United States
Germany
Japan
United Kingdom
China
Cst3 (NM_009976) Mouse Tagged ORF Clone
CAT＃: MG225378
- TrueORF®
Cst3 (tGFP-tagged) - Mouse cystatin C (Cst3), (10ug)
ORF Plasmid: DDK
"NM_009976" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Cys; CysC |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225378 representing NM_009976
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCCCGCTGCGCTCCTTGCTGTTCCTGCTGGCCGTCCTGGCCGTGGCCTGGGCGGCGACCCCAA AACAAGGCCCGCGAATGTTGGGAGCCCCGGAGGAGGCAGATGCGAATGAGGAAGGCGTGCGGCGAGCGTT GGACTTCGCTGTGAGCGAGTACAACAAGGGCAGCAACGATGCGTACCACAGCCGCGCCATACAGGTGGTG AGAGCTCGTAAGCAGCTCGTGGCTGGAGTGAACTATTTTTTGGATGTGGAGATGGGCCGAACTACATGTA CCAAGTCCCAGACAAATTTGACTGACTGTCCTTTCCATGACCAGCCCCATCTGATGAGGAAGGCACTCTG CTCCTTCCAGATCTACAGCGTGCCCTGGAAAGGCACACACTCCCTGACAAAATTCAGCTGCAAAAATGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225378 representing NM_009976
Red=Cloning site Green=Tags(s) MASPLRSLLFLLAVLAVAWAATPKQGPRMLGAPEEADANEEGVRRALDFAVSEYNKGSNDAYHSRAIQVV RARKQLVAGVNYFLDVEMGRTTCTKSQTNLTDCPFHDQPHLMRKALCSFQIYSVPWKGTHSLTKFSCKNA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009976 |
| ORF Size | 420 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009976.3, NP_034106.2 |
| RefSeq Size | 748 bp |
| RefSeq ORF | 423 bp |
| Locus ID | 13010 |
| UniProt ID | P21460 |
| Gene Summary | The protein encoded by this gene is a cysteine protease inhibitor involved in neurodegenerative and cardiovascular processes. The encoded protein inhibits aggregation of beta-amyloid protein, a hallmark of Alzheimer's disease, so it may be useful as a therapeutic. This protein also may be a biomarker for atherosclerosis. [provided by RefSeq, Aug 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208349 | Cst3 (untagged) - Mouse cystatin C (Cst3), (10ug) |
CNY 3,990.00 |
|
| MR225378 | Cst3 (Myc-DDK-tagged) - Mouse cystatin C (Cst3) |
CNY 1,200.00 |
|
| MR225378L3 | Lenti ORF clone of Cst3 (Myc-DDK-tagged) - Mouse cystatin C (Cst3) |
CNY 4,750.00 |
|
| MR225378L4 | Lenti ORF clone of Cst3 (mGFP-tagged) - Mouse cystatin C (Cst3) |
CNY 4,750.00 |
