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Irs1 (NM_010570) Mouse Tagged ORF Clone
CAT＃: MG226148
- TrueORF®
Irs1 (tGFP-tagged) - Mouse insulin receptor substrate 1 (Irs1), (10ug)
ORF Plasmid: DDK
"NM_010570" in other vectors (4)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | G972R; IRS-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226148 representing NM_010570
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCCCTCCGGATACCGATGGCTTCTCAGACGTGCGCAAGGTGGGCTACCTGCGCAAGCCCAAGA GTATGCATAAGCGCTTTTTCGTGCTGCGGGCGGCCAGCGAGGCCGGGGGCCCAGCGCGCCTGGAGTATTA TGAGAACGAGAAGAAGTGGCGGCACAAGTCGAGCGCCCCCAAACGCTCGATCCCCCTCGAGAGCTGTTTC AACATCAACAAGCGGGCTGACTCCAAGAACAAGCACCTGGTGGCTCTCTACACCCGAGACGAACACTTTG CCATTGCCGCGGATAGCGAGGCTGAGCAAGACAGCTGGTACCAGGCTCTTCTGCAGCTGCATAATCGGGC AAAGGCCCACCATGACGGGGCTGGAGGAGGCTGCGGCGGTAGCTGCAGCGGCAGCTCTGGCGTTGGAGAG GCAGGGGAGGACTTGAGCTATGACACGGGTCCAGGACCCGCGTTCAAGGAGGTCTGGCAGGTTATCCTGA AACCCAAGGGCCTGGGTCAGACAAAGAACCTGATTGGCATCTACCGCCTCTGCCTGACCAGCAAGACCAT CAGCTTTGTGAAGCTGAACTCCGAGGCAGCCGCTGTGGTGCTGCAGCTGATGAATATCAGACGCTGTGGC CACTCAGAGAACTTCTTCTTCATCGAGGTGGGGCGTTCCGCTGTGACAGGGCCTGGCGAGTTCTGGATGC AAGTGGATGACTCCGTGGTGGCCCAGAACATGCATGAGACCATTCTAGAGGCAATGAGGGCCATGAGCGA TGAGTTTCGCCCTCGCAGCAAAAGCCAGTCTTCATCCAGTTGCTCCAACCCCATCAGCGTTCCCCTGCGC AGGCACCATCTCAACAACCCTCCACCCAGCCAGGTGGGACTGACTCGGAGATCTCGAACTGAGAGCATCA CTGCCACCTCCCCTGCCAGTATGGTGGGTGGGAAACCAGGTTCCTTCCGGGTGCGTGCCTCCAGCGATGG CGAAGGCACCATGTCCCGTCCAGCATCAGTGGATGGCAGTCCTGTGAGCCCTAGCACCAACAGGACCCAC GCCCATCGGCATCGAGGCAGCTCCAGGCTGCACCCCCCACTCAACCACAGCCGCTCCATCCCCATGCCTT CTTCTAGATGCTCACCTTCAGCCACCAGCCCAGTGAGTCTGTCATCTAGTAGTACCAGTGGCCATGGCTC CACTTCAGACTGTCTCTTCCCGAGGCGCTCTAGTGCTTCCGTGTCCGGTTCGCCTAGCGATGGCGGTTTC ATCTCTTCTGATGAGTATGGTTCTAGTCCCTGCGATTTCCGAAGTTCCTTCCGCAGTGTCACCCCAGATT CCCTGGGGCACACCCCACCAGCCAGGGGTGAGGAGGAGCTGAGCAATTATATCTGCATGGGTGGCAAGGG AGCCTCCACCTTGGCTGCTCCCAATGGCCACTACATTTTGTCTAGGGGTGGCAACGGCCATCGCTACATC CCAGGTGCTAACTTGGGGACAAGCCCAGCGCTGCCTGGAGATGAAGCCGCGGGTGCAGCAGATCTGGATA ACCGGTTTCGAAAGAGAACTCACTCCGCAGGCACATCTCCTACCATTTCCCATCAGAAGACCCCCTCACA GTCTTCAGTGGCTTCTATTGAGGAATATACAGAGATGATGCCCGCTGCCTACCCACCAGGAGGTGGCAGT