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Dspp (NM_010080) Mouse Tagged ORF Clone
CAT＃: MG224702
- TrueORF®
Dspp (tGFP-tagged) - Mouse dentin sialophosphoprotein (Dspp), (10ug). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_010080" in other vectors (2)
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CNY 9,220.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | D; Dmp; Dmp2; Dmp3; Dpp; Dsp |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224702 representing NM_010080, codon optimized.
Due to the complexity of NM_010080, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAATGAAAATCATTATCTATATTTGTATCTGGGCAACCGCTTGGGCGATCCCGGTCCCCCAGCTTG TACCCCTGGAGCGCGACATTGTCGAAAACTCCGTGGCCGTACCACTTCTGACCCACCCAGGAACGGCCGC ACAGAATGAGCTTTCCATAAATTCCACCACTAGCAATAGCAACGATTCCCCAGATGGTAGTGAGATTGGT GAACAGGTACTTTCCGAGGACGGCTACAAGAGGGACGGAAACGGATCTGAGTCCATACACGTGGGGGGTA AGGACTTTCCAACCCAGCCTATCCTGGTGAATGAACAGGGGAATACGGCAGAAGAACACAACGATATTGA GACGTATGGGCATGACGGAGTCCACGCTCGCGGCGAGAATTCCACAGCGAACGGTATACGGTCCCAGGTC GGAATTGTTGAGAATGCTGAAGAAGCCGAATCCTCCGTGCATGGGCAGGCAGGTCAAAACACCAAAAGCG GAGGAGCTAGTGATGTATCCCAGAACGGAGACGCAACGCTTGTTCAGGAGAACGAACCCCCAGAGGCCAG CATAAAGAATTCCACAAACCACGAGGCTGGGATCCACGGAAGCGGTGTCGCAACACACGAGACTACGCCC CAGCGCGAAGGACTTGGGTCTGAGAATCAGGGGACTGAGGTTACCCCAAGTATCGGCGAGGACGCGGGTC TGGATGATACCGATGGGAGCCCTTCCGGCAACGGGGTGGAGGAGGATGAAGATACGGGCTCTGGCGATGG GGAGGGTGCCGAAGCGGGCGACGGAAGGGAATCTCACGACGGCACTAAAGGCCAAGGTGGGCAGTCCCAC GGGGGCAATACCGATCACCGCGGGCAGAGTAGTGTATCTACTGAGGATGACGACTCTAAAGAGCAGGAGG GTTTCCCGAATGGGCATAACGGCGACAATAGCTCCGAGGAGAATGGGGTGGAGGAAGGGGATAGCACTCA GGCCACCCAAGATAACCAAAAGCTGAGTCCCAAAGATACTCGAGATGCCGAGGGGGGGATCATCAGTCAG AGCGAAGCCTGTCCGTCTGGGAAATCTCAGGATCAAGGCATTGAGACAGAGGGTCCGAATAAGGGCAACA AGAGCATTATTACCAAAGAAAGTGGCAAGCTCTCCGGAAGTAAAGATAGCAATGGCCATCAGGGCGTTGA GCTGGACAAACGCAACAGCCCTAAGCAGGGGGAGTCCGACAAACCGCAGGGGACCGCGGAAAAGTCAGCT GCTCACAGCAATCTCGGCCACAGCAGGATAGGATCAAGCTCAAATAGTGATGGGCACGATTCCTATGAAT TCGACGACGAGTCTATGCAGGGGGACGATCCTAAATCATCCGATGAAAGTAACGGCAGCGACGAATCCGA CACTAACAGTGAATCAGCCAACGAGAGCGGATCTAGAGGGGACGCTTCATACACTTCCGATGAATCATCT GACGACGATAACGATTCTGATAGTCATGCTGGCGAAGATGATTCATCTGATGATTCCAGCGACACTGACG ATAGCGACTCCAACGGGGACGGGGACAGCGATAGTAACGGGGACGGAGACAGCGAGAGCGAAGATAAGGA TGAGTCCGATTCATCCGACCATGACAACTCCAGTGATAGTGAGTCCAAGTCCGACTCTTCCGATTCCAGC GACGATAGCTCAGATTCTTCCGACTCCTCTGATAGTAGCGATTCTTCTGACTCCTCAGATAGCTCTGACA GCAGCGACTCCAGTGACAGCTCCGATTCTAACAGCTCCAGCGACTCCTCCGACAGCAGTTCTTCAAGTGA TTCATCTGATTCCTCAGATAGCAGTGACAGCTCTGATAGCTCAGACAGCTCTGATAGCAGCGACTCTAGC GATTCCAGCGATTCATCTGACAGTAGTGACTCCAGCGACTCATCCGACAGTAGTGACTCCTCCAGCTCAT CAGACTCATCTGATTCCAGTAGTTCTTCAGATTCCTCCGACAGTAGCGATAGCTCCGATTCTAGTGACTC