Trmt1 (NM_001164560) Mouse Tagged ORF Clone
CAT#: MG224696
- TrueORF®
Trmt1 (tGFP-tagged) - Mouse TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) (Trmt1) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_001164560" in other vectors (3)
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CNY 7,220.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 6720406L13Rik; 6720477L24; D8Ertd812e |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224696 representing NM_001164560
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGAGAAGCATGCACCGTGACTCGTTGGATCCTCCTCTGTAGAGCTCAGTTCATGGAACGGAAGGCCC AGAAGCCTCCCTCTCCACCAGCGATGGAGAACGGCACCCGGTCCTGGGAAGAGCGCCCACCCGCGGACCC GGTTGCGACTGTCACTGAGGGCGCCGCCAAAATCGTCTTTCCCAGTGCTAACGAGGTTTTCTATAACCCG GTGCAGGAATTCAACCGGGACCTGACGTGTGCTGTGATCACAGAGTTTGCTCGAATTCATCTTGGGGCCA AGGGAATCCAGATCAAGGTTCCAGGAGAGAAGGACTCAGAGAAAATAGCTGTGGACTTGTCTGATCAAGA AGAGGAAACAGCTGGAAAGAATGAAAATCTGGCCCCAGGGGACTGGCCACGGACTGCTGCAGTGGGTGAG ATCTGTGAGGAAGGTCTACGAGTGCTGGAAGGCCTGGCAGCTTCCGGTCTGCGCTCCATCCGTTTTGCCC TGGAGGTTCCTGGCCTCCAGTCTGTGGTTGCCAATGATGCCTCTGCCCGGGCTGTAGAGCTTATGCACAG AAATGTGGAGCTCAATGGTGTGGCACATCTTGTTCAGCCCAACCAGGCAGATGCCCGGATGCTGATGTAC CAACACCAAAAGGCACCTGAGCGATTTGATGTCATTGACCTGGACCCCTATGGCAGCCCCGCCCCCTTCC TGGATGCAGCAGTGCAGGCTGTGAGTGATGGAGGACTTCTGTGTGTCACCTGCACAGACATGGCAGTCCT GGCGGGGAACAGCGGAGAGACATGCTACAGCAAGTATGGGGCCATGGCCCTCAAGAGCCGAGCCTGCCAT GAAATGGCCCTGCGGATTGTGCTGCACAGCCTGGACCTGCACGCCAACTGCTACCAGCGCTATATCGTGC CGCTGCTCAGCATCAGTGCTGACTTCTATATCCGGGTCTTTGTACGTGTTTTCACAGGCCAGGCCAAGGT TAAGTCCTCAGCCAGCAAACAGGCCCTGGTGTTCCAGTGTGTGGGTTGTGGAGCCTTCTACCTGCAACGC CTTGGCAAAGCATCAGGAGACCCTGGTGGCAGGATCAAGTTCTCTGCAGCCTGTGGTCCTCCAGTAACTC CCGAGTGTGAGCACTGTGGACAGAGACACCAGCTTGGGGGCCCCATGTGGGCAGAGCCCATCCATGACCT GGACTTTGTGGGCCGAGTTCTTGACGCAGTGACCACGAATCCTGGCCGCTTCCACACTTCCATGAGGATC CAAGGCGTCCTCAGTGTTGTTACTGAGGAACTTCCTGATGTCCCTCTCTACTACACACTGGACCAACTGA GCAGCACTATCCACTGCAACACACCCCGCCTTCTGCAGCTTCGGTCAGCTCTCCTTCATGCTGGTTTCCG AGTCTCTCTCTCCCATGCTTGTAAGAATGCAGTGAAGACAGATGCTCCCCCTGAGGCCCTCTGGGACATC ATGCGTTGCTGGGAAAAGGAATGTCCAGTGAAACGGGAGCGGCTGTCTGAAAGCAGTCCAGCATTCCGAA TTCTTGCTGTGGAGCCCAGGCTGAAGGCCAACTTCAACATCCGGGAAGACGCCAACCCCAGCTCCCGCCA GCGAGGACTTAAGCGCTTCCAGGCCAATCCAGAAGCCAACTGGGGTCCCCGGCCCCGTGCCCGGCCAGGG GGCAAGGCAGCAAGTGAAGATCTAGCAGGGAGGCGGAGGCTGCTACAGAATAAGAGGAAGGAGCCAGCTG AGGACCCGGCCCAGCGTGCAGCTCGCCTTAAGACATTTCCTTGCAAACGATTCAAAGAGGGTACCTGTCA ACTAGGGGACCAGTGCTGTTATTCACACAGTCCTGCAGCTCCTGTGGCCTCTGGTGATATCCCCATCGAA GAATGTCCAGAGACTACCACCAAGATCTCCCCTGGACCAAAGGCTGCTGCTGGGGGTATTCCTGGGCCAG GTGTAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224696 representing NM_001164560
Red=Cloning site Green=Tags(s) MREACTVTRWILLCRAQFMERKAQKPPSPPAMENGTRSWEERPPADPVATVTEGAAKIVFPSANEVFYNP VQEFNRDLTCAVITEFARIHLGAKGIQIKVPGEKDSEKIAVDLSDQEEETAGKNENLAPGDWPRTAAVGE ICEEGLRVLEGLAASGLRSIRFALEVPGLQSVVANDASARAVELMHRNVELNGVAHLVQPNQADARMLMY QHQKAPERFDVIDLDPYGSPAPFLDAAVQAVSDGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACH EMALRIVLHSLDLHANCYQRYIVPLLSISADFYIRVFVRVFTGQAKVKSSASKQALVFQCVGCGAFYLQR LGKASGDPGGRIKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLDAVTTNPGRFHTSMRI QGVLSVVTEELPDVPLYYTLDQLSSTIHCNTPRLLQLRSALLHAGFRVSLSHACKNAVKTDAPPEALWDI MRCWEKECPVKRERLSESSPAFRILAVEPRLKANFNIREDANPSSRQRGLKRFQANPEANWGPRPRARPG GKAASEDLAGRRRLLQNKRKEPAEDPAQRAARLKTFPCKRFKEGTCQLGDQCCYSHSPAAPVASGDIPIE ECPETTTKISPGPKAAAGGIPGPGVD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001164560 |
ORF Size | 1968 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001164560.1, NP_001158032.1 |
RefSeq Size | 2083 bp |
RefSeq ORF | 1971 bp |
Locus ID | 212528 |
UniProt ID | Q3TX08 |
Gene Summary | Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR224696 | Trmt1 (Myc-DDK-tagged) - Mouse TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) (Trmt1), transcript variant 3 |
CNY 6,560.00 |
|
MR224696L3 | Lenti ORF clone of Trmt1 (Myc-DDK-tagged) - Mouse TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) (Trmt1), transcript variant 3 |
CNY 8,460.00 |
|
MR224696L4 | Lenti ORF clone of Trmt1 (mGFP-tagged) - Mouse TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) (Trmt1), transcript variant 3 |
CNY 8,460.00 |