GGAGGCCGACTGCCCGGCTACCGGCATTCCGCCTTCGTGCCCACCCACTCCTATCCCGAAGAGGGTCTAG AGATGCACCACTTGGAACGTCGTGGAGGCCACCACCGTCCAGACACCTCCAACCTCCACACTGATGATGG CTATATGCCCATGTCTCCTGGGGTGGCTCCAGTGCCCAGCAACCGCAAAGGAAATGGGGACTATATGCCC ATGAGCCCCAAGAGTGTATCTGCCCCACAGCAGATCATTAACCCCATCAGACGCCACCCACAGAGAGTGG ACCCCAATGGCTACATGATGATGTCACCCAGTGGTAGTTGCTCCCCTGACATTGGAGGTGGGTCCAGCAG CAGTAGCAGCATCAGCGCAGCCCCTTCTGGGAGCAGCTATGGGAAGCCATGGACAAATGGAGTAGGGGGG CACCATACTCATGCCCTTCCTCATGCCAAACCTCCTGTTGAGAGTGGTGGCGGTAAGCTCTTGCCTTGCA CAGGTGACTACATGAACATGTCCCCAGTGGGAGATTCCAACACCAGCAGCCCCTCAGAATGCTACTATGG CCCAGAAGATCCCCAGCACAAGCCGGTCCTCTCTTACTACTCATTGCCAAGGTCCTTTAAGCACACCCAG CGCCCTGGAGAGCCAGAGGAGGGTGCCAGGCACCAGCATCTTCGTCTCTCTTCTAGCTCTGGACGCCTTC GCTATACCGCAACTGCCGAAGATTCCTCCTCTTCTACCAGCAGCGACAGTCTGGGTGGGGGTTACTGTGG GGCTAGGCCAGAGTCAAGCCTCACACATCCCCACCACCATGTCTTGCAGCCCCACCTGCCTCGAAAGGTA GACACAGCTGCACAGACCAACAGCCGCCTGGCTCGACCCACAAGGCTGTCCTTGGGGGATCCCAAGGCAA GCACCTTACCCCGGGTTCGAGAGCAGCAACAGCAGCAGCAGTCTTCCCTGCACCCTCCCGAGCCCAAAAG CCCAGGAGAATATGTGAATATTGAATTCGGGAGCGGCCAGCCTGGCTATTTAGCTGGCCCTGCAACTTCC CGTAGCTCTCCTTCAGTTCGATGTCCACCCCAGCTCCACCCAGCTCCTAGAGAAGAGACTGGCTCGGAAG AGTACATGAACATGGACTTGGGGCCAGGCCGGAGGGCAACCTGGCAGGAGAGTGGTGGAGTTGAGTTGGG CAGAATAGGCCCTGCACCTCCGGGGTCTGCTACGGTTTGCAGGCCAACCCGTTCGGTGCCAAATAGCCGT GGTGACTACATGACCATGCAGATAGGTTGTCCTCGTCAAAGCTATGTGGATACCTCACCAGTGGCCCCAG TCAGCTATGCTGACATGCGGACAGGCATTGCTGCAGAGAAGGCGAGCCTGCCTAGACCCACAGGAGCTGC TCCTCCTCCATCCTCCACAGCCTCTTCTTCTGTTACACCTCAAGGAGCCACCGCTGAGCAAGCTACTCAC TCTTCCTTGCTGGGAGGCCCTCAGGGACCTGGGGGCATGAGTGCATTCACCAGGGTGAACCTCAGTCCCA ACCATAACCAGAGTGCCAAAGTGATTCGCGCAGACACTCAAGGGTGCCGGAGGAGGCATAGCTCTGAGAC CTTCTCAGCACCTACTCGGGCTGGCAATACGGTGCCCTTTGGAGCAGGGGCTGCAGTAGGGGGCAGCGGC GGTGGTGGTGGTGGCGGCAGTGAGGATGTAAAACGCCACAGCTCTGCATCCTTTGAGAATGTGTGGCTGA GACCTGGGGATCTAGGGGGAGTCTCCAAGGAGTCGGCTCCAGTGTGTGGGGCTGCTGGGGGTTTGGAGAA GAGTCTTAACTACATAGACCTGGATTTGGCCAAGGAGCGCTCTCAGGACTGCCCCTCTCAACAGCAGTCC CTACCACCCCCTCCCCCTCACCAGCCCTTAGGCAGCAATGAGGGCAACTCCCCAAGACGCTCCAGTGAGG ATTTAAGCAACTATGCCAGCATCAGCTTCCAGAAGCAGCCAGAGGATCGTCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226148 representing NM_010570
