Slc12a2 (NM_009194) Mouse Tagged ORF Clone
CAT#: MG227334
- TrueORF®
Slc12a2 (tGFP-tagged) - Mouse solute carrier family 12 member 2 (Slc12a2), (10ug)
ORF Plasmid: DDK
"NM_009194" in other vectors (4)
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CNY 13,300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 9330166H04Rik; mBSC2; Nkcc1; sy-ns |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227334 representing NM_009194
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGGGCCCGCGGCGCCCTCGTCTGGCGCCCCGCGGCCCGCCCGGGATGGGGACGCGCCGCTGG CTGCCGCCGCCGGGGTGGACCTGCCCGGCACGGCCGTGCCCTCCGGCCAAGAGGATGCTACTACGGCTGG GAGGCAGGCTGGCGGCGGGGTGCGCGGAGAGGGAACCCCAGCGGCGGGCGATGGGCTGGGCAGACCTCTG GGGCCCACCCCCAGCCAGAGCCGCTTCCAGGTGGACCCGGTTTCCGAGAACGCCGGGCGGGCCGCGGCGG CTGCGGCGGCGGCGGCGGCGGCTGCGGCTGCGGCCGGGGCGGCGGGCAAGGAGACCCCCGCGGCCGGGAA AGCCGGCGGTGAGAGCGGCGTGGCCAAGGGCAGCGAGGAAGCCAAGGGCCGCTTCCGCGTGAACTTCGTG GACCCGGCTGCCTCGTCGTCGGCGGACGACAGCCTGTCGGATGCGGCGGGGGTCGGTGGCGACGGGCCCA ACGTGAGCTTCCAGAACGGCGGGGACACGGTGCTGAGCGAGGGCAGCAGCCTGCACTCGGGGGGTGGCAG TGGACACCACCAGCAGTACTACTATGACACCCACACCAACACCTACTACCTGCGCACCTTCGGCCACAAC ACCATGGACGCGGTGCCCAGGATCGACCACTACCGGCACACGGCCGCGCAGCTGGGCGAGAAGCTGCTCC GGCCCAGCCTGGCAGAGCTCCACGATGAGCTGGAAAAGGAACCTTTTGAGGATGGCTTTGCGAATGGAGA AGAAAGTACTCCAACCAGAGATGCTGTGGTCGCATACACTGCCGAGAGTAAAGGAGTTGTAAAGTTTGGC TGGATCAAGGGTGTATTAGTCCGCTGCATGTTAAACATTTGGGGTGTGATGCTCTTTATTAGATTGTCAT GGATCGTGGGTCAAGCTGGGATAGGTCTCTCTGTCGTCGTAATAGCAATGGCCACTGTTGTGACAACTAT CACAGGATTGTCTACTTCAGCAATAGCTACCAATGGGTTCGTGAGAGGAGGAGGAGCGTACTATTTAATC TCTAGAAGTCTAGGGCCGGAATTTGGTGGTGCAATTGGCTTGATCTTTGCTTTTGCCAATGCGGTGGCCG TTGCTATGTATGTTGTCGGATTCGCAGAGACTGTGGTGGAGCTGCTTAAGGAACATTCCATACTTATGAT AGATGAAATCAATGATATCCGAATTATTGGAGCCATTACAGTCGTGATTCTTCTGGGCATCTCAGTGGCT GGAATGGAGTGGGAAGCAAAGGCTCAGATTGTGCTGCTGGTGATCCTTCTCCTGGCCATCGCCGACTTCG TCATAGGGACGTTTATCTCACTGGAGAGCAAGAAGCCCAAGGGTTTCTTTGGCTATAAATCTGAAATATT TAATGAGAACTTTGGGCCGGATTTTCGAGAAGAAGAGACTTTCTTCTCTGTATTTGCCATCTTTTTTCCT GCTGCAACTGGTATTCTAGCTGGAGCAAATATCTCAGGTGATCTTGCAGATCCTCAGTCAGCCATACCCA AAGGAACACTCTTAGCCATTTTGATTACTACAGTGGTTTACATAGGAATTGCAGTGTCTGTGGGTTCGTG TGTTGTTCGGGATGCCACTGGCAATGTTAATGATACCATTACAACAGAGCTGACAAACTGTACTTCTGCA GCCTGCAAACTAAACTTTGACTTCTCTTATTGTGAGAGCAATACTTGTTCTTACGGGCTAATGAACAACT TTCAGGTGATGAGCATGGTGTCAGGATTTGCACCCTTGATCTCTGCAGGGATCTTTTCAGCCACGCTCTC GTCAGCACTAGCATCTCTTGTGAGTGCTCCCAAAATATTTCAGGCTCTATGTAAGGACAATATCTACCCA GCTTTCCAGATGTTTGCTAAAGGTTATGGGAAAAATAATGAACCGCTTCGTGGTTACATCTTAACATTTC TGATTGCACTGGGATTCATCCTGATTGCTGAATTGAACGTTATTGCTCCAATTATCTCTAATTTCTTCCT TGCATCGTATGCCTTAATCAACTTTTCAGTATTCCATGCATCACTTGCAAAATCTCCAGGATGGCGTCCT GCATTCAAATACTACAACATGTGGATCTCCCTTATTGGAGCAATTCTTTGTTGCATAGTGATGTTTGTCA TTAACTGGTGGGCTGCGTTGCTCACATATGTGATAGTCCTTGGGCTGTACATTTATGTTACTTACAAAAA ACCAGATGTGAACTGGGGATCCTCCACACAAGCGCTGACTTACCTCAGTGCACTGCAGCATTCAATCCGT CTTTCTGGGGTGGAGGACCATGTGAAAAACTTCAGACCACAGTGCCTTGTAATGACAGGTTCTCCAAACT CACGGCCTGCTTTACTTCATCTTGTTCATGACTTCACGAAAAATGTTGGTTTAATGATCTGTGGCCATGT GCATATGGGCCCCAGAAGACAAGCTATGAAAGAGATGTCCATTGATCAAGCCAGATATCAGCGATGGCTC ATTAAAAACAAAATGAAGGCTTTCTATGCTCCGGTTCACGCAGATGACTTGCGAGAAGGTGCACAATACT