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Alox12b (NM_009659) Mouse Tagged ORF Clone
CAT＃: MG223970
- TrueORF®
Alox12b (tGFP-tagged) - Mouse arachidonate 12-lipoxygenase 12R type (Alox12b), (10ug)
ORF Plasmid: DDK
"NM_009659" in other vectors (4)
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CNY 6,840.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 12R-; 12R-LOX; Alo; Aloxe2; e-LO; e-LOX2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223970 representing NM_009659
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCTATAAAGTCAAGGTGGCCACGGGCACTGACTTCTTTTCGGGGACGCTGGATTCAATTTCAC TGACCATCGTGGGGACCCAAGGAGAGAGCCATAAGCAGCGGTTGAACCACTTTGGGAGAGACTTTGCCAC GGGGGCGGTGGATGACTACACGGTACAGTGCCAGCAGGACCTGGGGGAGCTTATCATCATCCGTCTGCAC AAGGAGCCTCATTCCTTCCTAGCCAAGGACCCCTGGTACTGCAACTATGTGCAGATCTGTGCTCCCGACT GCCGTGTCTACCACTTCCCAGCTTACCAGTGGATGGATGGCTATGAGACCCTGGCACTCCGGGAGGCCAC AGGAAAGATAACAGCAGACGACACACTCCCTATCCTTTTGGAACACAGACAAGAAGAGATCAGAGCCAAG AAGGACTTCTACCACTGGAGGGTCTTTGTTCCCGGCCTTCCCAACTATGTGGATATCCCCAGTTACCATC CTCCTCCCCGACGGTGCCGCAATCCCAACCGGCCTGAGTGGGATGGTTATATTCCCGGATTCCCAATTCT CATCAACATTAAGGCTACCAGGTTCTTAAACTCAAATCTTCGTTTCTCCTTCGTCAAGACAGCCTCCTTC TTCTACCGCCTAGGGCCCATGGCGCTGGCCTTCAAACTGCGTGGCCTGGTGGATCGAAAACGCTCCTGGA AGAGACTAAAGGACATTAAGAATATTTTCCCTGCTACCAAGTCTGTCGTCTCAGAGTATGTGGCCGAGCA CTGGACAGAGGACAGCTTCTTTGGGTACCAGTACCTTAATGGTATTAACCCAGGCCTGATCCGAAGATGT ACACAGATCCCAGACAAATTCCCAGTCACAGATGAAATGGTGGCCCCATTCCTGGGTGAAGGAACATGCC TGCAAGCAGAGCTGGAGAGGGGGAATATTTACCTGGCAGACTACCGCATCCTGGATGGCATCCCCACCGT GGAGCTCAATGGTCAGCAGCAGCATCACTGTGCCCCGATGTGCTTGCTGCACTTTGGTCCTGATGGCAAC ATGATGCCCATCGCCATTCAGCTCAGTCAGACTCCTGGGCCTGATTGTCCTATCTTCCTGCCGAATGATT CTGAGTGGGACTGGCTGTTGGCTAAAACGTGGGTGCGCTACGCGGAGTTCTACAGCCACGAGGCCGTGGC ACACTTGCTGGAGAGCCACCTCATCGGGGAAGCTTTCTGCTTGGCCCTCCTGAGGAACCTGCCCATGTGC CACCCCCTGTATAAGCTTCTCATCCCTCACACTCGGTACAACGTCCAGATCAATAGCATTGGGCGGGCCC TCCTTCTCAACAAGGGAGGTCTCTCTGCCAGGGCCATGTCCCTGGGCCTAGAGGGCTTCGCACAGGTGAT GGTCCGGGGTCTGTCTGAGCTCACCTACAAAAGCCTCTGCATTCCCAACGACTTTGTGGAGCGCGGGGTC CAGGACCTGCCTGGGTATTATTTCCGTGATGACAGCCTGGCGGTGTGGTACGCGATGGAGAGGTACGTGA CAGAGATCATCACTTATTATTACCCAAATGACGCAGCCGTAGAGGGCGACCCGGAACTGCAGTGCTGGGT ACAGGAGATATTCAAGGAGTGTCTCCTGGGGCGTGAGAGCTCAGGCTTCCCCACCTGCTTGCGAACTATA