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Arfgef1 (NM_001102430) Mouse Tagged ORF Clone
CAT＃: MG225967
- TrueORF®
Arfgef1 (tGFP-tagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1), (10ug)
ORF Plasmid: DDK
"NM_001102430" in other vectors (2)
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CNY 22,230.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARFGEP1; BIG1; D130059B05Rik; D730028O18Rik; P200 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225967 representing NM_001102430
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGAGGGCAAGAAGACGAAGAACATGTTCCTGACCCGGGCCCTGGAGAAGATTCTGGCCGACAAGG AAGTGAAGAAGGCGCATCACTCCCAGCTGCGCAAAGCGTGCGAGGTGGCCCTAGAGGAAATAAAAGTTGA AACTGAGAAACAAAGCCCTCCTCATGGAGAAGCAAAAGCTGGATCAGGCACCCTTCCACCAGTGAAATCA AAGACAAATTTTATTGAAGCAGACAAGTACTTTTTACCCTTTGAGTTGGCATGCCAGTCCAAATGTCCCC GCATAGTTAGTACATCTCTAGATTGTTTGCAGAAACTTATTGCTTATGGGCACTTGACTGGCAGGGCTCC AGATAGTACGACACCAGGCAAAAAATTAATTGATAGAATTATTGAAACAATATGTGGCTGCTTCCAAGGT CCTCAAACAGATGAAGGAGTTCAGTTGCAGATAATAAAGGCTTTACTTACCGCAGTGACATCACAACACA TAGAAATCCATGAAGGAACTGTGCTGCAAGCTGTGAGGACATGCTACAATATCTATTTAGCCAGCAAAAA TCTCATCAATCAGACAACAGCCAAAGCTACTCTTACTCAAATGCTGAACGTTATCTTTGCACGCATGGAA AACCAAGCATTACAGGAGGCCAAACAAATGGAAAGAGAAAGACATCGGCAGCAGCAGCATTTGTTACAGT CTCCAGTAAGCCATCATGAGCCCGAGTCACCTCATCTTAGATATTTGCCACCTCAGACTGTTGATCATAT AAACCAAGAGCATGAAGGGGATCTTGAGCCCCAAACACATGATGTGGATAAAAGTCTCCAAGATGATACA GAGCCTGAAAACGGATCTGATATTTCCAGTGCAGAAAATGAACAGACAGAAGCTGATCAGGCAACTGCAG CTGAAACATTATCTAAAAATGATATCTTGTATGATGGAGACTATGAAGAAAAGCCACTTGATATTGTCCA GAGCATTGTAGAAGAAATGGTGAACATCATTGTTGGAGACATGGGAGAAGGGATGGCTATCAGTGCAAGT ACAGAAGGCAACACTGGAACTGTAGAAGATGGTAGTGACAGTGAAAACATCCAAGCAAATGGAATCCCAG GAACACCCATTTCTGTTGCATATACACCATCGTTACCTGACGACAGGTTATCTGTCTCCTCCAATGATAC TCAGGAATCTGGAAATTCTTCAGGACCTTCACCTGGTGCTAAGTTTTCCCACATTTTACAAAAGGATGCC TTTCTAGTGTTCAGGTCATTGTGTAAGCTGTCAATGAAACCACTGTCAGATGGACCACCAGATCCAAAGT CTCATGAGTTACGGTCCAAGATTCTTTCATTGCAGTTGCTTCTGTCCATTCTGCAGAATGCAGGACCTGT TTTCAGGACAAATGAGATGTTTATTAATGCTATCAAGCAGTATCTATGTGTTGCACTCTCAAAAAACGGA GTCTCATCTGTTCCAGAGGTTTTTGAGCTTTCTCTTTCCATATTTCTTACTTTGTTGTCAAACTTCAAGA CACATCTGAAGATGCAAATTGAGGTATTCTTTAAAGAAATCTTCCTGTACATTTTGGAAACGTCTACAAG CTCATTTGACCATAAGTGGATGGTTATTCAGACACTGACAAGGATCTGTGCAGATGCTCAGAGTGTGGTG GACATTTATGTAAATTATGACTGTGACCTAAATGCAGCAAATATATTTGAAAGACTAGTAAATGACCTGT CGAAAATTGCCCAAGGAAGGGGCAGCCAAGAACTTGGTATGAGTAATGTTCAGGAATTGAGCCTGCGAAA AAAGGGGTTAGAATGTTTAGTTTCAATTTTGAAATGTATGGTTGAATGGAGCAAGGATCAGTATGTGAAC CCCAACTCTCAAACTACCCTTGGTCAGGAAAAACCCTCAGAGCAAGAGATAAGTGAAGTTAAACACCCAG AGACTATAAACAGATATGGAAGTTTAAACTCCTTAGAATCCACATCATCATCAGGAATTGGCAGCTACAG TACACAGATGTCTGGCACTGATAATCCAGAACAATTTGAGGTCCTAAAGCAGCAAAAAGAAATAATAGAA CAAGGGATTGATTTATTTAATAAGAAACCAAAGAGAGGAATACAATACCTCCAAGAACAAGGGATGCTTG GCACCACACCCGAAGATATAGCTCAGTTCTTACATCAAGAAGAAAGATTAGACTCTACTCAGGTGGGTGA GTTCCTGGGAGATAATGATAAATTCAATAAAGAAGTCATGTATGCATATGTAGACCAACATGACTTTTCA