Kcnk2 (NM_010607) Mouse Tagged ORF Clone
CAT#: MG226715
- TrueORF®
Kcnk2 (tGFP-tagged) - Mouse potassium channel subfamily K member 2 (Kcnk2) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_010607" in other vectors (4)
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CNY 3,140.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | A430027H14Rik; AI848635; TREK-1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226715 representing NM_010607
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCCCTGACTTGCTGGATCCCAAGTCTGCTGCTCAGAACTCCAAACCGAGGCTCTCATTCTCCT CAAAACCCACCGTGCTTGCTTCCCGGGTGGAGAGTGACTCGGCCATTAATGTTATGAAATGGAAGACAGT CTCCACGATTTTCCTGGTGGTCGTCCTCTACCTGATCATCGGAGCCACGGTGTTCAAGGCATTGGAGCAG CCTCAGGAGATTTCCCAGAGGACCACCATTGTGATCCAGAAGCAGACCTTCATAGCCCAGCATGCCTGCG TCAACTCCACCGAGCTGGACGAACTCATCCAGCAAATAGTGGCAGCAATAAACGCAGGGATTATCCCCTT AGGAAACAGCTCCAATCAAGTTAGTCACTGGGACCTCGGAAGCTCTTTCTTCTTTGCTGGTACTGTTATC ACAACCATAGGATTTGGAAACATCTCCCCACGAACTGAAGGTGGAAAAATATTCTGCATCATCTATGCCT TGCTGGGAATTCCCCTCTTTGGCTTTCTACTGGCTGGGGTTGGTGATCAGCTAGGAACTATATTTGGAAA AGGAATTGCCAAAGTGGAAGACACATTTATTAAGTGGAATGTTAGTCAGACGAAGATTCGTATCATCTCC ACCATCATCTTCATCCTGTTTGGCTGTGTCCTCTTTGTGGCTCTCCCTGCGGTCATATTCAAGCACATAG AAGGCTGGAGCGCCCTGGACGCTATCTATTTTGTGGTTATCACTCTGACGACCATTGGATTTGGAGACTA CGTGGCAGGTGGATCAGACATTGAATATCTGGACTTCTACAAGCCTGTGGTGTGGTTCTGGATCCTCGTT GGGCTGGCCTACTTTGCAGCTGTTCTGAGCATGATTGGGGACTGGCTACGGGTGATCTCTAAGAAGACGA AGGAAGAGGTGGGAGAGTTCAGAGCGCATGCCGCTGAGTGGACAGCCAATGTCACGGCCGAGTTCAAGGA AACGAGGAGGCGGCTGAGCGTGGAGATCTACGACAAGTTCCAGCGTGCCACATCCGTGAAGCGGAAGCTC TCCGCAGAGCTGGCGGGCAACCACAACCAGGAACTGACTCCGTGTAGGAGGACCCTGTCTGTGAACCACC TGACCAGCGAGAGGGAAGTCCTGCCTCCCTTGCTGAAGGCTGAGAGCATCTATCTGAACGGTCTGACACC ACACTGTGCTGGTGAGGACATAGCTGTCATTGAGAACATGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226715 representing NM_010607
Red=Cloning site Green=Tags(s) MAAPDLLDPKSAAQNSKPRLSFSSKPTVLASRVESDSAINVMKWKTVSTIFLVVVLYLIIGATVFKALEQ PQEISQRTTIVIQKQTFIAQHACVNSTELDELIQQIVAAINAGIIPLGNSSNQVSHWDLGSSFFFAGTVI TTIGFGNISPRTEGGKIFCIIYALLGIPLFGFLLAGVGDQLGTIFGKGIAKVEDTFIKWNVSQTKIRIIS TIIFILFGCVLFVALPAVIFKHIEGWSALDAIYFVVITLTTIGFGDYVAGGSDIEYLDFYKPVVWFWILV GLAYFAAVLSMIGDWLRVISKKTKEEVGEFRAHAAEWTANVTAEFKETRRRLSVEIYDKFQRATSVKRKL SAELAGNHNQELTPCRRTLSVNHLTSEREVLPPLLKAESIYLNGLTPHCAGEDIAVIENMK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010607 |
ORF Size | 1233 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010607.3, NP_034737.2 |
RefSeq Size | 3516 bp |
RefSeq ORF | 1236 bp |
Locus ID | 16526 |
UniProt ID | P97438 |
Gene Summary | Ion channel that contributes to passive transmembrane potassium transport. Reversibly converts between a voltage-insensitive potassium leak channel and a voltage-dependent outward rectifying potassium channel in a phosphorylation-dependent manner. In astrocytes, forms mostly heterodimeric potassium channels with KCNK1, with only a minor proportion of functional channels containing homodimeric KCNK2 (PubMed:24496152). In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1 (PubMed:24496152).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208811 | Kcnk2 (untagged) - Mouse potassium channel, subfamily K, member 2 (Kcnk2), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
MR226715 | Kcnk2 (Myc-DDK-tagged) - Mouse potassium channel, subfamily K, member 2 (Kcnk2), transcript variant 2 |
CNY 3,656.00 |
|
MR226715L3 | Lenti ORF clone of Kcnk2 (Myc-DDK-tagged) - Mouse potassium channel, subfamily K, member 2 (Kcnk2), transcript variant 2 |
CNY 4,750.00 |
|
MR226715L4 | Lenti ORF clone of Kcnk2 (mGFP-tagged) - Mouse potassium channel, subfamily K, member 2 (Kcnk2), transcript variant 2 |
CNY 6,056.00 |