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Cntn1 (NM_007727) Mouse Tagged ORF Clone
CAT＃: MG227158
- TrueORF®
Cntn1 (tGFP-tagged) - Mouse contactin 1 (Cntn1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_007727" in other vectors (5)
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CNY 11,210.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW495098; CNTN; F3cam; usl |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227158 representing NM_007727
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAATGCCATTGCTGGTCAGCCATCTCCTGCTAATCTCCTTAACATCTTGTCTAGGAGACTTTACCT GGCACAGAAGATATGGTCATGGAGTGTCTGAGGAGGACAAAGGATTTGGGCCCATTTTTGAAGAGCAGCC AATCAATACCATTTACCCAGAAGAGTCACTGGAAGGAAAAGTGTCACTCAACTGCCGGGCACGCGCCAGC CCATTTCCAGTTTACAAATGGAGAATGAATAACGGGGACGTGGACCTGACAAACGATCGGTACAGTATGG TCGGAGGAAACCTTGTTATCAACAACCCGGACAAACAGAAAGACGCTGGGGTGTACTACTGCCTGGCATC CAACAACTACGGAATGGTCAGGAGCACGGAGGCAACTCTGAGCTTCGGGTATCTCGACCCCTTTCCTCCC GAGGAGCGTCCGGAAGTGAAGGTGAAGGAAGGGAAGGGCATGGTGCTTCTCTGTGATCCTCCCTACCACT TCCCAGATGATCTTAGCTACCGCTGGCTTCTGAATGAATTTCCTGTATTTATCACCATGGATAAGCGAAG ATTTGTGTCTCAGACCAACGGCAATCTCTATATCGCAAATGTCGAGTCTTCTGACAGAGGCAACTACTCC TGCTTTGTGTCCAGTCCTTCTATTACAAAGAGTGTGTTCAGCAAGTTTATTCCCCTCATTCCGATTCCTG AACGAACAACAAAGCCATATCCTGCTGATATTGTGGTGCAGTTCAAGGACATATACACCATGATGGGCCA AAATGTGACTTTAGAGTGTTTCGCTCTAGGGAACCCTGTTCCTGATATCCGGTGGCGGAAGGTGCTAGAA CCAATGCCCAGCACTGCCGAGATAAGCACGTCAGGGGCTGTCCTCAAGATCTTCAACATCCAGCTAGAAG ATGAAGGCCTCTACGAGTGTGAGGCTGAGAACATCCGGGGAAAGGACAAGCACCAGGCAAGAATCTATGT TCAAGCGTTTCCTGAGTGGGTAGAGCATATCAATGACACTGAGGTGGACATAGGCAGTGACCTCTACTGG CCTTGCATAGCCACAGGGAAGCCCATTCCTACCATCAGGTGGCTGAAAAATGGATACTCGTATCACAAAG GGGAACTGAGACTGTATGATGTGACTTTTGAAAATGCCGGGATGTACCAGTGCATAGCAGAAAACGCGTA TGGGTCCATTTATGCCAACGCTGAGCTCAAGATCCTGGCTTTAGCTCCAACTTTTGAAATGAATCCTATG AAGAAAAAGATCTTGGCTGCGAAAGGTGGAAGGGTTGTCATTGAATGTAAACCCAAGGCCGCACCAAAAC CCAAGTTTTCATGGAGCAAAGGGACAGAGTGGCTCGTCAACAGCAGCAGAATACTCATATGGGAAGATGG TAGCTTGGAAATCAATAATATTACAAGAAATGATGGTGGAATCTATACATGCTTTGCAGAAAATAACAGA GGAAAAGCTAATAGCACTGGGACTCTGGTGATCACAAATCCCACACGAATTATTTTGGCCCCAATCAATG CTGACATCACTGTTGGGGAAAATGCCACCATGCAGTGTGCTGCGTCTTTCGATCCTGCCTTGGACCTCAC GTTTGTGTGGTCCTTCAATGGCTACGTGATTGACTTTAACAAGGAAATTACGCATATCCATTACCAGAGG AATTTTATGCTCGACGCCAATGGGGAACTGCTCATCCGAAATGCGCAGCTGAAACACGCAGGCCGGTACA CGTGCACGGCGCAGACAATCGTGGACAATTCCTCGGCTTCCGCTGACCTTGTCGTTCGAGGCCCCCCAGG TCCCCCAGGAGGCCTGAGAATAGAAGACATCAGAGCTACCTCTGTGGCACTGACTTGGAGCCGTGGTTCC GACAATCACAGCCCTATCTCTAAATACACGATCCAGACCAAGACGATTCTCTCAGATGACTGGAAAGATG CGAAGACAGATCCCCCAATCATTGAAGGGAACATGGAGTCAGCTAAAGCAGTGGATTTGATCCCTTGGAT GGAGTATGAATTTCGAGTGGTGGCGACCAATACTTTAGGGACGGGAGAGCCCAGCATACCCTCAAACAGG ATCAAAACAGACGGTGCTGCACCAAATGTGGCTCCCTCAGATGTAGGCGGTGGGGGCGGAACCAACAGAG AGCTGACAATAACGTGGGCGCCTTTGTCGAGAGAGTACCATTACGGTAACAATTTTGGTTACATAGTGGC GTTTAAGCCATTTGATGGAGAAGAGTGGAAGAAAGTGACTGTGACTAACCCAGACACTGGCCGCTACGTC