GGAAAAGACTTTGTTTCAGCTCTTCGTCTATTTCTGGAAGGATTCCGGCTTCCAGGAGAAGCTCAGAAAA TTGATCGATTAATGGAAAAATTTGCTGCAAGATACCTAGAGTGCAACCAAGGACAAACCCTTTTTGCTAG TGCAGATACTGCTTATGTTTTGGCCTACTCAATTATCATGTTGACTACAGACCTTCACAGTCCACAGGTT AAAAACAAAATGACAAAAGAACAGTACATTAAAATGAATCGTGGTATCAATGACAGTAAAGATCTCCCTG AAGAGTATCTATCAGCCATTTATAACGAAATAGCTGGGAAAAAAATATCAATGAAAGAAACAAAAGAACT AACAATCCCTACAAAGTCAACTAAACAGAATGTAGCCAGTGAAAAGCAAAGACGACTTCTGTATAACTTA GAAATGGAACAGATGGCCAAGACGGCTAAAGCACTCATGGAAGCTGTGAGCCATGTTCAGGCACCTTTTA CAAGTGCAACACACTTGGAGCACGTGAGGCCGATGTTTAAGTTGGCTTGGACACCTTTTCTGGCTGCATT CAGTGTGGGTCTCCAAGACTGTGATGATACTGAAGTAGCCTCTCTTTGCCTAGAAGGGATAAGATGTGCT ATCAGGATTGCATGCATTTTCAGCATTCAGCTGGAAAGAGATGCATATGTCCAAGCACTAGCCAGATTTA CCTTACTTACAGTGAGTTCTGGTATTACTGAAATGAAACAGAAGAATATCGACACAATAAAAACACTCAT CACGGTGGCTCACACTGATGGAAATTATTTAGGAAATTCGTGGCATGAGATCCTGAAGTGCATCAGTCAG TTAGAGCTGGCACAACTTATAGGAACTGGAGTGAAGCCTCGGTACATTTCTGGGACTGTGAGAGGCAGAG AAGGGTCTCTCACTGGGACAAAAGATCAGGCTCCAGATGAATTTGTTGGTCTAGGGCTAGTTGGAGGAAA TGTGGACTGGAAGCAGATAGCCAGTATTCAGGAGTCTATTGGAGAAACCAGCTCTCAAAGTGTGGTAGTT GCTGTGGATAGAATATTCACCGGATCCACAAGGCTAGATGGAAATGCTATTGTTGATTTTGTCCGTTGGC TATGTGCTGTGTCTATGGATGAATTGCTTTCTACTACACACCCAAGAATGTTTAGTCTACAAAAAATAGT AGAAATATCATACTATAACATGGGAAGAATAAGACTGCAGTGGTCTCGGATTTGGGAAGTTATTGGAGAT CATTTTAATAAGGTTGGTTGCAATCCTAATGAAGATGTAGCCATATTTGCAGTAGACTCTCTGAGGCAGC TATCAATGAAGTTTTTAGAGAAAGGAGAGCTTGCAAACTTCAGATTCCAAAAGGATTTCTTACGACCTTT TGAACATATAATGAAACGAAACAGGTCTCCAACAATTCGAGATATGGTTGTACGGTGTATAGCACAAATG GTTAATTCTCAGGCTGCAAATATTCGTTCAGGATGGAAGAACATTTTCTCAGTATTCCATCTAGCTGCAT CAGACCAAGATGAAAGCATAGTAGAACTTGCATTTCAGACAACAGGGCACATTGTCACTCTTGTATTTGA AAAACACTTTCCGGCCACCATTGATTCTTTCCAGGATGCAGTGAAGTGTTTGTCTGAATTCGCATGTAAT GCAGCTTTCCCAGACACAAGTATGGAAGCAATCCGACTTATTCGCCATTGTGCAAAATATGTGTCTGATA GACCGCAGGCTTTCAAGGAGTACACAAGTGATGACATGAGCGTGGCACCTGAAGACAGGGTGTGGGTGAG AGGGTGGTTCCCGATCCTCTTTGAGTTGTCTTGTATCATCAATAGATGCAAATTAGATGTAAGAACCAGG GGCTTAACAGTAATGTTTGAAATAATGAAAACATATGGCCACACTTATGAAAAACATTGGTGGCAGGACT TATTCAGAATTGTTTTCAGAATCTTTGACAACATGAAATTGCCAGAACAGCAGACAGAGAAAGCTGAATG GATGACAACAACTTGTAACCATGCCCTTTATGCAATATGTGATGTATTCACCCAGTATTTAGAAGTTCTC AGCGATGTACTTTTGGATGATATTTTTGCCCAGCTTTACTGGTGTGTACAACAAGACAATGAGCAGTTAG CACGATCTGGTACAAACTGCTTAGAAAACGTGGTTATTCTGAATGGTGAAAAGTTTACTCTTGAAATCTG GGATAAAACGTGTAACTGCACCCTGGATATCTTCAAAACCACAATTCCTCACGCGCTTCTGACTTGGCGT CCCACCTCTGGCGAGGCCGAACCCCCATCTCCATCAGCTGTGAGTGAAAAGCCGTTGGATGCAATATCAC AAAAATCTGTAGACATACATGATTCTATTCAACCAAGATCTTCAGATAACAGACAGCAAGCACCACTGGT TTCTGTTTCTACTGTGAGTGAAGAAGTTAGCAAAGTGAAATCCACAGCAAAATTCCCAGAACAAAAATTG TTTGCTGCCCTGTTGATTAAATGTGTTGTGCAGCTGGAACTCATCCAGACCATCGACAACATTGTCTTCT TCCCAGCCACAAGCAAGAAGGAGGATGCAGAGAACTTAGCTGCAGCCCAGAGAGATGCAGTAGACTTCGA TGTTCGAGTTGATACTCAAGACCAAGGAATGTACCGCTTTTTAACATCACAACAACTGTTCAAGCTACTG GACTGCTTATTAGAGTCGCATAGATTTGCAAAAGCATTTAATTCCAACAATGAACAGAGGACTGCTCTGT