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Dido1 (NM_175551) Mouse Tagged ORF Clone
CAT＃: MG225049
- TrueORF®
Dido1 (tGFP-tagged) - Mouse death inducer-obliterator 1 (Dido1) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_175551" in other vectors (2)
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CNY 24,800.00
货期*
9周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6720461J16Rik; C130092D22Rik; D130048F08Rik; Datf; DATF-1; Datf1; di; dido; DIO; DIO-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225049 representing NM_175551
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGATAAAGGGCACCTGAGCAATGAGGAAGCACCCAAGGCTATCAAACCCACCAGTAAGGAGTTCA GGAAAACCTGGGGTTTTCGAAGAACCACGATTGCCAAACGTGAGGGTGCAGGAGACACGGAGGTGGACCC CAGTGAGCAGCAACCACAGCAGCATAACCTCTCCCTGCGCCGCAGTGGACGGCAACCAAAACGTACTGAG AGGGTAGAAGAGTTTCTTACCACGGTTCGGCGCCGAGGGAAAAAGAATGTGCCGGTGTCCCTGGAGGATT CCAGTGAGCCCACATCTTCCACAGTCACTGATGTGGAGACAGCTTCCGAGGGGAGCGTTGAAAGCAGTTC TGAGATCAGAAGTGGCCCTGTATCTGACTCCTTAGGGAAAGAACATCCTGCCTCTTCTGAAAAGGCAAAA GGAGGTGAAGAGGAAGAAGACACCTCTGACAGTGACAGTGATGGCCTTACGTTGAAGGAACTTCAGAACC GCCTTCGGAGAAAGCGAGAGCAAGAACCTGTGGAGAGGTCCCTGAGAGGCAGTCAGAATCGCCTGAGGAA GAAGCGCAGAGAGGAAGATTCTGCCGAAACTGGGAGTGTCCAAATAGGCAGTGCCGAGCAGGACAGACCT CTCTGTAAGCAGGAGCCTGAGGCTAGTCAGGGACCAGTGTCCCAGTCAGAGACAGATGACATAGAAAATC AGTTGGAAGGGAAGGCGACTCAGGGAAATACAGAGGAAAACCCCAGGGAAGCGGGCAAACCAAAGCCTGA GTGTGAGGTTTACGACCCCAATGCCCTGTACTGCATCTGCCGCCAGCCTCACAACAACAGGTTTATGATC TGCTGTGATCGGTGTGAGGAGTGGTTCCATGGTGACTGTGTGGGTATTTCTGAGGCCCGAGGGCGGCTCC TGGAAAGGAACGGGGAAGACTACATCTGCCCAAATTGCACCATTTTGCAAGTGCAGGATGAGACAAACGG TAGCGCCACCGATGAGCAGGACTCTGGGTGCAGATCTGTGGGTGCTGATGGCACAGACTGCACAAGCATA GGGACAGTAGAGCAGAAGTCCGGAGAAGACCAGGGCATAAAGGGTAGGATTGAGAAGGCAGCAAACCCCA GCGGCAAGAAAAAACTCAAGATATTCCAGCCTGTCGTAGAGGCTCCTGGTGCTCCTAAATGCATTGGCCC TGGGTGTTCCAGTGTAGCACAGCCTGACTCTGTGTATTGCAGTAATGACTGCATTCTCAAACACGCAGCA GCTACCATGAGATTTCTAAGTTCAGGTAAAGAACAAAAAACAAAACCCAAGGAAAAGGTCAAGACGAAGC CAGAAAAGTTCAGTCTTCCAAAATGCAGTGTTCAGGTGGGGATTAAAATCTCTTCTGTGCACAAGAGACT AGCGTCAGAGAAAAGGGAAAACCCAGTGAAGAAAGTGATGCTGGCTTCCAGGAGTGAGACTTCTGGGAAG