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Gprc6a (NM_153071) Mouse Tagged ORF Clone
CAT＃: MG226201
- TrueORF®
Gprc6a (tGFP-tagged) - Mouse G protein-coupled receptor family C group 6 member A (Gprc6a), (10ug)
ORF Plasmid: DDK
"NM_153071" in other vectors (4)
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CNY 11,792.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC124360 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226201 representing NM_153071
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTATTGATTACAGTAGTTACTTGCTTCATGATTATTCTTGATACCTCCCAGTCTTGTCATACCC CAGATGACTTCGTGGCTATCACTTCTCCTGGACATATCATGATTGGTGGCTTGTTTGCCATTCATGAAAA AATGTTGTCCTCAGATGACCATCCCAGACGACCACAAATCCAGAAATGTGCTGGCTTTGAAATATCAGTG TTTCTTCAAACTCTGGCTATGATACACAGCATTGAGATGATCAATAATTCAACACTGTTATCCGGAGTCA AGCTGGGATATGAAATCTATGACACTTGTACCGAAGTCACAGCAGCAATGGCTGCCACTCTGAGGTTCCT CTCAAAATTCAACTGCTCTAGAGAAACTGTGGTCTTTCAGTGTGACTATTCCAGCTACATGCCAAGGGTC AAGGCTGTCATAGGTGCTGGCTACTCTGAAACATCCATCGCGGTCTCAAGGATGCTGAACTTACAGCTCA TGCCACAGGTGAGTTATGAATCCACTGCAGAAATCCTGAGTGACAAGATCCGCTTTCCTTCATTTTTACG GACTGTGCCTAGTGACTTCTACCAAACTAAAGCAATGGCCCACCTGATCCGACAATCGGGATGGAACTGG ATTGGTGCCATAACAACCGATGATGATTATGGAAGACTAGCTCTCAACACATTTGCAATACAGGCTGCTG AAAACAATGTGTGCATTGCCTTCAAAGAGGTTCTGCCAGCCTTCCTCTCAGATAATACCATTGAAGTGAG AATTAACCAGACTCTGGAGAAGATCATTGCAGAAGCCCAGGTTAATGTCATTGTGGTGTTTCTGAGAAAA TTCCATGTTTTCAATCTCTTCACGAAAGCCATTGAAAGGAAAATAAGTAAGATCTGGATTGCTAGTGATA ATTGGTCAACCGCTACCAAGATTATCACCATCCCTAATGTTAAGAAGCTTGGCAAAGTGGTGGGGTTTGC CTTTAGGAGAGGAAATACGTCTTCTTTCCATTCTTTTCTTCAAACTCTGCATATGTACCCCAATGACAAT AACAAACCCCTACATGAATTTGCCATGCTCGTTTCTGCCTGTAAATACATCAAAGACGGTGATTTGAGCC AATGCATATCCAACTATTCTCAGGCAACTTTGACCTATGACACTACCAAGACCATTGAAAACCACTTATT CAAGAGAAATGATTTCCTCTGGCATTATACTGAGCCGGGACTCATTTATAGCATTCAGCTTGCAGTGTTC GCCCTTGGTCATGCCATCCGGGATCTGTGCCAAGCTCGAGACTGCAAGAAACCCAACGCCTTTCAGCCAT GGGAGCTACTTGCCGTGCTGAAAAACGTGACATTCACTGATGGAAGGAATTCATTTCACTTTGATGCCCA CGGGGATTTAAATACTGGTTATGACGTGGTGCTCTGGAAGGAGACCAATGGCCTCATGACTGTCACAAAG ATGGCAGAATATGACCTGCAGCGTGACGTCTTCATCACCACAAACCAAGAAACAAAACATGAATTCAGGA AACTTAAGCAAATTCTATCTAAATGCTCCAAGGAATGCAGCCCTGGTCAAATGAAGAAAGCCACAGGAAG CCAACACAGCTGTTGCTATGAATGTGTGAGCTGCCCAGAAAACCACTACAGTAATGAGACAGATATGGAT CACTGCCTTTTATGCAACAATGAAACCCACTGGGCCCCAGTAAGGAGCACTACCTGCTTTGAAAAGGAAG TGGAGTATCTGGACTGGGATGACTCCTTGGCTCTCCTCCTCATTGCCCTCTCCCTACTTGGAATCGCCTT TGTTCTGGCCATTGGCATAATATTTACAAGAAACCTGAAGACACCTGTCGTGAAATCATCTGGAGGATTA GTGGTCTGCTACGTGATGCTCATCTGCCACGCCCTTAACTTTGCCAGCACAGGCTTTTTTATTGGAGAAC CACAAGACTTTGCGTGCAAGACCAGGCAGACCTTATTTGGTGTGAGCTTTACTCTCTGTGTCTCCTGTAT TTTGACCAAGTCCCTGAAAATTTTGCTAGCTTTCAGTTTTGACCCCAAGCTGACAATGTTCCTGAAGTGC CTTTATAGGCCTGTTCCCATTGTTCTTACCTGCACGGGCATTCAAGTGGTCATTTGCACTCTTTGGCTGG TGCTGGCAGCACCTAGTGTGGAAGAGAATATCTCCTTGCCTAGAGTCATTATCCTGGAATGTGAGGAGGG