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Magi3 (NM_001159354) Mouse Tagged ORF Clone
CAT＃: MG226578
- TrueORF®
Magi3 (tGFP-tagged) - Mouse membrane associated guanylate kinase WW and PDZ domain containing 3 (Magi3) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001159354" in other vectors (4)
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CNY 16,250.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4732496O19Rik; 6530407C02Rik; AA407180; AI120132; mKIAA1634 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226578 representing NM_001159354
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAAGACGTTGAAGAAGAAGAAGCACTGGCTCAGCAAGGTGCAGGAGTGTGCGGTATCCTGGGCCG GGCCCCCGGGCGACTTGGGCGCCGAGATCCGCGGCGGCGCCGAGCGCGGCGAGTTCCCTTACCTGGGGCG GCTCCGCGACGAGGCTGGCGGCGGCGGAGGCACCTGCTGCGTGGTCTCGGGCAAGGCGCCCAGTCCGGGC GATGTGCTGTTGGAGGTGAACGGGACGCCGGTCAGTGGGCTCACCAACCGGGACACTCTGGCTGTCATCC GCCACTTCCGCGAGCCCATCCGTCTGAAGACGGTGAAGCCAGGCAAAGTTATAAATAAAGATTTGCGACA CTACCTGAGTCTTCAGTTTCAGAAAGGATCAATTGACCACAAACTACAGCAAGTCATCAGAGATAATCTC TATTTGAGAACCATCCCATGCACTACAAGGGCTCCCAGGGATGGGGAAGTCCCAGGGGTGGATTATAACT TCATTTCTGTTGAACAATTCAAGGCACTGGAAGAAAGCGGAGCATTGTTAGAAAGTGGAACGTATGATGG AAATTTCTATGGCACACCCAAGCCTCCAGCAGAGCCTAGTCCTTTCCAGCCCGATCCAGTTGATCAGGTC CTCTTTGACAATGAGTTTGACACAGAATCCCAAAGAAAAAGAACCACATCTGTCAGCAAGATGGAAAGGA TGGACAGCTCTCTCCCTGAAGAAGAAGAAGATGAGGACAAAGAAGCTGTTAATGGCAGCGGAAGCATGGA AACTAGAGAGATGCATTCTGAGACATCTGACTGCTGGATGAAGACTGTTCCAAGTTATAACCAAACAAAT AGCTCCATGGACTTTAGAAATTATATGATGAGAGATGAGAATCTGGAACCGCTGCCCAAAAACTGGGAAA TGGCCTACACTGACACAGGAATGATCTACTTCATTGATCATAATACGAAGACAACCACCTGGTTGGATCC TCGTCTCTGCAAGAAAGCCAAAGCCCCTGAAGACTGTGAAGATGGAGAGCTTCCCTATGGCTGGGAGAAA ATAGAGGACCCTCAGTATGGAACATACTACGTTGATCACCTCAACCAGAAAACTCAGTTTGAAAATCCAG TGGAGGAAGCCAAGAGGAAAAAGCAGTTAGGACAGGCTGAAATTCATTCTGCAAAAACAGATGTGGAAAG AGCACACTTTACTCGGGACCCATCCCAACTTAAAGGTGTACTTGTTCGAGCATCGCTGAAAAAAAGCACC ATGGGCTTTGGCTTTACCATTATTGGTGGAGATAGGCCTGATGAATTTCTACAAGTAAAAAACGTGCTCA AAGATGGTCCTGCCGCTCAGGATGGGAAAATTGCACCAGGTGATGTTATTGTAGACATCAATGGCAACTG TGTCCTTGGTCACACCCATGCAGATGTTGTCCAGATGTTTCAACTGGTACCTGTCAACCAGTATGTCAAC CTTACTTTATGCCGTGGTTATCCACTTCCTGATGACAGTGAAGATCCTGTTGTGGACATTGTTGCTGCTA CCCCTGTCATCAATGGACAATCTTTAACAAAGGGAGAGACATGCATGAATACTCAGGATTTTAAACTGGG AGCAATGGTTTTGGATCAGAATGGAAAATCAGGACAAATCTTGGCCAGTGATCGTCTCAATGGTCCATCT GAGTCAAGTGAGCAGAGGGCATCTCTGGCATCATCAGGCAGCTCACAGCCTGAACTAGTGACTATCCCTC TGATTAAGGGCCCCAAAGGCTTTGGGTTTGCAATTGCTGACAGCCCAACTGGACAGAAGGTGAAAATGAT ATTGGATAGCCAGTGGTGTCAAGGCCTTCAAAAAGGGGATATCATCAAAGAAATTTACCATCAGAATGTG