CTCCGACTCCAGCGACTCCAGTTCAAGTGACAGCAGTAGCAGCTCCAATAGTAGCGACTCCAGTGACTCC AGTGACTCCTCATCAAGCAGCGACTCTTCCAATTCATCCGACTCTTCCGACAGTTCAGATAGTAGCGATT CCTCCGACTCCTCAGACAGCTCCAATTCTAGCGATAGTTCCGATTCCTCCAGCAGCTCTGACTCCTCAGA TTCCTCTTCCTCCTCCGATTCTTCCGATAGTAGTGATAGTTCTGACAGCTCCGAGTCCTCTGAGAGTAGT GATTCTTCTAATAGCAGCGACTCCAGTGACTCTTCCGATTCTTCTGATTCAAGCGATTCTAGCGACAGTA GCGACTCTAGCGACTCCAGCGACAGTTCAAACAGTTCCGACAGTAGCGATAGCAGCGACTCTTCCGATAG CAGTGACTCTAGTAACTCTTCAGACTCAAGTGATAGTTCTGACTCAAGCGATTCAAGCGACAGTTCAGAT AGCTCTGATAGTTCTGACTCCAGTGATAGTAGCGACTCATCCGACAGCTCTGATTCATCTGATAGCTCCG ACTCCTCCGATAGTTCAGACAGTAGCGATAGTAGCGACTCAAGCGACAGCAGCGATTCCTCCGACTCCTC AGATAGTAGCAACAGTTCCGACTCTTCTGACAGCGATTCCAAGGATTCTTCTAGTGATTCTTCTGACGGG GACAGTAAAAGTGGCAATGGCAACAGCGATTCCAATAGTGACTCAAATTCCGACTCTGACTCCGACAGCG AGGGGAGCGACTCTAATCACAGCACGTCCGACGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224702 representing NM_010080
Red=Cloning site Green=Tags(s) MKMKIIIYICIWATAWAIPVPQLVPLERDIVENSVAVPLLTHPGTAAQNELSINSTTSNSNDSPDGSEIG EQVLSEDGYKRDGNGSESIHVGGKDFPTQPILVNEQGNTAEEHNDIETYGHDGVHARGENSTANGIRSQV GIVENAEEAESSVHGQAGQNTKSGGASDVSQNGDATLVQENEPPEASIKNSTNHEAGIHGSGVATHETTP QREGLGSENQGTEVTPSIGEDAGLDDTDGSPSGNGVEEDEDTGSGDGEGAEAGDGRESHDGTKGQGGQSH GGNTDHRGQSSVSTEDDDSKEQEGFPNGHNGDNSSEENGVEEGDSTQATQDNQKLSPKDTRDAEGGIISQ SEACPSGKSQDQGIETEGPNKGNKSIITKESGKLSGSKDSNGHQGVELDKRNSPKQGESDKPQGTAEKSA AHSNLGHSRIGSSSNSDGHDSYEFDDESMQGDDPKSSDESNGSDESDTNSESANESGSRGDASYTSDESS DDDNDSDSHAGEDDSSDDSSDTDDSDSNGDGDSDSNGDGDSESEDKDESDSSDHDNSSDSESKSDSSDSS DDSSDSSDSSDSSDSSDSSDSSDSSDSSDSSDSNSSSDSSDSSSSSDSSDSSDSSDSSDSSDSSDSSDSS DSSDSSDSSDSSDSSDSSDSSSSSDSSDSSSSSDSSDSSDSSDSSDSSDSSDSSSSDSSSSSNSSDSSDS SDSSSSSDSSNSSDSSDSSDSSDSSDSSDSSNSSDSSDSSSSSDSSDSSSSSDSSDSSDSSDSSESSESS DSSNSSDSSDSSDSSDSSDSSDSSDSSDSSDSSNSSDSSDSSDSSDSSDSSNSSDSSDSSDSSDSSDSSD SSDSSDSSDSSDSSDSSDSSDSSDSSDSSDSSDSSDSSDSSDSSDSSDSSNSSDSSDSDSKDSSSDSSDG DSKSGNGNSDSNSDSNSDSDSDSEGSDSNHSTSDD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010080 |
| ORF Size | 2835 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010080.2 |
| RefSeq Size | 4440 bp |
| RefSeq ORF | 2838 bp |
| Locus ID | 666279 |
| Gene Summary | This gene encodes a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of proteins. The encoded preproprotein is secreted by odontoblasts and proteolytically processed to generate two principal proteins of the dentin extracellular matrix of the tooth, dentin sialoprotein and dentin phosphoprotein. These two protein products may play distinct but related roles in dentin mineralization. Mice lacking the encoded protein exhibit hypomineralization defects in dentin, similar to human dentinogenesis imperfecta. [provided by RefSeq, Feb 2016] |
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