United States
Germany
Japan
United Kingdom
China
Alox5 (NM_009662) Mouse Tagged ORF Clone
CAT＃: MG227106
- TrueORF®
Alox5 (tGFP-tagged) - Mouse arachidonate 5-lipoxygenase (Alox5), (10ug)
ORF Plasmid: DDK
"NM_009662" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 6,370.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5-LO; 5-LOX; 5LO; 5LX; AI850497; F730011J02 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227106 representing NM_009662
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTCCTACACGGTCACCGTGGCCACCGGCAGCCAGTGGTTCGCGGGCACCGACGACTACATCTACC TCAGCCTCATTGGCTCTGCGGGCTGTAGCGAGAAGCATCTGCTGGACAAGGCATTCTACAATGACTTCGA ACGGGGCGCGGTGGACTCCTACGATGTCACCGTGGATGAGGAGCTGGGCGAGATCTACCTAGTCAAAATT GAGAAGCGCAAATACTGGCTCCATGATGACTGGTACCTGAAGTACATCACACTGAAGACACCCCACGGGG ACTACATCGAGTTCCCATGTTACCGCTGGATCACAGGCGAGGGCGAGATTGTCCTGAGGGATGGACGTGC AAAATTGGCCCGAGATGACCAAATTCACATCCTCAAGCAGCACAGACGTAAAGAACTGGAGGCACGGCAA AAACAGTATCGATGGATGGAGTGGAACCCCGGCTTCCCTTTGAGTATTGATGCCAAATGCCACAAGGATC TGCCCCGAGATATCCAGTTTGATAGTGAAAAAGGAGTGGACTTTGTTCTGAACTACTCAAAAGCGATGGA GAACCTGTTCATCAACCGCTTCATGCACATGTTCCAGTCTTCCTGGCACGACTTTGCTGACTTTGAGAAA ATCTTCGTCAAAATCAGCAACACTATATCTGAGCGAGTCAAGAACCACTGGCAGGAAGACCTCATGTTTG GCTACCAGTTCCTGAATGGCTGCAACCCAGTACTCATCAAGCGCTGCACAGCGTTGCCCCCGAAGCTCCC AGTGACCACAGAGATGGTGGAGTGCAGCCTAGAGCGGCAGCTCAGTTTAGAACAGGAAGTACAGGAAGGG AACATTTTCATCGTTGATTACGAGCTACTGGATGGCATCGATGCTAACAAAACTGACCCCTGTACACACC AGTTCCTGGCTGCCCCCATCTGCCTGCTATATAAGAACCTAGCCAACAAGATTGTTCCCATTGCCATCCA GCTCAACCAAACCCCTGGAGAGAGTAACCCAATCTTCCTCCCTACGGATTCAAAGTACGACTGGCTTTTG GCCAAAATCTGGGTGCGTTCCAGTGACTTCCACGTCCATCAAACGATCACCCACCTTCTGCGCACGCATC TGGTGTCTGAGGTGTTTGGTATCGCCATGTACCGCCAGCTGCCTGCTGTGCATCCCCTTTTCAAGCTGCT GGTAGCCCATGTGAGGTTCACCATTGCTATCAACACTAAGGCCCGGGAACAGCTTATCTGCGAGTATGGC CTTTTTGACAAGGCCAATGCCACCGGGGGTGGAGGGCACGTGCAGATGGTGCAGAGGGCTGTCCAGGATC TGACCTATTCCTCCCTGTGTTTCCCGGAGGCCATCAAGGCCCGGGGCATGGACAGCACGGAAGACATCCC CTTCTACTTCTATCGTGATGATGGACTGCTCGTGTGGGAGGCTATCCAGTCGTTCACAATGGAGGTGGTG AGCATCTACTATGAGAACGACCAGGTGGTGGAGGAGGACCAGGAACTGCAGGACTTCGTGAAGGATGTTT ACGTGTACGGCATGCGGGGCAAAAAGGCCTCAGGTTTCCCCAAGTCCATCAAGAGCAGGGAGAAGCTGTC CGAGTACCTGACGGTGGTGATCTTCACGGCCTCTGCCCAGCATGCAGCTGTAAACTTCGGCCAGTATGAC TGGTGCTCCTGGATCCCCAACGCTCCTCCAACTATGCGGGCCCCACCACCCACGGCCAAGGGTGTGGTCA CCATCGAGCAGATCGTGGATACTCTACCAGACCGTGGCCGATCATGTTGGCATCTAGGTGCAGTGTGGGC CTTGAGCCAGTTTCAAGAAAATGAGCTGTTTCTAGGCATGTACCCAGAGGAGCATTTCATTGAGAAGCCA GTGAAGGAAGCCATGATCCGATTCCGCAAGAACCTGGAGGCCATCGTCAGCGTGATCGCCGAGCGCAATA AGAACAAAAAGCTCCCCTACTACTACCTGTCACCAGACAGGATTCCCAACAGCGTAGCCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227106 representing NM_009662
Red=Cloning site Green=Tags(s) MPSYTVTVATGSQWFAGTDDYIYLSLIGSAGCSEKHLLDKAFYNDFERGAVDSYDVTVDEELGEIYLVKI EKRKYWLHDDWYLKYITLKTPHGDYIEFPCYRWITGEGEIVLRDGRAKLARDDQIHILKQHRRKELEARQ KQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWHDFADFEK IFVKISNTISERVKNHWQEDLMFGYQFLNGCNPVLIKRCTALPPKLPVTTEMVECSLERQLSLEQEVQEG NIFIVDYELLDGIDANKTDPCTHQFLAAPICLLYKNLANKIVPIAIQLNQTPGESNPIFLPTDSKYDWLL AKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPLFKLLVAHVRFTIAINTKAREQLICEYG LFDKANATGGGGHVQMVQRAVQDLTYSSLCFPEAIKARGMDSTEDIPFYFYRDDGLLVWEAIQSFTMEVV SIYYENDQVVEEDQELQDFVKDVYVYGMRGKKASGFPKSIKSREKLSEYLTVVIFTASAQHAAVNFGQYD WCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKP VKEAMIRFRKNLEAIVSVIAERNKNKKLPYYYLSPDRIPNSVAI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009662 |
| ORF Size | 2022 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009662.2, NP_033792.1 |
| RefSeq Size | 2827 bp |
| RefSeq ORF | 2025 bp |
| Locus ID | 11689 |
| UniProt ID | P48999 |
| Gene Summary | Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220311 | Alox5 (untagged) - Mouse arachidonate 5-lipoxygenase (Alox5), (10ug) |
CNY 8,650.00 |
|
| MR227106 | Alox5 (Myc-DDK-tagged) - Mouse arachidonate 5-lipoxygenase (Alox5) |
CNY 4,944.00 |
|
| MR227106L1 | Lenti ORF clone of Alox5 (Myc-DDK-tagged) - Mouse arachidonate 5-lipoxygenase (Alox5) |
CNY 7,700.00 |
|
| MR227106L2 | Lenti ORF clone of Alox5 (mGFP-tagged) - Mouse arachidonate 5-lipoxygenase (Alox5) |
CNY 7,344.00 |
|
| MR227106L3 | Lenti ORF clone of Alox5 (Myc-DDK-tagged) - Mouse arachidonate 5-lipoxygenase (Alox5) |
CNY 7,700.00 |
|
| MR227106L4 | Lenti ORF clone of Alox5 (mGFP-tagged) - Mouse arachidonate 5-lipoxygenase (Alox5) |
CNY 7,700.00 |