CAGAATTTAACACATCTCCAAGTCGTAGAAGTCCTAAAGCAGTTCCCAGTAGGTGCAGATGTTCCATTGC TTATCTTAAGAGGAGGTCCTTGTTCACCAACTAAAACTGCCAAAACGAAAACAGATACAAAGGAAAATTC AGGAAGTTTGGAGACTATAAATGAGCCTATTCCCCAGCCTATGCCTTTTCCGCCCAGCATAATCAGATCA GGATCCCCAAAATTGGATCCTTCTGAGGTCTACCTGAAATCTAAGACTTTATATGAAGATAAACCACCAA ACACCAAAGATCTGGATGTCTTTCTTCGGAAACAAGAATCAGGGTTTGGCTTCAGGGTGCTAGGAGGAGA TGGACCTGACCAGTCTATATATATTGGGGCCATCATTCCCCTGGGAGCAGCTGAAAAAGATGGTAGGCTC CGTGCTGCTGATGAATTGATGTGTATTGATGGGATTCCTGTTAAAGGAAAATCACACAAGCAAGTATTAG ACTTAATGACTACTGCTGCTCGAAATGGACACGTGCTACTAACTGTCAGGAGGAAGATCTTCTATGGAGA GAAACAACCCGAGGACGAAAGCCATCAAGCCTTCTCACAGAACGGATCTCCTCGCCTGAATCGGGCAGAG CTTCCAACCAGGTCTGCCCCACAGGAGGCCTATGATGTCACCTTACAGAGGAAAGAAAATGAAGGGTTTG GTTTTGTCATCCTCACCTCTAAAAGCAAGCCGCCCCCAGGAGTTATTCCTCATAAAATTGGCCGGGTCAT AGACGGAAGTCCAGCTGATCGTTGTGGAGGACTGAAAGTTGGAGATCACATCTCTGCTGTGAATGGGCAG TCCATTGTCGACCTATCCCATGATAATATTGTTCAGCTCATCAAAGATGCTGGAGTCACCGTCACGCTGA CAGTGGTTGCTGAAGAAGAGCATCATGGTCCACCATCAGGAACAAACTCAGCCAGGCAGAGCCCAGCACT ACAGCACAGGCCCATGGGACAAGCACAAGCCAACCACATACCTGGGGACAGAATTGCCCTAGAAGGTGAA ATTGGGAGAGATGTCTGCAGTTCTTACAGACATTCCTGGTCTGACCATAAGCACCTTGCACAGCCTGACA CTGCAGTGATTTCAGTTGTGGGCAGTCGGCACAATCAGAGCCTTGGCTGTTACCCTGTGGAGCTGGAGAG AGGCCCTCGAGGCTTTGGGTTCAGCCTCCGCGGAGGGAAGGAGTACAACATGGGGCTGTTCATCCTGCGC CTGGCCGAGGACGGGCCCGCCATCAAAGACGGCAGGATTCACGTTGGTGACCAGATCGTTGAAATCAATG GGGAACCGACACAAGGCATCACACACACTCGAGCAATTGAGCTCATTCAGGCTGGTGGGAATAAAGTCCT CCTCCTTCTGAGGCCAGGAACTGGCTTGATACCTGACCACGGTGATTGGGATACTAATAGTCCTTCATCT TCCAATGTCATTTATGATGAACAGCCGCCACCTCTCCCATCTTCACATTCTGCTTCCACATTTGAAGAGT CTCATGTGCCAGCAACTGAAGACTCTCTGACTAGAGTTCAGATATGTGAAAAGGCAGAAGAATTAAAGGA CACTGTACAAGAAAAGAAAAGCACTTTAAATGGAAGCCAGCCTGAGATGAAGTATCAGTCTGTCCACAAA ACTATGAGTAAGAAGGATCCACCCAGAGGTTCCGGGCATGGCGAGAAGAGTCGACTAAAAGGAGAAAATG GTGTTACACGGAGAGGTAGATCTGCTAGTCCCAAAAAGTCAGTTAACCGACATTCAGAGGAACATTTGGA AAAGATTCCCAGGCCCCTAAAAAGTGATCCCAAAGAAAAATCTAGAGACCGCTCACTGAGCCCTAGGAAA GGAGAAAGTAAAGGTCGGCTCACCATTAAGGCGGGCTCTGGACAAGATCCCAATAGAAAAGACAGAGGAC GGTCTTCTAGCCCCAAGAAGCAGCAAAAGATAGGAGGCAACAGCCTGTCAAACACTGAAGGCAAATTATC AGAGGCTGGGAGCCGAAGAGCAGCAGGCCACCCTAGAGACAGCACTGAACAGCTCCCAGATGGGAGAGAA AAGTCAGGGGTCAGCAGGAAAGATCTGAAGCAGAGTCAGCCAGGAAAAACCAGAACCAAGTCTCCAGAGA AAAAGAGCAGTAAAGTTGATGAAACATCTCTCCCATCCAAAAAGACTAGCAGCACTGCTGGTAGGGTAGT ATCTGAAAAGGAAAAAGGAAAGAAGCCAACAGCAGGAGAAACAAGTAGAGAAACAGTAGAGCACACCCAG ATCTCAGCAAAGCAGCTCAAGCAGGAAGCTCAAGAGAAGACGGCATTAGGTAACGCAGACGATCACAAAG GCAGAGAATCAGAGGTCACGGACAGATGCAGGGAGCGTGCGGGGTGCACACCTCAAAGTAGCTCTCTAGT CAAGAAAGCACCCATCACTCCAGGACCCTGGAGGGTGCCACGTGCAAATAAAGTCACAGGCACTACTGGC ATGGCTGACAAACAGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226578 representing NM_001159354
