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Hcn4 (NM_001081192) Mouse Tagged ORF Clone
CAT＃: MG225484
- TrueORF®
Hcn4 (tGFP-tagged) - Mouse hyperpolarization-activated cyclic nucleotide-gated K+ 4 (Hcn4), (10ug)
ORF Plasmid: DDK
"NM_001081192" in other vectors (4)
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CNY 14,144.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Bcng3; Hcn3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225484 representing NM_001081192
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAAGCTGCCGCCGTCCATGCGCAAGCGGCTCTACAGCCTTCCGCAGCAGGTGGGGGCCAAGGCGT GGATCATGGACGAGGAAGAGGATGGTGAGGAAGAAGGGGCCGGGGGCCGCCAGGACCCCAGCCGAAGGAG CATCCGGCTGCGGCCGCTGCCCTCGCCCTCTCCCTCGGTGGCTGCGGGCTGCTCGGAGTCCCGGGGTGCG GCCCTCGGGGCGACAGAGAGCGAGGGGCCGGGCCGCAGCGCCGGCAAGTCCAGCACCAACGGTGACTGCA GGCGCTTCCGCGGGAGTCTGGCCTCGCTGGGCAGCCGGGGCGGCGGCAGTGGTGGAGCAGGGGGCGGCAG CAGTCTCGGGCACCTGCATGACTCCGCGGAGGAACGGCGGCTCATCGCCGCTGAGGGCGATGCGTCCCCC GGCGAGGACAGGACGCCCCCGGGCCTGGCGACCGAACCCGAGCGCCCGGCCACCGCGGCACAACCCGCAG CCTCGCCGCCGCCCCAGCAGCCGCCGCAGCCGGCCTCTGCCTCCTGCGAGCAGCCCTCGGCGGACACCGC TATCAAAGTGGAGGGAGGCGCGGCCGCCAGCGACCAGATCCTCCCCGAGGCCGAGGTGCGCCTGGGCCAG AGCGGCTTCATGCAGCGCCAGTTCGGTGCCATGCTGCAACCTGGGGTCAACAAATTCTCCCTAAGGATGT TCGGCAGCCAGAAAGCGGTGGAGCGCGAGCAGGAGAGGGTTAAGTCAGCAGGGTTTTGGATTATCCACCC CTACAGTGACTTCAGATTTTACTGGGACCTGACGATGCTGTTGCTGATGGTGGGGAATCTGATCATCATA CCCGTGGGCATCACCTTCTTCAAGGATGAGAACACCACACCCTGGATCGTCTTCAATGTGGTGTCAGACA CATTCTTCCTCATTGACTTGGTCCTCAACTTCCGCACGGGGATCGTGGTGGAGGACAACACAGAAATCAT CCTTGACCCGCAGAGGATCAAGATGAAGTACCTGAAAAGCTGGTTTGTGGTAGATTTCATCTCCTCCATC CCTGTCGACTACATCTTCCTTATAGTGGAGACTCGCATTGACTCGGAGGTCTACAAAACCGCTAGGGCTC TGCGCATTGTCCGTTTCACTAAGATCCTCAGCCTCCTGCGCCTCTTGAGGCTTTCCCGCCTCATTCGATA CATTCATCAGTGGGAAGAGATCTTCCACATGACCTATGACCTGGCCAGCGCCGTGGTACGCATCGTGAAC CTCATTGGCATGATGCTTCTGCTGTGTCACTGGGATGGCTGCCTGCAGTTCCTAGTGCCCATGCTGCAGG ACTTCCCCCATGACTGCTGGGTGTCCATCAATGGCATGGTGAATAACTCCTGGGGGAAGCAGTATTCCTA CGCCCTCTTCAAGGCCATGAGCCACATGCTGTGCATTGGGTATGGACGGCAGGCACCCGTAGGCATGTCT GACGTCTGGCTCACCATGCTCAGCATGATCGTGGGGGCCACCTGCTATGCCATGTTCATCGGCCACGCCA CTGCCCTCATCCAGTCGCTAGACTCCTCCCGGCGCCAGTACCAGGAGAAGTATAAACAGGTGGAGCAGTA CATGTCCTTCCACAAGCTCCCGCCTGACACCCGACAGCGCATCCATGACTACTATGAACACCGCTACCAA GGCAAGATGTTTGATGAGGAAAGCATCCTGGGTGAGCTGAGTGAGCCACTTCGAGAGGAGATCATCAACT TTAACTGCCGAAAGCTGGTGGCATCCATGCCACTGTTTGCCAACGCAGATCCCAACTTTGTGACATCCAT GCTGACCAAGTTGCGTTTCGAGGTCTTCCAGCCTGGGGATTACATCATCCGCGAAGGCACCATCGGCAAG AAGATGTACTTTATCCAGCACGGCGTGGTCAGCGTGCTCACTAAGGGCAACAAAGAGACCAAGCTGGCTG ATGGCTCCTATTTTGGAGAGATCTGCTTGCTGACCCGGGGTCGGCGCACAGCCAGCGTCAGAGCGGATAC TTATTGCCGCCTCTACTCACTGAGCGTGGACAACTTCAATGAGGTGCTGGAGGAGTATCCCATGATGCGG AGGGCCTTCGAGACGGTTGCGCTGGACCGCCTGGACCGCATAGGCAAGAAGAACTCCATCCTCCTCCACA AGGTGCAGCACGACCTCAACTCAGGCGTCTTCAACTACCAAGAGAACGAGATCATCCAGCAGATCGTGCG GCATGACCGTGAGATGGCCCACTGTGCTCACCGCGTCCAGGCTGCCGCCTCAGCCACCCCAACCCCCACG CCTGTTATATGGACCCCGCTGATCCAGGCGCCACTGCAGGCTGCTGCTGCTACTACTTCGGTGGCCATAG CCCTCACACACCACCCCCGCCTGCCCGCCGCCATCTTCCGGCCCCCTCCCGGACCTGGGCTGGGCAACCT TGGGGCTGGACAGACACCGAGGCACCCAAGGAGGCTGCAGTCCTTGATCCCTTCAGCTCTGGGCTCTGCT TCACCCGCCAGCAGCCCCTCACAGGTGGACACACCGTCTTCATCCTCCTTCCACATCCAACAGCTGGCTG