Red=Cloning site Green=Tags(s) MASPPDTDGFSDVRKVGYLRKPKSMHKRFFVLRAASEAGGPARLEYYENEKKWRHKSSAPKRSIPLESCF NINKRADSKNKHLVALYTRDEHFAIAADSEAEQDSWYQALLQLHNRAKAHHDGAGGGCGGSCSGSSGVGE AGEDLSYDTGPGPAFKEVWQVILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCG HSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEFRPRSKSQSSSSCSNPISVPLR RHHLNNPPPSQVGLTRRSRTESITATSPASMVGGKPGSFRVRASSDGEGTMSRPASVDGSPVSPSTNRTH AHRHRGSSRLHPPLNHSRSIPMPSSRCSPSATSPVSLSSSSTSGHGSTSDCLFPRRSSASVSGSPSDGGF ISSDEYGSSPCDFRSSFRSVTPDSLGHTPPARGEEELSNYICMGGKGASTLAAPNGHYILSRGGNGHRYI PGANLGTSPALPGDEAAGAADLDNRFRKRTHSAGTSPTISHQKTPSQSSVASIEEYTEMMPAAYPPGGGS GGRLPGYRHSAFVPTHSYPEEGLEMHHLERRGGHHRPDTSNLHTDDGYMPMSPGVAPVPSNRKGNGDYMP MSPKSVSAPQQIINPIRRHPQRVDPNGYMMMSPSGSCSPDIGGGSSSSSSISAAPSGSSYGKPWTNGVGG HHTHALPHAKPPVESGGGKLLPCTGDYMNMSPVGDSNTSSPSECYYGPEDPQHKPVLSYYSLPRSFKHTQ RPGEPEEGARHQHLRLSSSSGRLRYTATAEDSSSSTSSDSLGGGYCGARPESSLTHPHHHVLQPHLPRKV DTAAQTNSRLARPTRLSLGDPKASTLPRVREQQQQQQSSLHPPEPKSPGEYVNIEFGSGQPGYLAGPATS RSSPSVRCPPQLHPAPREETGSEEYMNMDLGPGRRATWQESGGVELGRIGPAPPGSATVCRPTRSVPNSR GDYMTMQIGCPRQSYVDTSPVAPVSYADMRTGIAAEKASLPRPTGAAPPPSSTASSSVTPQGATAEQATH SSLLGGPQGPGGMSAFTRVNLSPNHNQSAKVIRADTQGCRRRHSSETFSAPTRAGNTVPFGAGAAVGGSG GGGGGGSEDVKRHSSASFENVWLRPGDLGGVSKESAPVCGAAGGLEKSLNYIDLDLAKERSQDCPSQQQS LPPPPPHQPLGSNEGNSPRRSSEDLSNYASISFQKQPEDRQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010570 |
| ORF Size | 3693 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010570.4, NP_034700.2 |
| RefSeq Size | 9163 bp |
| RefSeq ORF | 3696 bp |
| Locus ID | 16367 |
| UniProt ID | P35569 |
| Gene Summary | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224006 | Irs1 (untagged) - Mouse insulin receptor substrate 1 (Irs1), (10ug) |
CNY 15,770.00 |
|
| MR226148 | Irs1 (Myc-DDK-tagged) - Mouse insulin receptor substrate 1 (Irs1) |
CNY 8,568.00 |
|
| MR226148L3 | Lenti ORF clone of Irs1 (Myc-DDK-tagged) - Mouse insulin receptor substrate 1 (Irs1) |
CNY 11,500.00 |
|
| MR226148L4 | Lenti ORF clone of Irs1 (mGFP-tagged) - Mouse insulin receptor substrate 1 (Irs1) |
CNY 11,500.00 |
