Papola (NM_011112) Mouse Tagged ORF Clone
CAT#: MG224835
- TrueORF®
Papola (tGFP-tagged) - Mouse poly (A) polymerase alpha (Papola), (10ug)
ORF Plasmid: DDK
"NM_011112" in other vectors (4)
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CNY 6,370.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Pap; PapIII; Plap |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224835 representing NM_011112
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGTTTCCAGTTACAACGCAGGGATCACAACAAACGCAGCCACCACAGAGGCACTATGGCATTACCT CTCCTATCAGCTTAGCGGCCCCCAAGGAGACTGACTGCCTACTCACACAGAAGCTCATCGAGACGCTGAA GCCCTTTGGGGTTTTTGAAGAAGAAGAGGAACTGCAGCGCAGGATTTTAATTTTGGGAAAATTAAATAAC CTGGTGAAAGAATGGATTCGAGAAATCAGTGAAAGCAAGAATCTCCCACAATCTGTAATTGAAAATGTTG GAGGGAAGATTTTTACATTTGGATCTTACAGACTAGGAGTCCACACGAAAGGTGCTGATATTGATGCGTT GTGTGTTGCACCAAGACATGTTGATCGAAGTGACTTTTTCACCTCATTCTATGATAAATTGAAATTACAA GAAGAAGTGAAAGATTTAAGAGCTGTTGAAGAGGCATTTGTACCAGTTATCAAACTCTGTTTTGATGGAA TAGAGATTGATATTTTGTTTGCAAGATTAGCACTGCAGACTATTCCAGAAGATTTGGACCTACGAGATGA CAGTCTGCTTAAAAACCTAGATATAAGATGCATAAGAAGCCTTAATGGTTGCAGGGTAACCGATGAAATT TTACATCTAGTACCAAACATTGACAACTTCAGGTTAACTCTGAGAGCCATCAAACTGTGGGCCAAACGGC ACAACATCTATTCCAATATATTAGGTTTCCTCGGTGGTGTTTCCTGGGCTATGCTAGTAGCAAGAACTTG CCAGCTTTATCCAAATGCAATAGCATCAACTCTTGTACATAAATTTTTCTTGGTATTTTCTAAATGGGAA TGGCCAAATCCAGTGCTATTGAAACAGCCTGAAGAATGCAATCTTAATTTGCCTGTGTGGGACCCAAGGG TAAACCCCAGTGATAGGTACCATCTTATGCCTATAATTACACCAGCATACCCACAGCAGAACTCCACGTA CAATGTGTCCGTTTCAACACGGATGGTCATGGTTGAGGAGTTTAAACAAGGTCTTGCTATCACAGATGAA ATTTTGCTGAGTAAGGCAGAGTGGTCCAAACTTTTTGAAGCTCCAAACTTCTTTCAGAAGTACAAGCATT ATATTGTACTTCTAGCAAGTGCGCCCACGGAAAAGCAGCGTCTGGAATGGGTGGGCTTGGTGGAATCAAA AATCCGCATCCTGGTTGGAAGCTTGGAGAAGAATGAGTTTATTACACTGGCTCATGTGAATCCCCAGTCA TTTCCAGCCCCCAAAGAAAGTCCTGACAGGGAAGAATTTCGCACAATGTGGGTGATTGGGTTAGTGTTTA AAAAAACTGAAAACTCTGAAAATCTCAGTGTCGACCTCACCTATGATATCCAGTCTTTCACAGACACAGT TTATAGGCAAGCAATAAACAGCAAAATGTTTGAGTTGGATATGAAGATTGCAGCAATGCATGTGAAGAGA AAGCAACTCCATCAGCTGCTGCCTAGTCACGTGCTTCAGAAGAGGAAGAAGCATTCAACAGAAGGAGTCA AGTTAACAGCTCTGAATGACAGCAGCCTTGACTTGTCTATGGACAGTGATAACAGCATGTCTGTGCCTTC ACCCACCAGTGCTATGAAGACCAGTCCATTGAATAGTTCTGGCAGCTCCCAGGGCAGAAACAGTCCTGCT CCAGCTGTGACCGCAGCATCTGTGACCAGCATCCAGGCTTCTGAGGTTTCTGTACCGCAAGCAAATTCCA GTGAAAGCCCAGGGGGTCCATCGAGCGAAAGCATTCCTCAAACTGCCACACAGCCAGCCATTTCTCCACC ACCAAAGCCTACAGTCTCCAGAGTTGTCTCCTCAACACGACTGGTAAACCCATCGCCTAGACCTTCAGGA AACACAGCAACAAAAGTCCCTAATCCTATAGTAGGAGTCAAGAGAACGTCCTCACCCAATAAAGAAGAAA GTCCTAAGAAAACCAAAACAGAAGAGGATGAAACAAGTGAAGATGCTAACTGTCTTGCTTTGAGTGGACA TGATAAAACAGAGACAAAGGAACAAGTTGATCTGGAGACAAGTGCGGTTCAATCAGAAACTGTTCCGGCA TCGGCTTCTCTGTTGGCCTCTCAGAAAACATCCAGTACAGACCTTTCTGATATCCCTGCTCTCCCTGCAA ATCCTATTCCTGTTATCAAGAATTCAATAAAACTGAGACTGAATCGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224835 representing NM_011112
Red=Cloning site Green=Tags(s) MPFPVTTQGSQQTQPPQRHYGITSPISLAAPKETDCLLTQKLIETLKPFGVFEEEEELQRRILILGKLNN LVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQ EEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEI LHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWE WPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDE ILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQS FPAPKESPDREEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFELDMKIAAMHVKR KQLHQLLPSHVLQKRKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSAMKTSPLNSSGSSQGRNSPA PAVTAASVTSIQASEVSVPQANSSESPGGPSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPSPRPSG NTATKVPNPIVGVKRTSSPNKEESPKKTKTEEDETSEDANCLALSGHDKTETKEQVDLETSAVQSETVPA SASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011112 |
ORF Size | 2217 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011112.4 |
RefSeq Size | 4518 bp |
RefSeq ORF | 2220 bp |
Locus ID | 18789 |
UniProt ID | Q61183 |
Gene Summary | Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221226 | Papola (untagged) - Mouse poly (A) polymerase alpha (Papola), (10ug) |
CNY 9,600.00 |
|
MR224835 | Papola (Myc-DDK-tagged) - Mouse poly (A) polymerase alpha (Papola) |
CNY 5,416.00 |
|
MR224835L3 | Lenti ORF clone of Papola (Myc-DDK-tagged) - Mouse poly (A) polymerase alpha (Papola) |
CNY 7,700.00 |
|
MR224835L4 | Lenti ORF clone of Papola (mGFP-tagged) - Mouse poly (A) polymerase alpha (Papola) |
CNY 7,700.00 |