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Pias2 (NM_001164169) Mouse Tagged ORF Clone
CAT＃: MG225573
- TrueORF®
Pias2 (tGFP-tagged) - Mouse protein inhibitor of activated STAT 2 (Pias2) transcript variant 4, (10ug)
ORF Plasmid: DDK
"NM_001164169" in other vectors (3)
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CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6330408K17Rik; AI462206; ARIP3; AU018068; Dib; Miz1; PIASxalpha; PIASxb; PIASxbeta; SIZ2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225573 representing NM_001164169
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATTTCGAGGAGTTGAGGAATATGGTTTCTAGTTTTAGGGTTTCTGAGTTACAAGTGTTACTGG GCTTTGCTGGACGGAATAAAAGTGGGCGCAAGCATGACCTCCTGATGAGGGCGTTGCATTTATTGAAGAG TGGCTGCAGCCCTGCGGTTCAGATTAAAATTCGAGAATTATATAGACGCCGATACCCACGGACACTTGAA GGACTTTGTGATCTTTCCACAATCAAATCATCGGTTTTCAGCTTGGATGGTAGCTCATCACCCGTAGAAC CTGACTTGCCTGTGGCTGGGATCCACTCGTTGCCTTCGACTTCAATTACACCTCATTCACCGTCATCTCC TGTTGGTTCTGTACTGCTGCAAGACACTAAGCCCACCTTTGAGATGCAGCAGCCGTCTCCGCCCATTCCT CCTGTCCATCCTGATGTGCAGTTAAAAAACCTGCCTTTCTATGATGTCCTTGATGTTCTCATCAAGCCCA CGAGTTTAGTTCAAAGCAGTATTCAGCGGTTTCAAGAGAAGTTTTTTATTTTTGCTTTGACACCTCAGCA AGTTAGAGAAATATGTATTTCAAGGGACTTTTTGCCGGGTGGCAGGAGAGACTACACAGTCCAAGTCCAG CTGCGACTTTGCTTGGCAGAGACCAGTTGCCCTCAAGAAGATAACTATCCCAATAGTTTGTGTATAAAAG TCAATGGGAAACTCTTTCCTTTGCCTGGCTATGCGCCACCACCTAAAAATGGGATTGAACAGAAGCGCCC TGGACGCCCCCTGAACATTACGTCTTTAGTTAGGTTGTCTTCAGCTGTGCCAAATCAGATTTCTATTTCT TGGGCATCTGAAATTGGAAAGAATTACTCCATGTCTGTGTATCTTGTACGACAGCTTACATCAGCCATGT TATTACAGAGATTAAAAATGAAAGGTATTAGAAATCCTGATCATTCCAGAGCACTAATTAAAGAAAAACT TACTGCAGACCCTGATAGTGAAATTGCTACAACTAGTCTTCGAGTGTCCTTGATGTGCCCTTTAGGAAAA ATGAGGCTGACAATCCCGTGCCGTGCAGTGACTTGTACACATCTGCAGTGCTTTGATGCTGCCCTCTATC TTCAGATGAATGAGAAAAAGCCCACCTGGATCTGTCCTGTTTGTGACAAAAAGGCTGCCTATGAGAGTCT GATACTAGATGGGCTTTTTATGGAAATTCTCAATGACTGTTCTGATGTGGATGAGATCAAATTCCAGGAA GATGGTTCTTGGTGTCCCATGAGACCTAAAAAAGAAGCTATGAAAGTAACCAGCCAGCCCTGTACAAAAG TAGAAAGTTCAAGTGTCTTTAGTAAACCTTGTTCAGTGACTGTAGCCAGTGATGCAAGCAAGAAGAAGAT AGATGTTATTGATCTAACAATAGAGAGCTCTTCTGATGAAGAGGAAGACCCTCCCGCCAAAAGGAAATGC ATCTTTATGTCAGAAACACAAAGCAGTCCAACCAAAGGGGTTCTCATGTATCAGCCATCTTCTGTAAGGG TGCCCAGTGTGACTTCAGTTGATCCTGCTGCTATTCCACCTTCATTAACAGACTACTCAGTACCATTCCA CCACACGCCAGTGTCAAGCATGTCATCAGATTTGCCAGGAGAACAAAGAAGAAATGATATTAATAACGAA GTGCAGCTTGGAGCATCTTCTGATACTGTGCAACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225573 representing NM_001164169
Red=Cloning site Green=Tags(s) MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIRELYRRRYPRTLE GLCDLSTIKSSVFSLDGSSSPVEPDLPVAGIHSLPSTSITPHSPSSPVGSVLLQDTKPTFEMQQPSPPIP PVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQ LRLCLAETSCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISIS WASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGK MRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQE DGSWCPMRPKKEAMKVTSQPCTKVESSSVFSKPCSVTVASDASKKKIDVIDLTIESSSDEEEDPPAKRKC IFMSETQSSPTKGVLMYQPSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTPVSSMSSDLPGEQRRNDINNE VQLGASSDTVQQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001164169 |
| ORF Size | 1716 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164169.1, NP_001157641.1 |
| RefSeq Size | 2073 bp |
| RefSeq ORF | 1719 bp |
| Locus ID | 17344 |
| UniProt ID | Q8C5D8 |
| Gene Summary | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIASx-beta, but not isoform PIASx-alpha, promotes MDM2 sumoylation. Isoform PIASx-alpha promotes PARK7 sumoylation. Isoform PIASx-beta promotes NCOA2 sumoylation more efficiently than isoform PIASx-alpha (By similarity). Sumoylates PML at'Lys-65' and 'Lys-160' (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR225573 | Pias2 (Myc-DDK-tagged) - Mouse protein inhibitor of activated STAT 2 (Pias2), transcript variant 4 |
CNY 5,700.00 |
|
| MR225573L3 | Lenti ORF clone of Pias2 (Myc-DDK-tagged) - Mouse protein inhibitor of activated STAT 2 (Pias2), transcript variant 4 |
CNY 7,600.00 |
|
| MR225573L4 | Lenti ORF clone of Pias2 (mGFP-tagged) - Mouse protein inhibitor of activated STAT 2 (Pias2), transcript variant 4 |
CNY 7,600.00 |