GATTCTCTGCACCTCCTGGATTGAGCCCTCTCCTGCCCTCCTCTAGCTCTTCCCCACCTCCAGGAGCCTG CGGTTCCCCACCAGCCCCCACACCCTCCACCTCCACTGCCGCCGCCGCCTCCACCACTGGGTTCGGCCAC TTTCACAAGGCGCTGGGTGGCTCCCTGTCATCCTCTGACTCCCCGCTGCTCACCCCACTGCAACCAGGCG CTCGCTCTCCACAGGCTGCCCAGCCACCACCCCCACTGCCTGGGGCCCGAGGAGGTCTGGGACTCCTGGA GCACTTCTTGCCGCCCCCACCCTCCTCCAGGTCACCATCATCCAGCCCTGGGCAGCTGGGCCAGCCTCCT GGAGAGTTGTCCCTAGGTCTGGCAGCTGGTCCATCAAGTACACCAGAGACACCCCCACGGCCTGAGCGAC CATCCTTCATGGCAGGGGCCTCTGGAGGGGCTTCTCCTGTAGCCTTTACCCCCCGAGGAGGCCTCAGTCC TCCGGGCCACAGCCCGGGGCCCCCAAGAACTTTCCCGAGTGCCCCACCCCGGGCCTCTGGCTCCCATGGT TCCCTGCTCCTGCCACCTGCATCCAGCCCTCCACCTCCCCAGGTCCCACAGCGCAGGGGCACACCACCCC TCACCCCTGGCCGCCTCACACAGGACCTGAAGCTCATCTCAGCCTCTCAGCCAGCCCTCCCCCAGGATGG GGCACAGACTCTCCGCAGGGCCTCGCCTCACTCCTCAGGGGAGTCGGTGGCTGCCTTCTCACTCTACCCC AGAGCTGGGGGTGGCAGTGGGAGTAGTGGGGGCCTTGGGCCTCCTGGAAGGCCATATGGTGCCATCCCAG GCCAACATGTCACTTTGCCTCGGAAGACATCCTCAGGTTCTTTGCCACCCCCACTTTCTTTGTTTGGGGC AAGAGCCGCCTCTTCTGGAGGGCCCCCTCTGACTACTGCTGCACCCCAGAGGGAACCTGGCGCTAGGTCT GAGCCAGTACGCTCCAAACTGCCGTCTAATTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225484 representing NM_001081192
Red=Cloning site Green=Tags(s) MDKLPPSMRKRLYSLPQQVGAKAWIMDEEEDGEEEGAGGRQDPSRRSIRLRPLPSPSPSVAAGCSESRGA ALGATESEGPGRSAGKSSTNGDCRRFRGSLASLGSRGGGSGGAGGGSSLGHLHDSAEERRLIAAEGDASP GEDRTPPGLATEPERPATAAQPAASPPPQQPPQPASASCEQPSADTAIKVEGGAAASDQILPEAEVRLGQ SGFMQRQFGAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIII PVGITFFKDENTTPWIVFNVVSDTFFLIDLVLNFRTGIVVEDNTEIILDPQRIKMKYLKSWFVVDFISSI PVDYIFLIVETRIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIVN LIGMMLLLCHWDGCLQFLVPMLQDFPHDCWVSINGMVNNSWGKQYSYALFKAMSHMLCIGYGRQAPVGMS DVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPPDTRQRIHDYYEHRYQ GKMFDEESILGELSEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIREGTIGK KMYFIQHGVVSVLTKGNKETKLADGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMR RAFETVALDRLDRIGKKNSILLHKVQHDLNSGVFNYQENEIIQQIVRHDREMAHCAHRVQAAASATPTPT PVIWTPLIQAPLQAAAATTSVAIALTHHPRLPAAIFRPPPGPGLGNLGAGQTPRHPRRLQSLIPSALGSA SPASSPSQVDTPSSSSFHIQQLAGFSAPPGLSPLLPSSSSSPPPGACGSPPAPTPSTSTAAAASTTGFGH FHKALGGSLSSSDSPLLTPLQPGARSPQAAQPPPPLPGARGGLGLLEHFLPPPPSSRSPSSSPGQLGQPP GELSLGLAAGPSSTPETPPRPERPSFMAGASGGASPVAFTPRGGLSPPGHSPGPPRTFPSAPPRASGSHG SLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKLISASQPALPQDGAQTLRRASPHSSGESVAAFSLYP RAGGGSGSSGGLGPPGRPYGAIPGQHVTLPRKTSSGSLPPPLSLFGARAASSGGPPLTTAAPQREPGARS EPVRSKLPSNL TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081192 |
| ORF Size | 3603 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081192.3 |
| RefSeq Size | 3798 bp |
| RefSeq ORF | 3606 bp |
| Locus ID | 330953 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223934 | Hcn4 (untagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4), (10ug) |
CNY 15,390.00 |
|
| MR225484 | Hcn4 (Myc-DDK-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4) |
CNY 12,544.00 |
|
| MR225484L3 | Lenti ORF clone of Hcn4 (Myc-DDK-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4) |
CNY 13,870.00 |
|
| MR225484L4 | Lenti ORF clone of Hcn4 (mGFP-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4) |
CNY 13,870.00 |
