Nfkbia (NM_010907) Mouse Tagged ORF Clone
CAT#: MG226548
- TrueORF®
Nfkbia (tGFP-tagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (Nfkbia), (10ug)
ORF Plasmid: DDK
"NM_010907" in other vectors (4)
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CNY 5,200.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AI462015; Nfkbi |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226548 representing NM_010907
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCAGCCAGCTGGGCACGGCCAGGACTGGGCCATGGAGGGCCCGCGGGATGGCCTCAAGAAGGAGC GCTTGGTGGACGATCGCCACGACAGCGGCCTGGACTCCATGAAGGACGAGGAGTACGAGCAAATGGTGAA GGAGCTGCGGGAGATCCGCCTGCAGCCGCAGGAGGCGCCGCTGGCCGCCGAGCCCTGGAAGCAGCAGCTC ACGGAGGACGGAGACTCGTTCCTGCACTTGGCAATCATCCACGAAGAGAAGCCGCTGACCATGGAAGTCA TTGGTCAGGTGAAGGGAGACCTGGCCTTCCTCAACTTCCAGAACAACCTGCAGCAGACTCCACTCCACTT GGCTGTGATCACCAACCAGCCAGGAATTGCTGAGGCACTTCTGAAAGCTGGCTGTGATCCTGAGCTCCGA GACTTTCGAGGAAATACCCCTCTACATCTTGCCTGTGAGCAGGGCTGCCTGGCCAGTGTAGCAGTCTTGA CGCAGACCTGCACACCCCAGCATCTCCACTCCGTCCTGCAGGCCACCAACTACAATGGCCACACGTGTCT GCACCTAGCCTCTATCCACGGCTACCTGGCCATCGTGGAGCACTTGGTGACTTTGGGTGCTGATGTCAAC GCTCAGGAGCCCTGCAATGGCCGGACAGCCCTCCACCTTGCGGTGGACCTGCAGAATCCTGACCTGGTTT CGCTCTTGTTGAAATGTGGGGCTGATGTCAACAGGGTAACCTACCAAGGCTACTCCCCCTACCAGCTTAC CTGGGGCCGCCCAAGTACCCGGATACAGCAGCAGCTGGGCCAGCTGACCCTGGAAAATCTCCAGATGCTA CCCGAGAGCGAGGATGAGGAGAGCTATGACACGGAGTCAGAATTCACAGAGGATGAGCTGCCCTATGATG ACTGTGTGTTTGGAGGCCAGCGTCTGACATTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226548 representing NM_010907
Red=Cloning site Green=Tags(s) MFQPAGHGQDWAMEGPRDGLKKERLVDDRHDSGLDSMKDEEYEQMVKELREIRLQPQEAPLAAEPWKQQL TEDGDSFLHLAIIHEEKPLTMEVIGQVKGDLAFLNFQNNLQQTPLHLAVITNQPGIAEALLKAGCDPELR DFRGNTPLHLACEQGCLASVAVLTQTCTPQHLHSVLQATNYNGHTCLHLASIHGYLAIVEHLVTLGADVN AQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQML PESEDEESYDTESEFTEDELPYDDCVFGGQRLTL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010907 |
ORF Size | 942 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010907.2, NP_035037.2 |
RefSeq Size | 1592 bp |
RefSeq ORF | 945 bp |
Locus ID | 18035 |
UniProt ID | Q9Z1E3 |
Gene Summary | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209027 | Nfkbia (untagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (Nfkbia), (10ug) |
CNY 3,600.00 |
|
MR226548 | Nfkbia (Myc-DDK-tagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (Nfkbia) |
CNY 3,600.00 |
|
MR226548L3 | Lenti ORF clone of Nfkbia (Myc-DDK-tagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (Nfkbia) |
CNY 6,000.00 |
|
MR226548L4 | Lenti ORF clone of Nfkbia (mGFP-tagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (Nfkbia) |
CNY 5,890.00 |