CCTGAGCTGATCGAATACGTCACAATGGTCATGTACACCTGCTCGGCCAGGCATGCAGCTGTCAACTCAG GCCAGCTGGAGTACACCTCTTGGATGCCCAACTTCCCATCATCCATGAGGAACCCACCGATGCAGACCAA GGGGCTAACTACTCTGCAGACATACATGGACACTCTTCCGGACGTGAAGACCACATGCATTGTGCTCTTG GTGCTCTGGACTCTCTGTCGGGAGCCTGATGACAGGCGGCCGCTTGGCCACTTCCCAGACATCCATTTTG TGGAGGAGGGTCCTCGGAGGAGTATTGAGGCTTTTCGTCAGAACCTCAACCAGATCTCTCACAACATCCG ACAGCGCAATAAGTGCCTCACCCTCCCGTACTACTACCTGGACCCAGTGCTGATTGAGAACAGTATTTCC ATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223970 representing NM_009659
Red=Cloning site Green=Tags(s) MATYKVKVATGTDFFSGTLDSISLTIVGTQGESHKQRLNHFGRDFATGAVDDYTVQCQQDLGELIIIRLH KEPHSFLAKDPWYCNYVQICAPDCRVYHFPAYQWMDGYETLALREATGKITADDTLPILLEHRQEEIRAK KDFYHWRVFVPGLPNYVDIPSYHPPPRRCRNPNRPEWDGYIPGFPILINIKATRFLNSNLRFSFVKTASF FYRLGPMALAFKLRGLVDRKRSWKRLKDIKNIFPATKSVVSEYVAEHWTEDSFFGYQYLNGINPGLIRRC TQIPDKFPVTDEMVAPFLGEGTCLQAELERGNIYLADYRILDGIPTVELNGQQQHHCAPMCLLHFGPDGN MMPIAIQLSQTPGPDCPIFLPNDSEWDWLLAKTWVRYAEFYSHEAVAHLLESHLIGEAFCLALLRNLPMC HPLYKLLIPHTRYNVQINSIGRALLLNKGGLSARAMSLGLEGFAQVMVRGLSELTYKSLCIPNDFVERGV QDLPGYYFRDDSLAVWYAMERYVTEIITYYYPNDAAVEGDPELQCWVQEIFKECLLGRESSGFPTCLRTI PELIEYVTMVMYTCSARHAAVNSGQLEYTSWMPNFPSSMRNPPMQTKGLTTLQTYMDTLPDVKTTCIVLL VLWTLCREPDDRRPLGHFPDIHFVEEGPRRSIEAFRQNLNQISHNIRQRNKCLTLPYYYLDPVLIENSIS I TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009659 |
| ORF Size | 2103 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009659.2, NP_033789.1 |
| RefSeq Size | 2347 bp |
| RefSeq ORF | 2106 bp |
| Locus ID | 11686 |
| UniProt ID | O70582 |
| Gene Summary | This gene encodes an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene can prevent the formation of the epidermal permeability barrier and cause an ichthyosiform phenotype. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220562 | Alox12b (untagged) - Mouse arachidonate 12-lipoxygenase, 12R type (Alox12b), (10ug) |
CNY 9,030.00 |
|
| MR223970 | Alox12b (Myc-DDK-tagged) - Mouse arachidonate 12-lipoxygenase, 12R type (Alox12b) |
CNY 5,144.00 |
|
| MR223970L3 | Lenti ORF clone of Alox12b (Myc-DDK-tagged) - Mouse arachidonate 12-lipoxygenase, 12R type (Alox12b) |
CNY 8,080.00 |
|
| MR223970L4 | Lenti ORF clone of Alox12b (mGFP-tagged) - Mouse arachidonate 12-lipoxygenase, 12R type (Alox12b) |
CNY 8,080.00 |
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