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Eri1 (NM_026067) Mouse Tagged ORF Clone
CAT＃: MG225061
- TrueORF®
Eri1 (tGFP-tagged) - Mouse exoribonuclease 1 (Eri1), (10ug)
ORF Plasmid: DDK
"NM_026067" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3'hexo; 3110010F15Rik; eri-1; Thex1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225061 representing NM_026067
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACGAGCGGGGCCGGGAGCGTGGCGGCGACGCGGCGCAGCAGAAGACGCCGCGGCCGGAGTGTG AGGAGTCCCGGCCGCTGAGCGTGGAGAAAAAGCAGCGATGCAGACTTGATGGCAAAGAGACAGATGGATC GAAGTTCATTTCCTCCAATGGCAGTGACTTCAGTGACCCGGTTTACAAAGAGATCGCAATGACAAACGGT TGTATCAACCGGATGAGTAAGGAGGAACTCAGAGCTAAACTTTCAGAATTTAAGCTTGAAACAAGAGGAG TCAAGGATGTTCTAAAGAAGAGACTGAAAAACTATTACAAGAAGCAGAAGTTGATGCTGAAAGAGAGCTC CGCTGGGGACAGTTACTATGACTACATTTGTATCATTGACTTTGAGGCTACTTGTGAAGAGGGGAACCCA GCTGAGTTCCTACATGAAATCATTGAGTTTCCTGTTGTCCTGTTGAATACACACACCTTAGAAATCGAAG ATACGTTTCAGCAGTATGTGAGGCCAGAAGTCAATGATCAGCTTTCAGAGTTCTGCATCGGTCTTACTGG AATCACTCAGGATCAGGTAGACAGAGCCGATGCTTTCCCTCAGGTCCTGAAAAAAGTGATTGAGTGGATG AAGTCAAAGGAGTTGGGAACTAAGTACAAGTACTGTATACTAACAGATGGCTCCTGGGATATGAGTAAGT TCCTGAGCATCCAGTGCCGGCTCAGCAGACTCAAACACCCGGCCTTCGCTAAGAAGTGGATCAATATCCG GAAGTCATACGGAAACTTCTATAAGGTTCCCAGAAGCCAGACCAAACTAACAATAATGCTTGAAAAATTA GGAATGGATTATGATGGACGGCCTCACAGCGGTCTCGATGACTCTAAGAACATTGCTAGAATAGCTATTC GGATGCTTCAGGATGGATGTGAACTTCGAATCAATGAAAAAATACTTGGAGGGCAGTTGATGAGTGTGTC CTCATCCTTACCAGTAGAGGGCGCTCCAGCTCCACAGATGCCACATAGTAGAAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225061 representing NM_026067
Red=Cloning site Green=Tags(s) MEDERGRERGGDAAQQKTPRPECEESRPLSVEKKQRCRLDGKETDGSKFISSNGSDFSDPVYKEIAMTNG CINRMSKEELRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKESSAGDSYYDYICIIDFEATCEEGNP AEFLHEIIEFPVVLLNTHTLEIEDTFQQYVRPEVNDQLSEFCIGLTGITQDQVDRADAFPQVLKKVIEWM KSKELGTKYKYCILTDGSWDMSKFLSIQCRLSRLKHPAFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKL GMDYDGRPHSGLDDSKNIARIAIRMLQDGCELRINEKILGGQLMSVSSSLPVEGAPAPQMPHSRK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026067 |
| ORF Size | 1035 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026067.3, NP_080343.4 |
| RefSeq Size | 5067 bp |
| RefSeq ORF | 1038 bp |
| Locus ID | 67276 |
| UniProt ID | Q7TMF2 |
| Gene Summary | RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Binds with high affinity to the 3' side of the stem-loop structure and to the downstream cleavage product (DCP) of histone pre-mRNAs. Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs (By similarity). Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210515 | Eri1 (untagged) - Mouse exoribonuclease 1 (Eri1), (10ug) |
CNY 3,656.00 |
|
| MR225061 | Eri1 (Myc-DDK-tagged) - Mouse exoribonuclease 1 (Eri1) |
CNY 3,656.00 |
|
| MR225061L3 | Lenti ORF clone of Eri1 (Myc-DDK-tagged) - Mouse exoribonuclease 1 (Eri1) |
CNY 5,230.00 |
|
| MR225061L4 | Lenti ORF clone of Eri1 (mGFP-tagged) - Mouse exoribonuclease 1 (Eri1) |
CNY 5,230.00 |