CACAAAGATGAGACCATGACCCCCTCCACCGCGTTTCAAGTCAAAGTGAAAGCCTTCAACAATAAAGGAG ATGGGCCCTACAGCCTCGTAGCTGTCATCAACTCTGCACAAGATGCTCCCAGTGAAGCCCCCACAGAGGT AGGGGTCAAAGTGTTATCATCCTCTGAGATATCTGTCCATTGGAAGCATGTGTTAGAGAAAATTGTGGAA AGCTATCAGATTCGGTACTGGGCTGGGCATGACAAAGAAGCAGCCGCACACAGAGTTCAGGTCACCAGCC AGGAGTACTCAGCCAGACTGGAGAACCTTCTTCCCGACACCCAGTACTTCATTGAAGTGGGAGCCTGCAA CAGTGCTGGATGCGGACCTTCCAGTGATGTGATCGAGACCTTCACCAGAAAAGCCCCTCCTAGCCAACCA CCAAGGATCATCAGTTCGGTGAGATCTGGCTCACGATATATAATCACATGGGATCACGTTGTGGCACTAT CCAATGAATCTACTGTGACAGGCTACAAGATACTCTATAGACCGGATGGCCAACATGACGGCAAGCTGTT CTCAACCCATAAACACTCCATAGAAGTCCCCATCCCCAGAGACGGAGAGTATGTTGTCGAGGTTCGAGCA CACAGCGACGGGGGAGACGGAGTTGTGTCTCAAGTCAAAATTTCAGGCGTGTCCACGCTGTCTTCGAGCC TCCTCAGCTTGCTCCTGCCCTCCCTTGGCTTTCTTGTCTACTCGGAATTC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227158 representing NM_007727
Red=Cloning site Green=Tags(s) MKMPLLVSHLLLISLTSCLGDFTWHRRYGHGVSEEDKGFGPIFEEQPINTIYPEESLEGKVSLNCRARAS PFPVYKWRMNNGDVDLTNDRYSMVGGNLVINNPDKQKDAGVYYCLASNNYGMVRSTEATLSFGYLDPFPP EERPEVKVKEGKGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVESSDRGNYS CFVSSPSITKSVFSKFIPLIPIPERTTKPYPADIVVQFKDIYTMMGQNVTLECFALGNPVPDIRWRKVLE PMPSTAEISTSGAVLKIFNIQLEDEGLYECEAENIRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYW PCIATGKPIPTIRWLKNGYSYHKGELRLYDVTFENAGMYQCIAENAYGSIYANAELKILALAPTFEMNPM KKKILAAKGGRVVIECKPKAAPKPKFSWSKGTEWLVNSSRILIWEDGSLEINNITRNDGGIYTCFAENNR GKANSTGTLVITNPTRIILAPINADITVGENATMQCAASFDPALDLTFVWSFNGYVIDFNKEITHIHYQR NFMLDANGELLIRNAQLKHAGRYTCTAQTIVDNSSASADLVVRGPPGPPGGLRIEDIRATSVALTWSRGS DNHSPISKYTIQTKTILSDDWKDAKTDPPIIEGNMESAKAVDLIPWMEYEFRVVATNTLGTGEPSIPSNR IKTDGAAPNVAPSDVGGGGGTNRELTITWAPLSREYHYGNNFGYIVAFKPFDGEEWKKVTVTNPDTGRYV HKDETMTPSTAFQVKVKAFNNKGDGPYSLVAVINSAQDAPSEAPTEVGVKVLSSSEISVHWKHVLEKIVE SYQIRYWAGHDKEAAAHRVQVTSQEYSARLENLLPDTQYFIEVGACNSAGCGPSSDVIETFTRKAPPSQP PRIISSVRSGSRYIITWDHVVALSNESTVTGYKILYRPDGQHDGKLFSTHKHSIEVPIPRDGEYVVEVRA HSDGGDGVVSQVKISGVSTLSSSLLSLLLPSLGFLVYSEF SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007727 |
| ORF Size | 3060 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007727.2, NP_031753.1 |
| RefSeq Size | 5720 bp |
| RefSeq ORF | 3063 bp |
| Locus ID | 12805 |
| UniProt ID | P12960 |
| Gene Summary | Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201930 | Cntn1 (untagged) - Mouse contactin 1 (Cntn1), transcript variant 2, (10ug) |
CNY 10,664.00 |
|
| MG211462 | Cntn1 (tGFP-tagged) - Mouse contactin 1 (Cntn1) |
CNY 11,210.00 |
|
| MR227158 | Cntn1 (Myc-DDK-tagged) - Mouse contactin 1 (Cntn1), transcript variant 2 |
CNY 10,688.00 |
|
| MR227158L3 | Lenti ORF clone of Cntn1 (Myc-DDK-tagged) - Mouse contactin 1 (Cntn1), transcript variant 2 |
CNY 12,070.00 |
|
| MR227158L4 | Lenti ORF clone of Cntn1 (mGFP-tagged) - Mouse contactin 1 (Cntn1), transcript variant 2 |
CNY 12,070.00 |