GAGGCAGCCTGTGAGAGCAGCACACCATCCTGGGCAAGTGACCACAACTACAATGCTGTGAAGCCAGAGA AGCCAGAGAAGCCCACTGCACTCTCGCCCACCCTATTGAGTAAATCCATGAAGGATGACAGGAGGGTGGA GGACAGGACAATGGCAGCAGTTACCATCCCGAAGAAAGCACTTCCTTCAGCCTCTTTGGTGGGCAGACAG ACTTCACCTAGGAATCTTGTTCCAAAGAAGCTTCCTCCTTACTCTAATATGGCAGGAGCCAAACCAGCCA TTAAGAAACTGCCTTCAGGCTTCAAGGGTACCATCCCCAAGAGGCCATGGCCCTCAGCCACCCTGTCAGG CACTTCTGCCAGGCAGGCAGGACCAACACCTATGACAGCTGCTTCCAAAAAGTTGCCTGGCTCTGCTGCT GTGGTGGGAGTTACCAGGAAGCCAATGTCTGCCAATGTTCCTGCTGCTTCTCCAGCCCCAGGACGGCTTG GTCCTGTGAGCCCAGCTCCATCACAGCCCAATTCACAAATTCGACAAAATATAAGGCGCTCCTTGAAAGA GATTTTGTGGAAAAGAGTCAATGACAGCGATGACTTAATAATGACAGAAAATGAAGTAGGAAAAATTGCC CTCCACATTGAGAAGGAGATGTTTAACTTGTTCCAGGTTACTGATAATCGCTATAAGAGCAAATATCGCA GCATCATGTTCAACCTTAAGGATCCTAAGAATCAGGGGCTCTTCCATCGTGTTCTTCGAGAAGAAATCTC CTTGGCAAAACTTGTCAGAATGAAGCCTGAAGAACTTGTATCTAAAGAGCTTTCTATGTGGACAGAGAAG CCCACAAAATCTGTGATAGAATCCAGGACTAAGTTGCTTAATGAAAGCAAGAAGAACACTACTAAACCAG AAACCATTCCTGACATGGAAGATTCTCCACCGGTATCAGATTCAGAAGAACAACAAGAGTCAGTGCGAGC CGCCCCTGAGAAGAGCGCAGCACCTCTCCTGGACGTCTTCAGCAGCATGCTGAAGGACACCACAAGCCAG CACCGTGCCCATCTTTTTGATTTAAACTGTAAAATCTGTACAGGTCAGGTTCCATCCTCGGAGGATGAAC CAGCTCCTAAGAAGCAAAAGCTTTCAGCTTCTTCTAAGAAGGAAGACTTTAAGCCCAGGCATGACAGCTC TCCACCTAATGCAGTTCCTAACACTGCTGATGAAGGGATTGCAGACACGCTGCCTGAAAATGCCTCTGAG CCAGACCCGGAGAGTACATCTAGTCTTAACCAGGAGAGAAAGTGTTTCCCTGAGTCTCCAGGCGATAGCC ATCCTGAGCCCTCGTCTCTGGGTGGCCTTTCTCCCTCTTCTGCCTCTGGTGGGAGTGGGGTGGTCACCAC AGTCACCATGTCTGGTCGAGACCCCAGAACTGCCCTGAGTGGGTCGTGCACAGTCACAGCCTCCATGGCA GCCCATCTGGACAACTCCCAGGCTTCAGAAACCAAACTGGACATGATAAAGCCTGCATTGACTTCTGCAG TGGTGCCCAAGTCCATACTGGCTAAGCCATCCTCCTCTCCTGACCCGAGATACCTGTCAGTACCACCATC ACCAAGCATTTCAGAATCACGATCCCCACCAGAAGGAGATACAACCCTCTTTTTGTCTCGACTCAACACG ATTTGGAAAGGATTTATTAATATGCAGAGTGTAGCAAAATTTGTCACTAAGGCATATCCTGTCTCTGGGT GTTTGGATTATCTCAGTGAGGATTTGCCAGACACCATCCACATCGGTGGAAGGATTGCTCCAAAGACCGT GTGGGATTACGTTGGCAAACTCAAGTCATCGGTGTCCAAGGAGCTCTGTCTGATCCGCTTCCACCCTGCC ACTGAGGAGGAGGAGGTTGCATACATTTCTCTCTACTCCTATTTTAGCAGCCGTGGTCGCTTTGGGGTTG TAGCTAATAACAACAGGCATGTCAAGGACCTGTACCTGATTCCACTGAGTGCTAAGGACCCTGTGCCATC CAAACTCTTGCCCTTTGAGGGCCCAGGTCTTGAATCACCACGTCCAAATATAATACTTGGGTTAGTAATA