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Mink1 (NM_001045959) Mouse Tagged ORF Clone
CAT＃: MG223913
- TrueORF®
Mink1 (tGFP-tagged) - Mouse misshapen-like kinase 1 (zebrafish) (Mink1) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_001045959" in other vectors (2)
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CNY 14,630.00
货期*
7周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | B55; Map4k6; MINK; Ysk2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223913 representing NM_001045959
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACCCAGCCCCCGCCCGCAGCCTGGACGACATCGACCTGTCTGCCCTGCGGGACCCTGCAGGAA TCTTTGAGCTGGTGGAGGTGGTTGGCAATGGAACCTATGGACAGGTATACAAGGGGCGGCACGTCAAGAC TGGGCAGCTGGCTGCCATTAAGGTCATGGATGTCACAGAGGATGAGGAGGAAGAGATCAAACAGGAAATC AACATGTTAAAGAAGTACTCTCACCATCGCAATATTGCCACCTACTATGGGGCCTTTATCAAGAAGAGCC CTCCTGGGAACGATGACCAGCTCTGGCTGGTGATGGAGTTCTGCGGTGCTGGTTCAGTGACCGACCTGGT AAAGAACACAAAAGGGAACGCACTGAAGGAGGATTGCATTGCTTACATCTGCAGGGAGATTCTCAGGGGT CTTGCCCATCTCCATGCCCACAAGGTGATCCACAGAGATATCAAGGGACAAAATGTGCTGCTGACAGAGA ATGCTGAAGTCAAGCTAGTGGATTTTGGGGTGAGTGCTCAGCTGGACCGCACTGTGGGCAGGCGGAACAC TTTCATTGGAACCCCATACTGGATGGCTCCAGAAGTCATTGCCTGTGACGAGAACCCCGATGCCACCTAT GACTACAGGAGTGACATTTGGTCTCTAGGAATCACAGCCATTGAAATGGCAGAGGGAGCCCCCCCTCTGT GTGACATGCACCCTATGCGGGCCCTCTTCCTCATCCCTCGGAACCCTCCCCCCAGGCTCAAGTCAAAGAA ATGGTCTAAGAAGTTCACTGACTTCATCGACACGTGTCTCATCAAGACTTACCTGAGCCGCCCACCCACC GAACAGTTACTCAAATTCCCCTTCATCCGAGACCAGCCCACGGAGCGGCAGGTCCGCATCCAGCTCAAGG ACCACATCGACCGCTCGCGGAAGAAGCGGGGTGAGAAAGAGGAGACAGAGTATGAGTACAGCGGCAGTGA GGAGGAAGACGACAGCCATGGAGAGGAAGGCGAGCCAAGCTCCATCATGAATGTGCCCGGTGAGTCCACA CTGCGCAGAGAATTCCTCAGACTCCAGCAGGAGAATAAGAGCAACTCTGAGGCTTTAAAGCAGCAGCAGC AGCTGCAGCAACAGCAGCAGCGGGACCCGGAGGCACACATCAAACACCTGCTGCACCAGCGGCAGCGTCG CATAGAGGAGCAGAAGGAGGAGCGGCGACGTGTGGAGGAGCAACAGCGGCGAGAGCGAGAACAGCGTAAG CTACAAGAGAAGGAGCAGCAGCGGCGATTGGAAGACATGCAAGCCCTACGACGAGAGGAAGAGAGGCGGC AAGCAGAGCGGGAACAGGAATACAAGCGGAAGCAGCTGGAGGAGCAGCGGCAGTCAGAGCGGCTGCAGAG ACAGCTGCAGCAGGAGCACGCCTACCTCAAGTCCCTGCAGCAGCAGCAGCAGCAGCAGCAGCTCCAGAAG CAGCAGCAGCAGCAGCAGCAGATCCTGCCTGGAGACAGGAAGCCCCTGTATCATTACGGTCGGGGCATTA ATCCTGCTGACAAGCCAGCATGGGCCCGCGAGGTGGAAGAGAGAGCACGGATGAACAAGCAGCAGAACTC TCCCTTGGCGAAGGCGAAGCCAAGCAGTGCGGGGCCAGAGCCCCCCATCTCCCAGGCCTCTCCTAGCCCC CCAGGACCTCTTTCCCAGACTCCTCCTATGCAGAGGCCTGTGGAGCCCCAGGAAGGACCGCACAAGAGCC TGGTGGCACACCGGGTCCCACTGAAGCCATATGCAGCACCTGTACCCCGATCCCAGTCCCTGCAGGACCA GCCGACTCGAAACCTGGCTGCCTTCCCAGCCTCCCACGACCCTGACCCTGCTGCTGTCCCTACACCCACT GCCACACCCAGTGCCCGAGGAGCTGTCATCCGCCAGAATTCAGACCCCACCTCTGAAGGGCCAGGGCCTA GCCCAAACCCTCCATCCTGGGTTCGGCCTGATAATGAGGCTCCACCTAAGGTTCCACAGAGGACCTCTTC TATCGCCACTGCCCTTAACACCAGTGGGGCCGGAGGGTCCCGGCCAGCTCAGGCTGTCCGTGCCAGACCT CGCAGTAACTCCGCCTGGCAAATCTATCTGCAGAGGCGGGCAGAGCGGGGCACCCCCAAGCCTCCCGGGC CCCCAGCTCAGCCCCCTGGCCCGCCCAACGCCTCTAGTAACCCTGACCTCAGGAGGAGTGACCCTGGCTG GGAGCGCTCAGACAGTGTCCTCCCGGCCTCCCACGGCCACCTCCCTCAGGCTGGCTCCTTGGAGCGGAAC CGAAACCGTGTGGGAGCCTCCACAAAACTGGATAGCTCTCCAGTGCTCTCCCCTGGGAACAAAGCCAAGC CTGAAGACCACCGCTCAAGGCCAGGCCGGCCCGCAGACTTTGTGTTGCTCAAAGAGCGGACTCTGGATGA GGCCCCTAAGCCTCCCAAGAAGGCCATGGACTACTCCTCATCCAGTGAGGAGGTGGAAAGCAGTGAAGAG GAGGAGGAGGAAGGCGATGGGGAGCCGTCAGAGGGGAGCAGAGACACTCCCGGGGGCCGCAGTGATGGTG