United States
Germany
Japan
United Kingdom
China
Lmx1a (NM_033652) Mouse Tagged ORF Clone
CAT＃: MG226375
- TrueORF®
Lmx1a (tGFP-tagged) - Mouse LIM homeobox transcription factor 1 alpha (Lmx1a), (10ug)
ORF Plasmid: DDK
"NM_033652" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,256.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | dr; dreher; Lmx1.1; sst |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226375 representing NM_033652
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGACGGCCTGAAGATGGAGGAGAACTTTCAAAGTGCGATTGAGACCTCGGCATCTTTCTCCTCTT TGCTGGGCAGAGCGGTGAGCCCCAAGTCTGTCTGCGAGGGCTGTCAGCGGGTCATCTCGGACAGGTTTCT GCTGCGGCTCAACGACAGCTTCTGGCACGAGCAATGCGTGCAGTGTGCCTCCTGCAAAGAGCCCCTGGAG ACCACCTGCTTCTACCGGGACAAGAAGCTCTACTGCAAGTACCACTACGAGAAACTGTTTGCTGTCAAAT GTGGGGGCTGCTTCGAGGCCATTGCGCCCAATGAGTTTGTCATGCGTGCCCAGAAGAGCGTATACCACCT GAGCTGCTTCTGCTGCTGCGTCTGTGAGCGACAGCTGCAGAAGGGTGACGAGTTTGTCCTGAAGGAGGGC CAGCTGCTCTGCAAAGGGGACTATGAGAAAGAACGGGAGCTGCTGAGCCTGGTGAGCCCTGCGGCCTCAG ACTCAGGCAAAAGCGATGATGAGGAGAGCCTTTGCAAGTCAGCCCATGGGGCAGGAAAAGGAGCATCAGA GGACGGCAAGGACCATAAGCGACCCAAACGTCCCAGAACCATCCTGACCACTCAGCAGAGGAGAGCATTC AAGGCCTCGTTTGAAGTATCCTCCAAGCCCTGCAGAAAGGTGAGGGAGACTCTGGCTGCGGAGACAGGGC TGAGTGTCCGTGTGGTTCAGGTGTGGTTCCAGAACCAGCGAGCCAAGATGAAGAAGCTGGCCCGGCGACA GCAGCAACAGCAACAGGACCAACAGAACACCCAGAGGCTGACTTCTGCTCAGACAAATGGTAGTGGGAAT GCGGGCATGGAAGGGATCATGAACCCCTATACAACGTTGCCCACCCCACAGCAGCTGCTGGCCATTGAAC AGAGCGTCTACAACTCTGATCCCTTCCGACAGGGTCTCACCCCACCCCAGATGCCTGGAGATCACATGCA CCCCTATGGTGCTGAACCTCTTTTCCATGACTTGGATAGTGATGACACATCTCTCAGTAACCTGGGAGAC TGCTTCCTGGCAACCTCAGAAGCTGGGCCCCTGCAGTCCAGAGTGGGAAACCCCATTGACCATCTGTACT CCATGCAGAATTCCTATTTCACCTCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226375 representing NM_033652
Red=Cloning site Green=Tags(s) MLDGLKMEENFQSAIETSASFSSLLGRAVSPKSVCEGCQRVISDRFLLRLNDSFWHEQCVQCASCKEPLE TTCFYRDKKLYCKYHYEKLFAVKCGGCFEAIAPNEFVMRAQKSVYHLSCFCCCVCERQLQKGDEFVLKEG QLLCKGDYEKERELLSLVSPAASDSGKSDDEESLCKSAHGAGKGASEDGKDHKRPKRPRTILTTQQRRAF KASFEVSSKPCRKVRETLAAETGLSVRVVQVWFQNQRAKMKKLARRQQQQQQDQQNTQRLTSAQTNGSGN AGMEGIMNPYTTLPTPQQLLAIEQSVYNSDPFRQGLTPPQMPGDHMHPYGAEPLFHDLDSDDTSLSNLGD CFLATSEAGPLQSRVGNPIDHLYSMQNSYFTS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033652 |
| ORF Size | 1146 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033652.5, NP_387501.1 |
| RefSeq Size | 3338 bp |
| RefSeq ORF | 1149 bp |
| Locus ID | 110648 |
| UniProt ID | Q9JKU8 |
| MW | 42.9 kDa |
| Gene Summary | Acts as a transcriptional activator by binding to an A/T-rich sequence, the FLAT element, in the insulin gene promoter. Required for development of the roof plate and, in turn, for specification of dorsal cell fates in the CNS and developing vertebrae.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212230 | Lmx1a (untagged) - Mouse LIM homeobox transcription factor 1 alpha (Lmx1a), (10ug) |
CNY 3,990.00 |
|
| MR226375 | Lmx1a (Myc-DDK-tagged) - Mouse LIM homeobox transcription factor 1 alpha (Lmx1a) |
CNY 3,656.00 |
|
| MR226375L3 | Lenti ORF clone of Lmx1a (Myc-DDK-tagged) - Mouse LIM homeobox transcription factor 1 alpha (Lmx1a) |
CNY 4,750.00 |
|
| MR226375L4 | Lenti ORF clone of Lmx1a (mGFP-tagged) - Mouse LIM homeobox transcription factor 1 alpha (Lmx1a) |
CNY 4,750.00 |
