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Aff4 (NM_033565) Mouse Tagged ORF Clone
CAT＃: MG224878
- TrueORF®
Aff4 (tGFP-tagged) - Mouse AF4/FMR2 family member 4 (Aff4), (10ug)
ORF Plasmid: DDK
"NM_033565" in other vectors (4)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AF5Q31; Alf4; Laf4l; MCEF |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224878 representing NM_033565
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCGTGAAGACCGGAATGTGCTGCGCATGAAAGAAAGGGAAAGGCGGAATCAGGAAATTCAGCAGG GTGAAGACGCCTTCCCACCTAGTTCTCCTCTCTTTGCTGAGCCATACAAAGTCACTAGCAAAGAAGATAA GTTATCAAGTCGGATTCAGAGCATGCTTGGAAACTACGATGAAATGAAGGATTACATAGGAGACAGATCG ATACCAAAGCTTGTTGCAATTCCCAAGCCTGCAGTACCAACGACCACAGACGAGAAAGCAAACCCAAATT TTTTTGAGCAGAGACATGGAGGATCTCATCAGAGTAGCAAATGGACTCCTGTAGGCCCCGCCCCCAGCAC CTCTCAGTCTCAGAAACGCTCCTCTGCCCTGCAGAGTGGACACAGCAGCCAACGGAGTGGTGCAGGAGGG AGTGGCGCCAGCAGCAGTGGCCAGAGGCATGACCGTGACTCCTACAGCAGTAGCAGGAAAAAAGGCCAGC ACGGCTCAGAGCACTCCAAATCTCGATCCTCAAGCCCTGGAAAACCCCAGGCTGTTTCTTCATTAAGTTC TAGTCATTCCAGGTCTCATGGGAATGATCACCATAGCAAGGAACATCAGCGCTCCAAATCACCTCGGGAC CCTGATGCCAACTGGGATTCTCCTTCCCGCGGACCTTTTTCAAGTGGGCAACATTCAAGTCAGTCTTTCC CACCTTCTTTGATGTCAAAATCTAGTTCAATGTTACAGAAACCCACTGCCTATGTACGGCCCATGGATGG ACAGGAGTCTGTGGAACCAAAGCTGTCCTCTGAGCACTACAGCAGCCAGTCTCATGGTAATAGCATGACT GAGCTGAAGCCCAGCAGCAAAGCACATCTCACCAAGCTGAAAATTCCTTCCCGACCTTTGGATGCATCGG TTTCTGGTGATGTAAGCTGTGTAGATGAAATACTTAAAGAGATGACGCATTCATGGCCTCCCCCTCTAAC GGCTATTCATACACCATGCAAAACAGAACCTTCCAAATTCCCTTTTCCAACTAAGGAGTCGCAACAGTCT AACTTTGGCCCTGGAGAACAAAAAAGATACAGTACTGCTAAAACATCAAATGGACACCAGTCAAAATCTA TGTTAAAAGATGACTTAAAACTAAGCAGCAGTGAAGACAGTGATGGGGAACAGGATTGTGATAAGACAAT GCCAAGGAGTACACCAGGAAGTAACTCTGAACCTTCACACCATAACAGTGAGGGAGCAGACAACTCCAGG GATGACTCGAGCAGCCACAGTGGATCTGAGAGCAGCTCTGGGTCTGACTCGGAGAGTGAGAGCAGCTCCA GTGACAGTGAGGCTAATGAGCCGTCGCAGAGCGCATCTCCTGAGCCTGAACCACCACCAACAAACAAGTG GCAACTTGATAATTGGTTGAATAAAGTGAACCCACATAAAGTATCCCCAGCTTCCTCTGTAGACAGCAAC ATCCCATCTTCTCAAGCCTACAAGAAAGAAGGCCGGGAGCAGGGCACTGCCAGTAACTACACAGATCCAG GGGGGACAAAAGAAACGAGCTCTGCCACCCCAGGACGAGACTCTAAAACCATCCAAAAGGGATCAGAAAG TGGCCGTGGGAGGCAGAAGTCTCCTGCACAGAGCGACAGTACCACTCAGAGGAGAACTGTCGGCAAAAAA CAACCCAAAAAACCTGAGAAGTCAGCTGCTGAAGAGCCTCGTGGAGGCCTGAAGATAGAAAGTGAGACCC CTGTGGACATGGCTGCCAGCATGCCCTCCAGCAGGCACAAAGCAGCCACCAAGGGCTCGAGGAAACCCAA CATCAAAAAGGAATCAAAATCTTCTCCTCGACCTACGGCAGAGAAGAAGAAGTACAAGTCAGCCAGTAAA CCGTCCCAGAAGTCTCGGGAGATCATAGAGACTGACACCTCGTCCTCAGACTCGGACGGCAGCGAGAGCC TGCCTCCCTCCTCACAGACTCCTAAGTACCCTGAAAGCAACAGGACTCCTGTCAAACCCTCCTCAGTGGA GGAAGAAGATAGCTTTTTTCGGCAACGAATGTTCTCTCCTATGGAAGAGAAGGAGCTCCTTTCCCCACTC AGTGAGCCTGATGACAGGTATCCACTCATTGTGAAGATTGACCTCAATCTTCTGACTCGAATACCCGGGA AGCCTTACAAAGAAACAGAGCCACCCAAGGGGGAAAAGAAAAATGTTCCAGAGAAACACTCGAGAGAGGT GCAGAAGCAAGCCTCAGAAAAGGCTTCCAACAAAGGCAAGAGGAAACACAAGAATGATGATGATACCCGA GCCAGTGAGAGCAAAAAACCCAAAACAGAGGACAAGAACTCATCAGGCCACAAGCCATCCAGCAGCAGAG AGTCATCTAAGCAGAGTAGTACAAAAGAAAAGGACTTGCTACCTTCACCTGCTGGGCCCATTCTTTCAAA AGATTCAAAAACAGAGCATGGTTCTCGGAAGAGGACTGTCAGTCAGTCGTCGTCCTTAAAGTCCAGTGGG ACCAGCAGCAAAGAGAACAGTGGCAGCAGCAGCAAGAGCAGCTCCTCCTCCACAGCGAAGCAGAAGAAGA