ATACAGACAGCGTCAGCACCATGGTGGTTCATGATGTTGAGGAGATATCCGGGACCCAGCCCTCATATGG CGGCGGCACCATGGTGGTCCAGCGTACTCCTGAAGAGGAACGAAGCCTGCTGCTTGCTGATAGCAATGGC TACACAAACCTGCCTGATGTGGTCCAGCCCAGCCACTCACCTACTGAGAACAGCAAAGGTCAAAGCCCTC CAACAAAGGATGGAGGCAGTGATTACCAGTCTCGTGGGCTGGTAAAGGCCCCAGGAAAGAGCTCATTCAC CATGTTTGTGGATCTAGGGATCTACCAGCCTGGAGGCAGTGGGGACACCATCCCTATCACAGCCCTAGTG GGTGGAGAAGGTGGTCGCCTTGATCAACTGCAGTTCGATGTGAGGAAGGGCTCTGTGGTCAACGTCAATC CCACCAACACCCGAGCTCATAGTGAAACTCCTGAAATTCGCAAGTACAAGAAGCGATTCAACTCAGAGAT CCTATGTGCAGCTCTCTGGGGGGTCAACCTCCTAGTGGGCACAGAGAATGGGCTGATGTTGCTGGACCGA AGTGGGCAGGGCAAGGTGTATGGACTTATTGGGCGACGACGCTTCCAGCAAATGGATGTCTTAGAAGGGC TCAACTTGCTCATCACCATCTCAGGGAAAAGGAACAAACTGCGGGTATATTACCTGTCCTGGCTTCGGAA CAAGATCCTACACAATGACCCAGAGGTGGAAAAGAAGCAGGGGTGGACCACCGTGGGGGACATGGAGGGC TGCGGCCACTACCGTGTTGTGAAATATGAACGGATTAAGTTCCTGGTCATTGCCCTGAAGAACTCCGTGG AGGTTTATGCCTGGGCTCCCAAACCCTACCACAAATTCATGGCCTTCAAGTCCTTTGCTGACCTCCCTCA CCGCCCTCTACTGGTGGACCTGACAGTAGAGGAGGGACAGCGGCTCAAGGTCATCTATGGCTCCAGTGCT GGCTTCCATGCTGTGGATGTTGATTCTGGGAACAGCTATGACATCTACATCCCTGTACATATCCAGAGCC AGATCACACCCCACGCCATCATCTTCCTCCCCAACACTGATGGCATGGAGATGCTGCTGTGCTATGAAGA TGAGGGTGTCTATGTCAACACTTACGGGCGGATCATCAAGGATGTGGTGCTGCAGTGGGGAGAGATGCCC ACCTCTGTGGCCTACATCTGCTCCAACCAGATAATGGGCTGGGGTGAGAAGGCCATAGAGATCCGCTCTG TGGAGACAGGCCACCTAGATGGGGTCTTCATGCACAAACGAGCCCAGAGGCTCAAGTTCCTGTGTGAGCG CAATGACAAGGTGTTTTTTGCCTCTGTCCGCTCTGGAGGAAGCAGCCAAGTTTACTTTATGACTCTGAAC CGTAACTGCATCATGAACTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223913 representing NM_001045959
Red=Cloning site Green=Tags(s) MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEI NMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRG LAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATY DYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPT EQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGEST LRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRK LQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQK QQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSP PGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPT ATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARP RSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERN RNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPADFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEE EEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNG YTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALV GGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDR SGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEG CGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSA GFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMP TSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLN RNCIMNW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001045959 |
| ORF Size | 4011 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001045959.1, NP_001039424.1 |
| RefSeq Size | 4973 bp |
| RefSeq ORF | 4014 bp |
| Locus ID | 50932 |
| Gene Summary | Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1.[UniProtKB/Swiss-Prot Function] |
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