GGAAAGCAGGCTTCAAAGGCAAGTCCAAGCCCAATCTTCTAAAACAGGAGACAAGCAGCCTGGCGTGTGG GCTGCGCATTCTCTTCCGGATGTACATGGACGAGAGCAGAGTCAGTGCCTGGGAAGAAGTCCAGCAGAGG CTTTTAAACGTCTGCAGGGAAGCGCTGAGCTACTTCCTCACTCTAACATCCGAAAGTCACCGGGAAGCCT GGACTAACTTACTGTTACTGTTCCTCACTAAAGTTCTGAAGATCAGTGACAGCAGGTTTAAAGCTCACGC ATCATTCTACTACCCTCTCTTATGTGAAATTATGCAATTTGACTTGATTCCGGAACTGCGTGCTGTTCTT AGAAGATTTTTCCTGCGAATTGGAATAGTATTTCAAATATCACAACCACCTGAACAGGAACTTGGGATAA ACAGGCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225967 representing NM_001102430
Red=Cloning site Green=Tags(s) MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKVETEKQSPPHGEAKAGSGTLPPVKS KTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGRAPDSTTPGKKLIDRIIETICGCFQG PQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARME NQALQEAKQMERERHRQQQHLLQSPVSHHEPESPHLRYLPPQTVDHINQEHEGDLEPQTHDVDKSLQDDT EPENGSDISSAENEQTEADQATAAETLSKNDILYDGDYEEKPLDIVQSIVEEMVNIIVGDMGEGMAISAS TEGNTGTVEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDA FLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPVFRTNEMFINAIKQYLCVALSKNG VSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVV DIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVN PNSQTTLGQEKPSEQEISEVKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIE QGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFS GKDFVSALRLFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQV KNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSTKQNVASEKQRRLLYNL EMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCA IRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQ LELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVV AVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGD HFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQM VNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACN AAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMSVAPEDRVWVRGWFPILFELSCIINRCKLDVRTR GLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVL SDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWR PTSGEAEPPSPSAVSEKPLDAISQKSVDIHDSIQPRSSDNRQQAPLVSVSTVSEEVSKVKSTAKFPEQKL FAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLL DCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEVQQR LLNVCREALSYFLTLTSESHREAWTNLLLLFLTKVLKISDSRFKAHASFYYPLLCEIMQFDLIPELRAVL RRFFLRIGIVFQISQPPEQELGINRQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001102430 |
| ORF Size | 5538 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001102430.1, NP_001095900.1 |
| RefSeq Size | 7042 bp |
| RefSeq ORF | 5541 bp |
| Locus ID | 211673 |
| UniProt ID | G3X9K3 |
| Gene Summary | Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224976 | Arfgef1 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1), (10ug) |
CNY 29,550.00 |
|
| MR225967 | Arfgef1 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1) |
CNY 12,408.00 |
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