TGTCAGAAAGTGAAACGTCCTTCAAGTGCTGGCGAGTTAGACAAGACAGATGAGAAGCGGACTCGACTCC AACAGGAAGAACTAGAAACTTCAGTCTATCCCAAAGTAACTGCAGCTTTGCCATCCGAGAAGAAGCCTCC CAAGTACAGTGTACACTCTATAGACACAGCTGCTACCAGCACCACGCCCCCTGGCTCTCCTCCCCCACCT CCTCCTCTTCCTGAACCCCCAGTATTGAAAATACTGTCATCCCTCAAGCCAGGGTCCACCAGCACTGTCA CAGCGCCTACCACAGCAGCAATCACTACCACAGCTTCCCCTGTTACTGCTGCCACTTCCAAAACAGCCTC GCCCCTGGAACACATCCTGCAGACTCTCTTTGGGAAAAAGAAATCATTTGAGCCGTCTGGAAAAGAATCT GTTGGGTCTACTCTATCTCCACATCAGGATTCCAAGGCCAAGGGGGAGGACACCATGTCAGCAGCTCCTT TGTTGGATCCAATTGTTCAACAGTTTGGTCAGTTCTCAAAAGACAAGGCTCTGGAGGAGGAGGAGGAAGA TGACAGGCCCTACGACCCTGAAGAAGAGTACAACCCTGATCGAGCATTTCATACACTGCTCGCTGAGCCA GGGAGGCCTCATGATGTGCAGAGTGTGTCTGAGACAGCTGAGAGGGAGGAGGTGGCCTATGACCCAGAAG ATGAGACCATCTTAGAAGAAGCCAAAGTGACCATTGATGACTTGCCCAATCGCATGTGTATGAAAGTTAG CGCCACAGAAAGGCCTGCCGACTTCACCACCGATGCCTCCAGTGCTTCGCTGGTAGAGCAGCAGAAAATG TTAGAAGAGCTAAATAAACAGATTGAGGAGCAAAAGAGGCAGCTGGAGGAGCAAGAAGAAGCTCTCCGAC AGCAGAGGGCTGCTGTGGGTGTGTCCATGGCACACTTTTCTGTGTCCGATGCATTGATGTCACCACCTCC CAAGTCGTCTTTGGGCAAGACAGAGCTGTTCTCTCAAGAACAGCAAGCCCCAGACCCAAGTCAGGGAGCC CCGAACACCAACCACAACCTAGACTCAAGACAAAGTAGGGACCCAAGGCAGGCCAGGCGGCTCGCTGCAG AAAACACAGAGAATGAATCCCTTCCTAGGGCCCCCACAGGAAGCACACCTGGCCCACAAGGCACCCTACC TGCAAGGGAGACTCCTGCTGGGACTGCAGTAGTCCAGGGGCCTGGGCTGGCAGCAGAAGCCAAGGAGTCA ATGGCTGTTCCCTGGGCTCCAGGTGAAAACGCTGTGTTGAGACCTGAACATGACATCCAAAAATGTGAGC ACCCTGGTAACCCTGTCTCATTACCCCTGGACACCAGCCACCTTCCTACAGCTGGGGATGGCGCAGCCAG GCCAGCACCGCCCCGCAGAGTGCTGCTACCCACACCCCCTAGCACCACCTTTCCACCCAGCTTCCCTTTG CAACCTAAGGCCCAAAATTTTAGTTCTGGAAGCAGGGAGCCTTTCTCAGGGCCCACATTTATGTCTCAGG AAACATCTCTTGGCTCATCCCAATATGAGGACCCCAGGGGTGCTCAGTCTGCTGGGAAGAATGACAGCCC AGTAGCTGATATGGAGGACAGCAGAGAGCCACAGCCTAGGCCTGGTGAGAGTACTACTTCATTCCCCCAA CCTGGGCAGAGGGGAGGGGGCCCTCAGCCTCAGTTTCCTGGCCAGCGTGAACCTGCACCACGCACTTTTG GGATGTCGGGTCATCATGGTCCCAGTTTTCCAGGACCTAGGGGGCCAGTCCCTCCTTATTCAGAAGAGAA CCTTGTTCCTAATAGTGATGGACCAAGAGGCCCTCCACCTGCCAGATTTGGGGCACAGAAGCCACCAATC CCTTCCTTATTCTCTGGGCAACATGGGCCACCTCCCTATGGGGACAACAGGGGACTCTCACCTTCTTATC TTGGTGGGCCCCGAGGAGGAGCACCAGCCCAATTTGAAGACCGCAAGGATCCTCATGGGGAGAAAAGGGA ATTCCAGGACACTCCGTACAACGAGATGACAGGTGCCCCTGCTCAGTGTGAAGGGCCAGATCAGGCCCAG