CTGAAGGCAAGGGTCCCAGCAGCTCCAAGGAGGCCAAGGAGAAGGCTCCAAATAGCTCCTCTAATTGTCC TCCGTCTACACCAACTTCTGAGTCTTCGAAGCCTCGGAGAACAAAGCTTGCCTTTGATGACAGAAATTAT TCAGCAGACCATTATTTACAAGAAGCAAAAAAACTCAAACACAATGCAGATGCTTTGTCTGACAGGTTTG AGAAAGCCGTGTACTACCTTGATGCTGTAGTGTCTTTCATTGAGTGTGGGAATGCATTAGAGAAGAACGC TCAGGAATCCAAGTCCCCATTCCCTATGTACTCAGACACGGTGGAGCTCATTAAGTACACTATGAAACTA AAGAATTATTTGGCACCAGATGCTACAGCTGCAGACAAGAGACTCACAGTACTTTGCCTGAGATGCCAGT CATTATTGTACCTGAGGCTGTTCAAACTGAAGAAGGAGAACGCTCTCAAGTACTCCAAGACGCTAACTGA GCACCTGAAGAATTCTTACAGTAATTCTCAAGCACCTTCACCCGGCTTGGGGAGCAAAGCTGTTGGGATG CCCTCCCCTGTTTCTCCAAAACTGTCACCAGGCAATTCTGGAAGTTACTCTTCTGGGGGAAGTAGTGCTT CAGCAAGTGGTTCTTCAGTGACCATTCCACAGAAAATCCACCAGATGGCAGCAAGCTATGTCCAGGTTAC ATCTAACTTCCTCTATGCCACAGAAATTTGGGACCAAGCTGAACAGCTTTCCAAAGAACAAAAAGAATTC TTTGCTGAACTGGACAAAGTGATGGGCCCTCTCATCTTTAATGCAAGCATCATGACAGATCTGGCTCGTT ACACCCGGCAGGGACTGCATTGGCTTCGGCAGGATGCCAAGCTGATATCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224878 representing NM_033565
Red=Cloning site Green=Tags(s) MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDYIGDRS IPKLVAIPKPAVPTTTDEKANPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSALQSGHSSQRSGAGG SGASSSGQRHDRDSYSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLSSSHSRSHGNDHHSKEHQRSKSPRD PDANWDSPSRGPFSSGQHSSQSFPPSLMSKSSSMLQKPTAYVRPMDGQESVEPKLSSEHYSSQSHGNSMT ELKPSSKAHLTKLKIPSRPLDASVSGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQS NFGPGEQKRYSTAKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSR DDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSN IPSSQAYKKEGREQGTASNYTDPGGTKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKK QPKKPEKSAAEEPRGGLKIESETPVDMAASMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSASK PSQKSREIIETDTSSSDSDGSESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPL SEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHSREVQKQASEKASNKGKRKHKNDDDTR ASESKKPKTEDKNSSGHKPSSSRESSKQSSTKEKDLLPSPAGPILSKDSKTEHGSRKRTVSQSSSLKSSG TSSKENSGSSSKSSSSSTAKQKKTEGKGPSSSKEAKEKAPNSSSNCPPSTPTSESSKPRRTKLAFDDRNY SADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSDTVELIKYTMKL KNYLAPDATAADKRLTVLCLRCQSLLYLRLFKLKKENALKYSKTLTEHLKNSYSNSQAPSPGLGSKAVGM PSPVSPKLSPGNSGSYSSGGSSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEF FAELDKVMGPLIFNASIMTDLARYTRQGLHWLRQDAKLIS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033565 |
| ORF Size | 3480 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033565.2, NP_291043.1 |
| RefSeq Size | 10165 bp |
| RefSeq ORF | 3483 bp |
| Locus ID | 93736 |
| UniProt ID | Q9ESC8 |
| Gene Summary | Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223774 | Aff4 (untagged) - Mouse AF4/FMR2 family, member 4 (Aff4), (10ug) |
CNY 14,920.00 |
|
| MR224878 | Aff4 (Myc-DDK-tagged) - Mouse AF4/FMR2 family, member 4 (Aff4) |
CNY 8,088.00 |
|
| MR224878L3 | Lenti ORF clone of Aff4 (Myc-DDK-tagged) - Mouse AF4/FMR2 family, member 4 (Aff4) |
CNY 10,260.00 |
|
| MR224878L4 | Lenti ORF clone of Aff4 (mGFP-tagged) - Mouse AF4/FMR2 family, member 4 (Aff4) |
CNY 10,260.00 |
