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Shc1 (NM_001113331) Mouse Tagged ORF Clone
CAT＃: MG225873
- TrueORF®
Shc1 (tGFP-tagged) - Mouse src homology 2 domain-containing transforming protein C1 (Shc1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001113331" in other vectors (4)
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CNY 8,496.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | p66; p66shc; Shc; ShcA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225873 representing NM_001113331
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTTCTACCCCCCAAGCCGAAGTACAACCCACTTCGGAATGAGTCTCTGTCATCGCTGGAGGAGG GGGCTTCGGGGTCTACCCCTCCGGAGGAGCTACCTTCCCCATCAGCTTCATCCCTGGGACCCATTCTGCC TCCTCTGCCGGGGGACGATAGTCCGACTACCCTGTGTTCCTTCTTTCCCCGGATGAGCAACCTGAAGCTG GCCAATCCTGCTGGGGGGCGCCTGGGGCCTAAAGGGGAGCCAGGAAAGGCTGCTGAAGATGGGGAAGGGA GTGCAGGGGCAGCCCTTCGGGACTCAGGCCTCTTGCCCCTCCTCCAGGACATGAACAAGCTGAGTGGAGG CGGCGGGCGCAGGACTCGGGTAGAAGGGGGCCAGCTGGGGGGCGAGGAGTGGACCAGACACGGGAGCTTT GTCAATAAGCCCACACGAGGCTGGCTGCATCCCAACGACAAAGTCATGGGACCTGGGGTTTCCTACTTGG TTCGGTACATGGGCTGTGTGGAGGTCTTACAGTCAATGCGAGCCCTTGACTTCAATACCCGGACTCAGGT CACCAGGGAGGCCATCAGTTTGGTGTGTGAAGCTGTGCCTGGTGCCAAAGGGGCGACAAGGAGGAGAAAG CCTTGTAGCCGCCCACTCAGCTCCATCCTGGGGAGGAGTAACCTGAAGTTTGCTGGAATGCCAATCACTC TCACTGTGTCTACCAGCAGCCTTAACCTCATGGCAGCCGACTGCAAACAGATCATTGCCAACCATCACAT GCAATCTATCTCTTTCGCGTCCGGTGGGGATCCGGACACAGCTGAGTATGTTGCCTATGTTGCCAAAGAC CCTGTGAATCAGAGAGCCTGCCATATCCTGGAGTGTCCTGAAGGGCTTGCTCAGGATGTCATCAGCACCA TCGGGCAGGCCTTTGAGTTGCGCTTCAAACAGTATCTCAGGAATCCACCGAAGCTGGTCACCCCCCATGA CAGGATGGCTGGCTTTGATGGCTCAGCTTGGGATGAGGAGGAAGAAGAGCCCCCTGACCATCAGTACTAC AATGACTTTCCAGGGAAGGAACCCCCTCTTGGTGGGGTGGTAGATATGAGGCTTCGGGAAGGGGCTGCTC GACCCACTCTGCCTAGTGCCCAGATGTCCAGCCACTTGGGAGCTACACTGCCTATAGGGCAGCATGCTGC AGGAGACCATGAAGTCCGTAAACAGATGTTGCCTCCGCCGCCTTGCCCAGGCAGAGAACTCTTCGATGAC CCCTCCTATGTCAACATCCAGAATCTAGACAAGGCCCGGCAGGCTGGGGGTGGGGCTGGGCCCCCAAATC CTTCTCTTAATGGCAGTGCACCCCGAGACCTTTTTGACATGAAGCCCTTTGAAGATGCACTTCGGGTGCC ACCCCCACCGCAGTCCATGTCCATGGCTGAGCAGCTGCAAGGGGAGCCCTGGTTCCACGGGAAGCTGAGC CGGAGGGAGGCCGAGGCGCTGCTGCAGCTCAATGGTGACTTCTTGGTGCGAGAGAGCACGACCACGCCTG GCCAGTATGTGCTCACTGGCCTGCAGAGTGGGCAGCCCAAGCACTTGCTGCTGGTGGACCCTGAAGGTGT GGTTCGGACAAAGGATCACCGCTTTGAGAGTGTCAGTCACCTGATCAGCTACCACATGGACAATCACTTG CCCATCATCTCTGCGGGCAGCGAACTGTGCCTACAGCAACCCGTGGATCGGAAAGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225873 representing NM_001113331
Red=Cloning site Green=Tags(s) MDLLPPKPKYNPLRNESLSSLEEGASGSTPPEELPSPSASSLGPILPPLPGDDSPTTLCSFFPRMSNLKL ANPAGGRLGPKGEPGKAAEDGEGSAGAALRDSGLLPLLQDMNKLSGGGGRRTRVEGGQLGGEEWTRHGSF VNKPTRGWLHPNDKVMGPGVSYLVRYMGCVEVLQSMRALDFNTRTQVTREAISLVCEAVPGAKGATRRRK PCSRPLSSILGRSNLKFAGMPITLTVSTSSLNLMAADCKQIIANHHMQSISFASGGDPDTAEYVAYVAKD PVNQRACHILECPEGLAQDVISTIGQAFELRFKQYLRNPPKLVTPHDRMAGFDGSAWDEEEEEPPDHQYY NDFPGKEPPLGGVVDMRLREGAARPTLPSAQMSSHLGATLPIGQHAAGDHEVRKQMLPPPPCPGRELFDD PSYVNIQNLDKARQAGGGAGPPNPSLNGSAPRDLFDMKPFEDALRVPPPPQSMSMAEQLQGEPWFHGKLS RREAEALLQLNGDFLVRESTTTPGQYVLTGLQSGQPKHLLLVDPEGVVRTKDHRFESVSHLISYHMDNHL PIISAGSELCLQQPVDRKV TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001113331 |
| ORF Size | 1737 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001113331.2, NP_001106802.1 |
| RefSeq Size | 3569 bp |
| RefSeq ORF | 1740 bp |
| Locus ID | 20416 |
| UniProt ID | P98083 |
| Gene Summary | Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis (By similarity). Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p47Shc and isoform p52Shc, once phosphorylated, couple activated receptor kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p47Shc and isoform p52 may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219281 | Shc1 (untagged) - Mouse src homology 2 domain-containing transforming protein C1 (Shc1), transcript variant 1, (10ug) |
CNY 7,410.00 |
|
| MR225873 | Shc1 (Myc-DDK-tagged) - Mouse src homology 2 domain-containing transforming protein C1 (Shc1), transcript variant 1 |
CNY 5,800.00 |
|
| MR225873L3 | Lenti ORF clone of Shc1 (Myc-DDK-tagged) - Mouse src homology 2 domain-containing transforming protein C1 (Shc1), transcript variant 1 |
CNY 7,700.00 |
|
| MR225873L4 | Lenti ORF clone of Shc1 (mGFP-tagged) - Mouse src homology 2 domain-containing transforming protein C1 (Shc1), transcript variant 1 |
CNY 7,700.00 |