TTCATGGGGAACAGGGCACCCTTTCAGTTTGGAGGCCAGAGACGGCCACTGCTAACTCAAATGAAAGGGC CCCGTGGAGGACCTCCACCCTCTCAGTTTGGAGCCCAAAGGGGACCACCTCCTGGTCATTTCGTGGGCCC ACGTGGGCCCCATCCTAGTCAGTTTGAAAATTCCCGGGGCACTCACCCTGGCCAGTTTGAAGGGGCCAGG GGCCAAGCACCTGGTTTCATGCCAGGCCCCCGGGGTATTCAGCCCCAGCAGTTCGAAGAGCAGAGAGTGA ACTCGCCACCAAGGTTTGCCGGCCAGAGGGCATCGGCGCCCCTGCCATATGGAGGACCAAGGGGCCCTGC ACCCTTTCCTGAAAAAAATGAGCAGCCACCTTCTCGATTTCACTTTCAAGGCCCATCTTCACAGCCCGTG AAGCCTCCCCCGAGGCCCCTGTTGGAGCTACCAAGCCATCCTCCTCAGCATAGGAAGGACCGATGGGATG AGGCAGGCCCAGCCACTGCTCTGCCTTCCAGTGCTGGGCCTGGACAGGGCCATGAGGCCGACGGGCAGTG GGCCACATCAGAGTTCCGAGAAGGCAAAGGCCATGAATATAGGAGCCCAGCTTTTGAAGGGAGACAGAGG GAGCGATTTGAAGCAGGATCTAAAGAAAAGCCTCTGGACGAGCCTGAAGCCCAAGGGTTGGAAAGCCGGC AGGGCCGGGCATTTGAGGATAGGAGGCGAGAGCGAGAGAGAGGCAGAAACTGGAGTCGAGAAAGAGACTG GGAGAGGAGCCGGGACTGGGACCGGCATCGGGAGTGGGATAAAGGCAGGGATAGGAGCTCCAACAGGGAC AGGGAGCGAGACAACGACAGGGCTAAGGAATGGGACCGGAGCCGCGAGAGAAGCCGGAATCGCGACCGGG ACCGAGAAAGGCGGCGAGACCGAGACAGGTCCCGCAGCAGAGACCGAGACAGGGACCGTGAGAGAGCACG AGACAGGGACAGGGACCGAGGCCGGGACCGGAAGGACCGCAGCAAGAGCAGAGAGAGCCCTCGGGACCAG AAGCCTGAGGCCAGGACCTCTGAGGGGGGCCCTGCTGCAGCCCAGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225049 representing NM_175551
Red=Cloning site Green=Tags(s) MDDKGHLSNEEAPKAIKPTSKEFRKTWGFRRTTIAKREGAGDTEVDPSEQQPQQHNLSLRRSGRQPKRTE RVEEFLTTVRRRGKKNVPVSLEDSSEPTSSTVTDVETASEGSVESSSEIRSGPVSDSLGKEHPASSEKAK GGEEEEDTSDSDSDGLTLKELQNRLRRKREQEPVERSLRGSQNRLRKKRREEDSAETGSVQIGSAEQDRP LCKQEPEASQGPVSQSETDDIENQLEGKATQGNTEENPREAGKPKPECEVYDPNALYCICRQPHNNRFMI CCDRCEEWFHGDCVGISEARGRLLERNGEDYICPNCTILQVQDETNGSATDEQDSGCRSVGADGTDCTSI GTVEQKSGEDQGIKGRIEKAANPSGKKKLKIFQPVVEAPGAPKCIGPGCSSVAQPDSVYCSNDCILKHAA ATMRFLSSGKEQKTKPKEKVKTKPEKFSLPKCSVQVGIKISSVHKRLASEKRENPVKKVMLASRSETSGK EAACESSTPSWASDHNYNAVKPEKPEKPTALSPTLLSKSMKDDRRVEDRTMAAVTIPKKALPSASLVGRQ TSPRNLVPKKLPPYSNMAGAKPAIKKLPSGFKGTIPKRPWPSATLSGTSARQAGPTPMTAASKKLPGSAA VVGVTRKPMSANVPAASPAPGRLGPVSPAPSQPNSQIRQNIRRSLKEILWKRVNDSDDLIMTENEVGKIA LHIEKEMFNLFQVTDNRYKSKYRSIMFNLKDPKNQGLFHRVLREEISLAKLVRMKPEELVSKELSMWTEK PTKSVIESRTKLLNESKKNTTKPETIPDMEDSPPVSDSEEQQESVRAAPEKSAAPLLDVFSSMLKDTTSQ HRAHLFDLNCKICTGQVPSSEDEPAPKKQKLSASSKKEDFKPRHDSSPPNAVPNTADEGIADTLPENASE