GTCAGCACTGGCATTTGGTACCATGCTGGGCTACATCACAGTTCTGGCCTTCATTTGCTTTGTATTTGCA TTCAAGGGGAGGAAACTTCCTGAGAATTACAACGAAGCCAAGTTCCTGACCTTTGGGATGCTGATTTACT TCATAGCTTGGATCACATTCATCCCTGTCTATACTACCACATTCGGCAAGTATTTGCCCGCCGTGGAGAT TATAGTCATTCTGATATCCAATTATGGGATTCTCTGCTGTATATTCTTCCCCAAGTGCTACATTATTCTT TGCAAGCAGAAGACTAACACCAAATCAGCCTTCCTCCAGATGGTTTACAACTACTCTGCTCACAGTGTGG ACAGCCTTGCCTTGAGTCATGTTTCCCTGGACTCCACCAGCTACGATACTGCAACAACCAATCAGAGCCC TGGTAACAAGATGACAGCCTGTCAGAACGACAACCATCTTCCTGCACAAGTGCTCCCACACACAGGCACG GCAAAGACTATCAAAGCGTCTAAAACTTTGCGTCAGAAAAGAAGTTCAAGTATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226201 representing NM_153071
Red=Cloning site Green=Tags(s) MALLITVVTCFMIILDTSQSCHTPDDFVAITSPGHIMIGGLFAIHEKMLSSDDHPRRPQIQKCAGFEISV FLQTLAMIHSIEMINNSTLLSGVKLGYEIYDTCTEVTAAMAATLRFLSKFNCSRETVVFQCDYSSYMPRV KAVIGAGYSETSIAVSRMLNLQLMPQVSYESTAEILSDKIRFPSFLRTVPSDFYQTKAMAHLIRQSGWNW IGAITTDDDYGRLALNTFAIQAAENNVCIAFKEVLPAFLSDNTIEVRINQTLEKIIAEAQVNVIVVFLRK FHVFNLFTKAIERKISKIWIASDNWSTATKIITIPNVKKLGKVVGFAFRRGNTSSFHSFLQTLHMYPNDN NKPLHEFAMLVSACKYIKDGDLSQCISNYSQATLTYDTTKTIENHLFKRNDFLWHYTEPGLIYSIQLAVF ALGHAIRDLCQARDCKKPNAFQPWELLAVLKNVTFTDGRNSFHFDAHGDLNTGYDVVLWKETNGLMTVTK MAEYDLQRDVFITTNQETKHEFRKLKQILSKCSKECSPGQMKKATGSQHSCCYECVSCPENHYSNETDMD HCLLCNNETHWAPVRSTTCFEKEVEYLDWDDSLALLLIALSLLGIAFVLAIGIIFTRNLKTPVVKSSGGL VVCYVMLICHALNFASTGFFIGEPQDFACKTRQTLFGVSFTLCVSCILTKSLKILLAFSFDPKLTMFLKC LYRPVPIVLTCTGIQVVICTLWLVLAAPSVEENISLPRVIILECEEGSALAFGTMLGYITVLAFICFVFA FKGRKLPENYNEAKFLTFGMLIYFIAWITFIPVYTTTFGKYLPAVEIIVILISNYGILCCIFFPKCYIIL CKQKTNTKSAFLQMVYNYSAHSVDSLALSHVSLDSTSYDTATTNQSPGNKMTACQNDNHLPAQVLPHTGT AKTIKASKTLRQKRSSSI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153071 |
| ORF Size | 2784 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153071.1, NP_694711.1 |
| RefSeq Size | 2856 bp |
| RefSeq ORF | 2787 bp |
| Locus ID | 210198 |
| UniProt ID | Q8K4Z6 |
| Gene Summary | Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca(2+) and Mg(2+). Activated by extracellular calcium and osteocalcin. Seems to act through a G(q)/G(11) and G(i)-coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinate nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalent ions and its responsiveness to anabolic steroids.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222659 | Gprc6a (untagged) - Mouse G protein-coupled receptor, family C, group 6, member A (Gprc6a), (10ug) |
CNY 11,880.00 |
|
| MR226201 | Gprc6a (Myc-DDK-tagged) - Mouse G protein-coupled receptor, family C, group 6, member A (Gprc6a) |
CNY 10,192.00 |
|
| MR226201L3 | Lenti ORF clone of Gprc6a (Myc-DDK-tagged) - Mouse G protein-coupled receptor, family C, group 6, member A (Gprc6a) |
CNY 11,120.00 |
|
| MR226201L4 | Lenti ORF clone of Gprc6a (mGFP-tagged) - Mouse G protein-coupled receptor, family C, group 6, member A (Gprc6a) |
CNY 11,120.00 |
