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Sacm1l (NM_030692) Mouse Tagged ORF Clone
CAT＃: MG224002
- TrueORF®
Sacm1l (tGFP-tagged) - Mouse SAC1 (suppressor of actin mutations 1 homolog)-like (S. cerevisiae) (Sacm1l), (10ug)
ORF Plasmid: DDK
"NM_030692" in other vectors (4)
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CNY 6,460.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | mKIAA0851; SA; SAC1; Sac1p |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224002 representing NM_030692
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGCAGCCTACGAGCATCTGAAGCTGCATATTACACCTGAAAAGTTTTATGTGGAGGCTTGTG ATGATGGAGCAGATGATGTACTCATCATTGACCGAGTGTCCACAGAGGTCACCCTTGCAGTCAAAAAAGA TGTTCCCCCCTCTGCTGTTACAAGACCAATATTTGGGATACTGGGCACAATCCATCTGGTGGCAGGTAAT TATCTTGTTGTCATCACCAAAAAGATGAAAGTAGGTGAATGTTTTAATCATGCGGTCTGGAGAGCCACAG ATTTTGATGTTCTTTCTTATAAGAAGACAATGTTGCACTTGACTGATATTCAGTTACAAGATAATAAAAC TTTCCTAGCAATGCTAAACCATGTCTTGAGTATGGATGGATTTTACTTTTCAACAACATATGACTTGACT CATACTTTGCAGCGACTATCTAACACGAGTCCAGAATTCCAAGAAATGAGTCTCTTAGAAAGGGCAGATC AGCGGTTTGTATGGAACGGGCATCTGCTCAGGGAACTGTCTGCTCAGCCAGAGGTCCATCGGTTTGCTCT TCCAGTGTTGCATGGCTTTATTACTATGCACTCATGTTCTATTAATGGAAAATATTTTGATTGGATTCTC ATCTCCCGAAGGAGTTGTTTCAGAGCTGGTGTTCGTTATTATGTGAGAGGAATTGATTCTGAAGGCCACG CAGCTAACTTTGTAGAAACAGAGCAGATTGTGCACTACAGCGGGAACAGGGCTTCGTTTGTCCAGACTCG AGGATCAATACCTATTTTCTGGTCACAGAGACCAAATCTAAAGTACAAACCACATCCACAGATCAGCAAA GTAGCAAATCACATGGATGGTTTCCAGAGGCATTTTGATTCACAAGTAATTATTTATGGAAAGCAAGTAA TAATTAATCTGGTGAACCACAAGGGATCAGAGAAACCACTTGAGCAAACATTCGCAAACATGGTGTCCTC CTTGGGAAGTGGGATGATCAGATATATTGCCTTTGACTTCCATAAGGAGTGTAAAAATATGAGATGGGAT CGATTGAGTATTTTATTGGATCAAGTAGCAGAAATGCAGGATGAATTAAGTTATTTTTTAGTAGACTCTG CCGGCAAGGTGGTGACAAACCAGGACGGAGTTTTCCGCAGCAACTGCATGGATTGTCTAGACAGAACGAA TGTGATACAGAGTTTATTAGCTCGACGTTCCCTTCAGGCACAGCTGCAGAGGCTAGGAGTTTTGCATGTG GGACAGAAGCTTGAAGAGCAAGATGAATTTGAGAAGACTTACAAAAATGCCTGGGCCGATAATGCTAATG CTTGTGCCAAACAGTATGCGGGAACCGGTGCCTTGAAGACTGACTTTACAAGAACTGGAAAAAGAACTCA GTTGGGACTATTAATGGATGGCTTCAACTCATTATTACGGTATTACAAGAACAACTTTTCTGATGGATTT AGACAAGATTCCATAGACTTATTTCTTGGAAATTATTCAGTGGATGAATTAGAATCTCATAGCCCTTTAA GTGTTCCAAGGGACTGGAAATTCCTGGCGTTGCCTATCATCATGGTTGTTGCCTTTTCAATGTGCATCAT CTGTTTGCTTATGGCTGGTGACACTTGGACAGAAACACTGGCATATGTCCTCTTCTGGGGAGTTGCAAGC ATTGGAACATTTTTTATTATTCTTTACAATGGCAAAGATTTTGTTGATGCTCCCAGACTGGTCCAGAAAG AAAAGATAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224002 representing NM_030692
Red=Cloning site Green=Tags(s) MAAAAYEHLKLHITPEKFYVEACDDGADDVLIIDRVSTEVTLAVKKDVPPSAVTRPIFGILGTIHLVAGN YLVVITKKMKVGECFNHAVWRATDFDVLSYKKTMLHLTDIQLQDNKTFLAMLNHVLSMDGFYFSTTYDLT HTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWIL ISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYSGNRASFVQTRGSIPIFWSQRPNLKYKPHPQISK VANHMDGFQRHFDSQVIIYGKQVIINLVNHKGSEKPLEQTFANMVSSLGSGMIRYIAFDFHKECKNMRWD RLSILLDQVAEMQDELSYFLVDSAGKVVTNQDGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHV GQKLEEQDEFEKTYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQLGLLMDGFNSLLRYYKNNFSDGF RQDSIDLFLGNYSVDELESHSPLSVPRDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVAS IGTFFIILYNGKDFVDAPRLVQKEKID TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030692 |
| ORF Size | 1761 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030692.5 |
| RefSeq Size | 3433 bp |
| RefSeq ORF | 1764 bp |
| Locus ID | 83493 |
| UniProt ID | Q9EP69 |
| Gene Summary | This gene encodes an integral membrane protein, which is localized to the endoplasmic reticulum, and functions as a phosphoinositide phosphatase that hydrolyzes phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, and phosphatidylinositol 3,5-bisphosphate. Deletion of this gene in mouse results in preimplantation lethality. Other studies suggest that this gene is also involved in the organization of golgi membranes and mitotic spindles. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Dec 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219440 | Sacm1l (untagged) - Mouse SAC1 (suppressor of actin mutations 1, homolog)-like (S. cerevisiae) (Sacm1l), (10ug) |
CNY 7,510.00 |
|
| MR224002 | Sacm1l (Myc-DDK-tagged) - Mouse SAC1 (suppressor of actin mutations 1, homolog)-like (S. cerevisiae) (Sacm1l) |
CNY 6,552.00 |
|
| MR224002L3 | Lenti ORF clone of Sacm1l (Myc-DDK-tagged) - Mouse SAC1 (suppressor of actin mutations 1, homolog)-like (S. cerevisiae) (Sacm1l) |
CNY 7,790.00 |
|
| MR224002L4 | Lenti ORF clone of Sacm1l (mGFP-tagged) - Mouse SAC1 (suppressor of actin mutations 1, homolog)-like (S. cerevisiae) (Sacm1l) |
CNY 7,790.00 |
