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Ddx4 (NM_010029) Mouse Tagged ORF Clone
CAT＃: MG226456
- TrueORF®
Ddx4 (tGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (Ddx4) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_010029" in other vectors (4)
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CNY 7,700.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AV206478; Mvh; VASA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226456 representing NM_010029
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGATGAAGATTGGGAGGCAGAAATACTCAAACCTCACGTGTCTTCCTATGTTCCTGTATTTGAGA AGGATAAATATTCTTCTGGAGCAAATGGAGACACTTTTAACAGGACTTCAGCTTCATCAGATATTGGCGA GTCTAGTAAAAAAGAGAACACATCTACAACTGGTGGCTTTGGAAGAGGAAAGGGCTTTGGAAACAGAGGT TTTTTAAATAACAAGTTTGAAGAAGGTGATAGCTCTGGTTTCTGGAAAGAGTCTAATAATGACTGTGAAG ATAATCAGACTCGAAGCAGAGGGTTTTCCAAGCGAGGTGGCTGCCAAGATGGAAACGATTCAGAAGCATC AGGCCCGTTCAGAAGAGGGGGAAGAGGCAGTTTCCGAGGCTGCCGTGGAGGATTTGGTCTAGGAAGACCA AATAGTGAATCTGACCAAGATCAGGGGACACAGCGTGGTGGTGGCCTTTTTGGTTCTAGGAAACCAGCAG CAAGTGATTCAGGCAATGGTGACACTTACCAAAGCAGAAGTGGAAGTGGTCGAGGTGGTTACAAAGGTTT AAATGAAGAAGTAGTAACAGGTTCTGGAAAGAATTCTTGGAAGTCAGAAACTGAAGGAGGTGAAAGCAGT GATAGTCAAGGTCCAAAAGTGACATATATACCCCCTCCTCCACCAGAGGATGAGGACTCCATCTTTGCAC ATTATCAGACAGGCATAAACTTTGATAAATATGACACCATACTTGTTGAAGTATCTGGACATGATGCACC ACCGGCAATTTTGACTTTTGAAGAAGCTAATCTCTGTCAGACACTGAATAACAACATTGCTAAAGCTGGC TATACTAAGCTTACTCCTGTGCAGAAGTACAGCATTCCCATTGTATTAGCAGGACGAGATTTGATGGCTT GTGCTCAAACAGGGTCTGGGAAGACTGCAGCTTTTCTCTTGCCTATTTTGGCTCATATGATGCGGGATGG AATAACTGCCAGTCGCTTTAAAGAACTGCAGGAACCAGAGTGTATTATTGTAGCACCAACTCGAGAACTG ATCAACCAAATTTACTTGGAAGCCAGAAAATTTTCTTTTGGGACTTGTGTAAGAGCTGTGGTCATATATG GAGGAACCCAGTTTGGTCATTCAGTTCGACAGATAGTACAAGGGTGTAACATATTGTGTGCTACTCCAGG GAGGCTGATGGACATCATAGGCAAAGAAAAGATTGGCCTCAAACAAGTCAAGTACTTAGTTTTGGATGAA GCTGATCGAATGTTGGATATGGGTTTTGGACCAGAGATGAAGAAACTAATTTCTTGTCCAGGAATGCCAT CAAAGGAACAACGCCAAACCCTTTTATTCAGTGCTACTTTTCCAGAAGAAATCCAGAGGTTGGCTGGGGA CTTTCTAAAGTCCAGTTACTTGTTTGTCGCTGTTGGGCAAGTGGGAGGAGCTTGCAGAGATGTTCAGCAG ACGATCCTTCAAGTTGGCCAGTATTCAAAAAGAGAAAAGCTTGTTGAGATTCTACGAAACATAGGTGATG AAAGAACTATGGTCTTTGTTGAAACCAAGAAAAAAGCCGATTTCATTGCAACTTTTCTTTGTCAAGAAAA AATATCAACTACAAGTATTCATGGTGATCGGGAGCAGAGGGAGAGAGAGCAAGCTCTTGGAGATTTTCGC TGTGGAAAGTGCCCAGTTCTTGTTGCTACTTCAGTGGCTGCCAGAGGGCTTGATATTGAAAATGTTCAAC ATGTTATCAATTTTGACCTTCCTTCTACCATTGATGAGTATGTTCATCGAATTGGACGCACTGGCCGCTG TGGAAATACTGGCAGAGCGATTTCTTTTTTTGATACTGACTCTGATAATCATTTAGCACAGCCTCTAGTT AAAGTACTGTCAGACGCTCAACAGGATGTCCCCGCATGGCTAGAAGAGATTGCCTTCAGTACCTATGTGC CTCCCAGCTTCAGTAGCAGCACAAGAGGGGGGGCTGTGTTTGCATCTGTTGACACGAGGAAGAATTACCA GGGCAAGCACACGTTGAATACAGCGGGGATTTCTTCTTCACAAGCTCCCAATCCAGTTGATGACGAGTCA TGGGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226456 representing NM_010029
Red=Cloning site Green=Tags(s) MGDEDWEAEILKPHVSSYVPVFEKDKYSSGANGDTFNRTSASSDIGESSKKENTSTTGGFGRGKGFGNRG FLNNKFEEGDSSGFWKESNNDCEDNQTRSRGFSKRGGCQDGNDSEASGPFRRGGRGSFRGCRGGFGLGRP NSESDQDQGTQRGGGLFGSRKPAASDSGNGDTYQSRSGSGRGGYKGLNEEVVTGSGKNSWKSETEGGESS DSQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAG YTKLTPVQKYSIPIVLAGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTREL INQIYLEARKFSFGTCVRAVVIYGGTQFGHSVRQIVQGCNILCATPGRLMDIIGKEKIGLKQVKYLVLDE ADRMLDMGFGPEMKKLISCPGMPSKEQRQTLLFSATFPEEIQRLAGDFLKSSYLFVAVGQVGGACRDVQQ TILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFR CGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDTDSDNHLAQPLV KVLSDAQQDVPAWLEEIAFSTYVPPSFSSSTRGGAVFASVDTRKNYQGKHTLNTAGISSSQAPNPVDDES WD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010029 |
| ORF Size | 2106 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010029.2, NP_034159.1 |
| RefSeq Size | 2772 bp |
| RefSeq ORF | 2109 bp |
| Locus ID | 13206 |
| UniProt ID | Q61496 |
| Gene Summary | ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:20439430, PubMed:28633017). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:20439430, PubMed:28633017). Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (PubMed:20439430, PubMed:28633017). Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (PubMed:28633017).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220566 | Ddx4 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (Ddx4), transcript variant 2, (10ug) |
CNY 9,030.00 |
|
| MR226456 | Ddx4 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (Ddx4), transcript variant 2 |
CNY 7,720.00 |
|
| MR226456L3 | Lenti ORF clone of Ddx4 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (Ddx4), transcript variant 2 |
CNY 8,930.00 |
|
| MR226456L4 | Lenti ORF clone of Ddx4 (mGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (Ddx4), transcript variant 2 |
CNY 8,930.00 |