Red=Cloning site Green=Tags(s) MSKTLKKKKHWLSKVQECAVSWAGPPGDLGAEIRGGAERGEFPYLGRLRDEAGGGGGTCCVVSGKAPSPG DVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNL YLRTIPCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQV LFDNEFDTESQRKRTTSVSKMERMDSSLPEEEEDEDKEAVNGSGSMETREMHSETSDCWMKTVPSYNQTN SSMDFRNYMMRDENLEPLPKNWEMAYTDTGMIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEK IEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQLGQAEIHSAKTDVERAHFTRDPSQLKGVLVRASLKKST MGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKIAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVN LTLCRGYPLPDDSEDPVVDIVAATPVINGQSLTKGETCMNTQDFKLGAMVLDQNGKSGQILASDRLNGPS ESSEQRASLASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNV QNLTHLQVVEVLKQFPVGADVPLLILRGGPCSPTKTAKTKTDTKENSGSLETINEPIPQPMPFPPSIIRS GSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRL RAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDESHQAFSQNGSPRLNRAE LPTRSAPQEAYDVTLQRKENEGFGFVILTSKSKPPPGVIPHKIGRVIDGSPADRCGGLKVGDHISAVNGQ SIVDLSHDNIVQLIKDAGVTVTLTVVAEEEHHGPPSGTNSARQSPALQHRPMGQAQANHIPGDRIALEGE IGRDVCSSYRHSWSDHKHLAQPDTAVISVVGSRHNQSLGCYPVELERGPRGFGFSLRGGKEYNMGLFILR LAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGDWDTNSPSS SNVIYDEQPPPLPSSHSASTFEESHVPATEDSLTRVQICEKAEELKDTVQEKKSTLNGSQPEMKYQSVHK TMSKKDPPRGSGHGEKSRLKGENGVTRRGRSASPKKSVNRHSEEHLEKIPRPLKSDPKEKSRDRSLSPRK GESKGRLTIKAGSGQDPNRKDRGRSSSPKKQQKIGGNSLSNTEGKLSEAGSRRAAGHPRDSTEQLPDGRE KSGVSRKDLKQSQPGKTRTKSPEKKSSKVDETSLPSKKTSSTAGRVVSEKEKGKKPTAGETSRETVEHTQ ISAKQLKQEAQEKTALGNADDHKGRESEVTDRCRERAGCTPQSSSLVKKAPITPGPWRVPRANKVTGTTG MADKQL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001159354 |
| ORF Size | 4428 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001159354.1, NP_001152826.1 |
| RefSeq Size | 6581 bp |
| RefSeq ORF | 4431 bp |
| Locus ID | 99470 |
| Gene Summary | Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224534 | Magi3 (untagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), transcript variant 1, (10ug) |
CNY 18,910.00 |
|
| MR226578 | Magi3 (Myc-DDK-tagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), transcript variant 1 |
CNY 14,730.00 |
|
| MR226578L3 | Lenti ORF clone of Magi3 (Myc-DDK-tagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), transcript variant 1 |
CNY 16,630.00 |
|
| MR226578L4 | Lenti ORF clone of Magi3 (mGFP-tagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), transcript variant 1 |
CNY 16,630.00 |
