Map2k2 (NM_023138) Mouse Tagged ORF Clone
CAT#: MG223494
- TrueORF®
Map2k2 (tGFP-tagged) - Mouse mitogen-activated protein kinase kinase 2 (Map2k2), (10ug)
ORF Plasmid: DDK
"NM_023138" in other vectors (4)
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CNY 3,710.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AA589381; MEK2; MK2; Prkmk2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223494 representing NM_023138
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGCCCGGAGGAAGCCGGTGTTACCGGCACTCACTATCAACCCTACCATCGCCGAGGGCCCGTCCC CCACCAGCGAGGGCGCCTCTGAGGCAAACCTGGTGGACCTCCAGAAGAAGCTGGAAGAGCTGGACCTGGA TGAGCAGCAAAGGAAGCGGCTGGAGGCCTTCCTTACCCAGAAGGCTAAGGTCGGTGAGCTCAAGGACGAC GACTTTGAGAGGATCTCAGAGCTGGGTGCCGGCAATGGCGGAGTGGTCACCAAGGCCCGGCATAGGCCGT CAGGCCTCATCATGGCCAGAAAGCTGATCCACCTGGAGATCAAACCGGCCGTGCGCAACCAGATCATCCG GGAGCTGCAGGTGCTGCACGAGTGCAACTCGCCCTACATCGTGGGCTTCTATGGGGCCTTCTACAGCGAC GGCGAGATCAGCATCTGCATGGAGCACATGGATGGTGGCTCACTGGACCAGGTACTGAAGGAGGCCAAGC GGATTCCTGAAGACATCTTGGGGAAGGTCAGCATTGCGGTGCTCCGGGGCCTGGCCTACCTCCGAGAGAA GCACCAGATCATGCACAGAGATGTGAAGCCCTCCAACATCCTGGTGAACTCTCGCGGGGAGATTAAGCTG TGTGACTTCGGGGTGAGCGGCCAGCTGATCGACTCCATGGCCAACTCGTTTGTAGGGACGCGCTCCTACA TGTCCCCAGAGCGGCTGCAGGGCACCCACTACTCTGTGCAGTCGGACATCTGGAGCATGGGGCTGTCGCT GGTGGAGCTGGCCATCGGGAGGTATCCCATTCCCCCACCTGATGCCAAGGAACTAGAGGCCAGCTTTGGC CGGCCTGTGGTGGACGGGGCAGACGGAGAACCCCATAGTGTCTCCCCGAGGCCCAGGCCCCCTGGACGCC CCATCAGTGGTCATGGGATGGACAGCCGACCGGCCATGGCCATCTTTGAGCTGCTGGACTACATAGTGAA CGAGCCACCTCCCAAGCTGCCCAGTGGTGTGTTCAGCTCAGACTTCCAGGAGTTTGTGAATAAATGTCTC ATTAAGAACCCAGCAGAGCGAGCAGATCTGAAGCTGCTGATGAACCACGCCTTCATCAAGCGCTCTGAGG GGGAGGAAGTGGACTTCGCCGGCTGGCTGTGCAGAACCCTGCGGCTGAAGCAGCCCAGCACACCCACGCG GACTGCAGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223494 representing NM_023138
Red=Cloning site Green=Tags(s) MLARRKPVLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEELDLDEQQRKRLEAFLTQKAKVGELKDD DFERISELGAGNGGVVTKARHRPSGLIMARKLIHLEIKPAVRNQIIRELQVLHECNSPYIVGFYGAFYSD GEISICMEHMDGGSLDQVLKEAKRIPEDILGKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKL CDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVELAIGRYPIPPPDAKELEASFG RPVVDGADGEPHSVSPRPRPPGRPISGHGMDSRPAMAIFELLDYIVNEPPPKLPSGVFSSDFQEFVNKCL IKNPAERADLKLLMNHAFIKRSEGEEVDFAGWLCRTLRLKQPSTPTRTAV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_023138 |
ORF Size | 1203 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_023138.4, NP_075627.2 |
RefSeq Size | 2387 bp |
RefSeq ORF | 1206 bp |
Locus ID | 26396 |
UniProt ID | Q63932 |
Gene Summary | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209716 | Map2k2 (untagged) - Mouse mitogen-activated protein kinase kinase 2 (Map2k2), (10ug) |
CNY 3,990.00 |
|
MR223494 | Map2k2 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase 2 (Map2k2) |
CNY 3,656.00 |
|
MR223494L3 | Lenti ORF clone of Map2k2 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase 2 (Map2k2) |
CNY 5,230.00 |
|
MR223494L4 | Lenti ORF clone of Map2k2 (mGFP-tagged) - Mouse mitogen-activated protein kinase kinase 2 (Map2k2) |
CNY 5,230.00 |