TGATGCAGGCTGCTGGACTTGGTCGTATGAAGCCAAACACACTTGTCCTGGGATTTAAGAAAGATTGGTT ACAAGCCGATATGAGGGATGTGGATATGTATATAAACTTATTTCATGATGCTTTTGACATACAATTTGGA GTTGTGGTTATCCGCCTGAAGGAAGGACTGGATATATCACACCTCCAAGGTCAGGAAGAATTATTATCTT CACAAGAAAAATCACCTGGTACCAAGGATGTGGTAGTAAATGTGGATTACAGTAAAAAGTCAGATCAAGA TACTTGCAAATCATCTGGTGAAAAATCCATTACACAGAAAGATGAGGAAGAGGATGGCAAGACTCCAACT CAGCCACTGTTGAAAAAAGAATCCAAAGGCCCTATTGTGCCTTTAAATGTAGCTGACCAAAAGCTTCTTG AAGCTAGCACACAGTTTCAGAAAAAACAAGGGAAGAATACTATAGATGTCTGGTGGCTTTTTGATGATGG AGGTTTGACATTGTTGATACCCTATCTTCTGACTACCAAGAAAAAGTGGAAAGATTGTAAGATCCGAGTA TTCATTGGTGGAAAAATAAACAGAATAGACCATGACCGGAGAGCGATGGCTACTTTATTGAGTAAATTCC GGATAGACTTCTCTGATATCATGGTCCTTGGAGATATCAACACCAAGCCAAAAAAAGAAAATATTATTGC TTTTGATGACATGATCGAGCCGTACAGACTTCACGAAGATGATAAAGAACAGGACATTGCAGATAAGATG AAAGAAGATGAGCCTTGGCGAATCACAGATAATGAGCTTGAGCTTTATAAGACTAAGACATATCGACAGA TCAGGTTAAATGAATTATTAAAGGAGCATTCAAGCACAGCTAACATTATTGTCATGAGTCTTCCAGTCGC ACGGAAAGGCGCTGTGTCCAGTGCTCTGTATATGGCTTGGTTAGAAGCTCTCTCGAAGGACCTGCCACCA GTCCTCCTGGTTCGTGGGAATCATCAGAGCGTCCTTACCTTCTACTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227334 representing NM_009194
Red=Cloning site Green=Tags(s) MEPGPAAPSSGAPRPARDGDAPLAAAAGVDLPGTAVPSGQEDATTAGRQAGGGVRGEGTPAAGDGLGRPL GPTPSQSRFQVDPVSENAGRAAAAAAAAAAAAAAAGAAGKETPAAGKAGGESGVAKGSEEAKGRFRVNFV DPAASSSADDSLSDAAGVGGDGPNVSFQNGGDTVLSEGSSLHSGGGSGHHQQYYYDTHTNTYYLRTFGHN TMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVAYTAESKGVVKFG WIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLI SRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVA GMEWEAKAQIVLLVILLLAIADFVIGTFISLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFP AATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSA ACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYP AFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRP AFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIR LSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWL IKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFG VVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVNVDYSKKSDQDTCKSSGEKSITQKDEEEDGKTPT QPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRV FIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKM KEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPP VLLVRGNHQSVLTFYS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009194 |
ORF Size | 3618 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009194.3, NP_033220.2 |
RefSeq Size | 6520 bp |
RefSeq ORF | 3621 bp |
Locus ID | 20496 |
UniProt ID | P55012 |
Gene Summary | Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223940 | Slc12a2 (untagged) - Mouse solute carrier family 12, member 2 (Slc12a2), (10ug) |
CNY 15,490.00 |
|
MR227334 | Slc12a2 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 2 (Slc12a2) |
CNY 8,400.00 |
|
MR227334L3 | Lenti ORF clone of Slc12a2 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 2 (Slc12a2) |
CNY 13,970.00 |
|
MR227334L4 | Lenti ORF clone of Slc12a2 (mGFP-tagged) - Mouse solute carrier family 12, member 2 (Slc12a2) |
CNY 13,970.00 |
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