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Tnfaip1 (NM_009395) Mouse Tagged ORF Clone
CAT＃: MG225245
- TrueORF®
Tnfaip1 (tGFP-tagged) - Mouse tumor necrosis factor alpha-induced protein 1 (endothelial) (Tnfaip1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_009395" in other vectors (5)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Bacurd2; Edp-1; Edp1; Tnfip1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225245 representing NM_009395
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGGGGACACCTGTCTGTGTCCAGCCTCGGGGGCCAAGCCCAAGATCAGTGGCTTCAAGGGAGGCG GGCTGGGCAACAAGTACGTGCAGCTCAATGTGGGAGGCTCCCTGTACTACACCACAGTGCGGGCCCTCAC CCGGCACGACACCATGCTCAAGGCCATGTTCAGTGGGCGAATGGAGGTGCTGACTGACAAAGAAGGCTGG ATTCTCATAGACCGATGTGGAAAGCACTTTGGCACCATCTTGAATTACCTCCGAGATGACACCATCACCC TCCCTCAAAGCCGGCAAGAAATCCAGGAGTTGATGGCTGAAGCAAAATATTACCTCATTCAAGGGCTGGT GAGCACGTGCCAGACTGCCCTGCAGGACAAGAAAGACTCCTACCAGCCTGTGTGCAACATCCCCATCATC ACGTCCCTGAGGGAGGAGGACAGGCTCATTGAATCCTCCACAAAGCCTGTGGTGAAGCTGCTGTACAACA GGAGCAACAACAAGTACTCATACACCAGCAACTCTGATGATCACCTGCTAAAGAACATTGAACTGTTTGA CAAGCTGTCCCTGCGCTTCAACGGCCGCGTGCTCTTCATCAAGGATGTCATTGGGGACGAGATCTGCTGC TGGTCTTTCTATGGCCAGGGCCGTAAGCTGGCAGAGGTGTGCTGCACCTCGATTGTGTACGCCACAGAGA AGAAGCAGACCAAGGTGGAATTCCCAGAGGCCCGTATCTATGAGGAAACACTCAATGTCCTACTCTATGA GACCCCTCGAGTCCCTGACAATTCCTTACTGGAGGCCACAAGCCGGAGCCGCAGCCAGGCTTCCCCCAGT GAAGATGAAGACACCTTTGAACTACGGGACCGTGTCCGTCGCATTCATGTCAAGCGCTATAGCACTTACG ATGACCGGCAACTTGGCCATCAGTCCACTCATCGTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225245 representing NM_009395
Red=Cloning site Green=Tags(s) MSGDTCLCPASGAKPKISGFKGGGLGNKYVQLNVGGSLYYTTVRALTRHDTMLKAMFSGRMEVLTDKEGW ILIDRCGKHFGTILNYLRDDTITLPQSRQEIQELMAEAKYYLIQGLVSTCQTALQDKKDSYQPVCNIPII TSLREEDRLIESSTKPVVKLLYNRSNNKYSYTSNSDDHLLKNIELFDKLSLRFNGRVLFIKDVIGDEICC WSFYGQGRKLAEVCCTSIVYATEKKQTKVEFPEARIYEETLNVLLYETPRVPDNSLLEATSRSRSQASPS EDEDTFELRDRVRRIHVKRYSTYDDRQLGHQSTHRD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009395 |
| ORF Size | 948 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009395.4, NP_033421.3 |
| RefSeq Size | 3709 bp |
| RefSeq ORF | 951 bp |
| Locus ID | 21927 |
| UniProt ID | O70479 |
| Gene Summary | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(TNFAIP1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205667 | Tnfaip1 (untagged) - Mouse tumor necrosis factor, alpha-induced protein 1 (endothelial) (Tnfaip1), transcript variant 1, (10ug) |
CNY 2,400.00 |
|
| MG204517 | Tnfaip1 (tGFP-tagged) - Mouse tumor necrosis factor, alpha-induced protein 1 (endothelial) (Tnfaip1) |
CNY 2,850.00 |
|
| MR225245 | Tnfaip1 (Myc-DDK-tagged) - Mouse tumor necrosis factor, alpha-induced protein 1 (endothelial) (Tnfaip1), transcript variant 1 |
CNY 2,400.00 |
|
| MR225245L3 | Lenti ORF clone of Tnfaip1 (Myc-DDK-tagged) - Mouse tumor necrosis factor, alpha-induced protein 1 (endothelial) (Tnfaip1), transcript variant 1 |
CNY 4,750.00 |
|
| MR225245L4 | Lenti ORF clone of Tnfaip1 (mGFP-tagged) - Mouse tumor necrosis factor, alpha-induced protein 1 (endothelial) (Tnfaip1), transcript variant 1 |
CNY 4,750.00 |
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