PDPESTSSLNQERKCFPESPGDSHPEPSSLGGLSPSSASGGSGVVTTVTMSGRDPRTALSGSCTVTASMA AHLDNSQASETKLDMIKPALTSAVVPKSILAKPSSSPDPRYLSVPPSPSISESRSPPEGDTTLFLSRLNT IWKGFINMQSVAKFVTKAYPVSGCLDYLSEDLPDTIHIGGRIAPKTVWDYVGKLKSSVSKELCLIRFHPA TEEEEVAYISLYSYFSSRGRFGVVANNNRHVKDLYLIPLSAKDPVPSKLLPFEGPGLESPRPNIILGLVI CQKVKRPSSAGELDKTDEKRTRLQQEELETSVYPKVTAALPSEKKPPKYSVHSIDTAATSTTPPGSPPPP PPLPEPPVLKILSSLKPGSTSTVTAPTTAAITTTASPVTAATSKTASPLEHILQTLFGKKKSFEPSGKES VGSTLSPHQDSKAKGEDTMSAAPLLDPIVQQFGQFSKDKALEEEEEDDRPYDPEEEYNPDRAFHTLLAEP GRPHDVQSVSETAEREEVAYDPEDETILEEAKVTIDDLPNRMCMKVSATERPADFTTDASSASLVEQQKM LEELNKQIEEQKRQLEEQEEALRQQRAAVGVSMAHFSVSDALMSPPPKSSLGKTELFSQEQQAPDPSQGA PNTNHNLDSRQSRDPRQARRLAAENTENESLPRAPTGSTPGPQGTLPARETPAGTAVVQGPGLAAEAKES MAVPWAPGENAVLRPEHDIQKCEHPGNPVSLPLDTSHLPTAGDGAARPAPPRRVLLPTPPSTTFPPSFPL QPKAQNFSSGSREPFSGPTFMSQETSLGSSQYEDPRGAQSAGKNDSPVADMEDSREPQPRPGESTTSFPQ PGQRGGGPQPQFPGQREPAPRTFGMSGHHGPSFPGPRGPVPPYSEENLVPNSDGPRGPPPARFGAQKPPI PSLFSGQHGPPPYGDNRGLSPSYLGGPRGGAPAQFEDRKDPHGEKREFQDTPYNEMTGAPAQCEGPDQAQ FMGNRAPFQFGGQRRPLLTQMKGPRGGPPPSQFGAQRGPPPGHFVGPRGPHPSQFENSRGTHPGQFEGAR GQAPGFMPGPRGIQPQQFEEQRVNSPPRFAGQRASAPLPYGGPRGPAPFPEKNEQPPSRFHFQGPSSQPV KPPPRPLLELPSHPPQHRKDRWDEAGPATALPSSAGPGQGHEADGQWATSEFREGKGHEYRSPAFEGRQR ERFEAGSKEKPLDEPEAQGLESRQGRAFEDRRRERERGRNWSRERDWERSRDWDRHREWDKGRDRSSNRD RERDNDRAKEWDRSRERSRNRDRDRERRRDRDRSRSRDRDRDRERARDRDRDRGRDRKDRSKSRESPRDQ KPEARTSEGGPAAAQA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175551 |
| ORF Size | 6768 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175551.4, NP_780760.2 |
| RefSeq Size | 8473 bp |
| RefSeq ORF | 6771 bp |
| Locus ID | 23856 |
| UniProt ID | Q8C9B9 |
| Gene Summary | This gene encodes a transcription factor involved in apoptosis. The encoded protein functions in cell cycle progression and plays a role in chromosomal stability. This protein regulates the self-renewal of embryonic stem cells. Disruption of this gene in mice causes symptoms similar to myelodysplastic/myeloproliferative diseases in humans. Mice lacking this gene show severely reduced fertility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
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