Gsc (NM_010351) Mouse Tagged ORF Clone
CAT#: MG227398
- TrueORF®
Gsc (tGFP-tagged) - Mouse goosecoid homeobox (Gsc), (10ug)
ORF Plasmid: DDK
"NM_010351" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227398 representing NM_010351
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGCCAGCATGTTCAGCATCGACAACATCCTGGCCGCCCGGCCGCGCTGCAAAGACGCGGTGCTCC CGGTGGCGCCCAGCGCCGCGGCTCCGGTGGTCTTCCCGGCTCTACACGGGGACTCGCTCTACGGCGCCGG CGGCGGCACCTCCTCGGACTACGGCGCCTTCTACCCGCGCCCTGTGGCCCCCGGAGGCGCGGGCCTCCCG GCCGCGGTCGGCAGCTCCCGCCTGGGCTACAACAGCTACTTCTACGGGCAGCTGCACGTGCAGGCGGCGC CCGTGGGCCCGGCTTGCTGCGGGGCTGTGCCGCCGCTGGGCGCCCAGCAGTGCTCCTGCGTCCCGACGCC CCCAGGCTACGAGGGCCCCGGTTCTGTACTGGTGTCTCCGGTGCCGCACCAGATGCTGCCCTACATGAAC GTGGGCACGCTGTCGCGCACTGAGCTGCAGCTGCTCAACCAGCTGCACTGTCGGCGGAAGCGGCGGCACC GCACCATCTTCACCGATGAGCAGCTCGAAGCCCTGGAGAACCTCTTCCAGGAGACGAAGTACCCAGACGT GGGCACTCGGGAGCAGCTGGCCAGGAAGGTGCACCTTCGGGAGGAGAAGGTGGAGGTCTGGTTTAAGAAC CGCCGAGCCAAGTGGAGACGACAGAAGCGATCCTCCTCGGAGGAGTCAGAAAACGCCGAGAAGTGGAACA AGACGTCCTCAAAAGCCTCGCCGGAGAAGAGGGAAGAGGAAGGTAAAAGCGATTTGGACTCGGACAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227398 representing NM_010351
Red=Cloning site Green=Tags(s) MPASMFSIDNILAARPRCKDAVLPVAPSAAAPVVFPALHGDSLYGAGGGTSSDYGAFYPRPVAPGGAGLP AAVGSSRLGYNSYFYGQLHVQAAPVGPACCGAVPPLGAQQCSCVPTPPGYEGPGSVLVSPVPHQMLPYMN VGTLSRTELQLLNQLHCRRKRRHRTIFTDEQLEALENLFQETKYPDVGTREQLARKVHLREEKVEVWFKN RRAKWRRQKRSSSEESENAEKWNKTSSKASPEKREEEGKSDLDSDS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010351 |
ORF Size | 768 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010351.1, NP_034481.1 |
RefSeq Size | 1183 bp |
RefSeq ORF | 771 bp |
Locus ID | 14836 |
UniProt ID | Q02591 |
Gene Summary | Regulates chordin (CHRD). May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis (By similarity). In concert with NKX3-2, plays a role in defining the structural components of the middle ear; required for the development of the entire tympanic ring. Goosecoid-expressing regions of the gastrulating mouse egg cylinder have organizer-like activity when transplanted into Xenopus embryos. Probably involved in the regulatory networks that define neural crest cell fate specification and determine mesoderm cell lineages in mammals (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208601 | Gsc (untagged) - Mouse goosecoid homeobox (Gsc), (10ug) |
CNY 2,400.00 |
|
MR227398 | Gsc (Myc-DDK-tagged) - Mouse goosecoid homeobox (Gsc) |
CNY 2,400.00 |
|
MR227398L3 | Lenti ORF clone of Gsc (Myc-DDK-tagged) - Mouse goosecoid homeobox (Gsc) |
CNY 4,750.00 |
|
MR227398L4 | Lenti ORF clone of Gsc (mGFP-tagged) - Mouse goosecoid homeobox (Gsc) |
CNY 4,750.00 |