid: DDK tGFP



  "NM_028376" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 2900024P18Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG223450 representing NM_028376
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTCACTTGCAGAACTTACTGTTAGATACCCTGCTGGGGACGAAACACGTGGACAGCGCAGCCCTCA
TCAAACTCCAGGAAAAGACCCTGTGTGTGACATCACCAGGATTTAGTGTAATGCCAAGTGATGTTCGAAC
ACTTCTGAATGGATTTGCCAAAAACCCATTATTAACGCGAAGGGAAGGGTTGTATTTCAAGGAGAAGGAT
TACAAATGTGTTCGGGCAGATGACTATTCTCTCTATGCTAAGAATGAGAACACTGGTGTGGTGGTGGTGA
AGACCAACATGTATCTTGTGGTGGCAACTTACACTGCAGGCATGTATCCTAGCGTCTGCGTGGAAGCCAC
AGAGAAGCTGGGAGAGTATCTAAGGAAAAAGGGAAAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG223450 representing NM_028376
Red=Cloning site Green=Tags(s)

MSHLQNLLLDTLLGTKHVDSAALIKLQEKTLCVTSPGFSVMPSDVRTLLNGFAKNPLLTRREGLYFKEKD
YKCVRADDYSLYAKNENTGVVVVKTNMYLVVATYTAGMYPSVCVEATEKLGEYLRKKGN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_028376
ORF Size 387 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_028376.4
RefSeq Size 1306 bp
RefSeq ORF 390 bp
Locus ID 382562
UniProt ID Q9D6I3
Gene Summary Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), phosphatidylinositol 4-phosphate (PtdIns(4)P) and phosphatidic acid (PA). Does not bind to actin, contrary to other family members (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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