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Lims1 (NM_201242) Mouse Tagged ORF Clone
CAT＃: MG227467
- TrueORF®
Lims1 (tGFP-tagged) - Mouse LIM and senescent cell antigen-like domains 1 (Lims1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_201242" in other vectors (3)
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CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310016J22Rik; 4921524A02Rik; AI507642; AU021743; AW551584; C430041B13Rik; Lims1l; PINCH; PINCH1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227467 representing NM_201242
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACACTACAGCTTAAGGAGCTTTCACAGTCGGGCCTCTACCGGAGAAGGCGAGAGCGTCCTGAGA GTCTGAGAGTCAATGGGCACGCAGAGGAAGAGCTGAGCAACATGGCCAATGCCTTGGCCAGTGCCACGTG TGAGCGCTGCAAGGGAGGCTTTGCGCCTGCTGAGAAGATCGTGAACAGTAATGGTGAGCTGTACCACGAA CAGTGCTTCGTATGTGCTCAGTGCTTCCAGCAGTTCCCAGAAGGACTCTTTTATGAGTTTGAAGGAAGGA AGTACTGTGAGCATGATTTCCAAATGCTCTTTGCTCCCTGCTGCCACCAATGTGGTGAATTCATCATTGG CAGGGTTATCAAAGCCATGAACAACAGCTGGCATCCTGAGTGCTTCCGATGTGACCTCTGCCAGGAAGTG CTGGCAGACATAGGGTTTGTCAAGAATGCTGGCAGACACCTATGTCGCCCATGTCATAATCGTGAAAAAG CCAGAGGCCTTGGAAAATACATCTGCCAGAAATGCCATGCCATCATTGATGAACAGCCTCTGATCTTCAA GAACGACCCTTACCATCCAGACCACTTCAACTGTGCCAACTGCGGGAAGGAGCTAACTGCTGATGCTCGG GAGCTGAAAGGGGAGCTATACTGTCTGCCATGCCATGATAAGATGGGGGTCCCTATCTGTGGCGCCTGCA GGAGGCCCATTGAAGGGCGAGTAGTGAATGCCATGGGCAAGCAATGGCATGTGGAGCATTTTGTTTGTGC CAAATGTGAAAAACCATTTCTTGGGCATCGCCATTATGAGAGGAAGGGCTTGGCCTACTGTGAAACTCAC TATAATCAGCTGTTTGGTGATGTTTGTTTCCATTGCAACCGTGTCATAGAAGGTGATGTGGTCTCTGCTC TCAACAAGGCCTGGTGTGTGAGCTGCTTTGCCTGCTCCACCTGCAATACCAAACTCACACTCAAGAATAA ATTTGTGGAATTTGACATGAAGCCAGTCTGTAAGAAGTGCTACGAGAAATTTCCTTTGGAGCTGAAGAAA AGACTTAAGAAACTATCTGAGACCTTAGGAAGGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227467 representing NM_201242
Red=Cloning site Green=Tags(s) MTTLQLKELSQSGLYRRRRERPESLRVNGHAEEELSNMANALASATCERCKGGFAPAEKIVNSNGELYHE QCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFIIGRVIKAMNNSWHPECFRCDLCQEV LADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADAR ELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETH YNQLFGDVCFHCNRVIEGDVVSALNKAWCVSCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKK RLKKLSETLGRK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_201242 |
| ORF Size | 1086 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_201242.1, NP_957694.1 |
| RefSeq Size | 4351 bp |
| RefSeq ORF | 1089 bp |
| Locus ID | 110829 |
| Gene Summary | Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR227467 | Lims1 (Myc-DDK-tagged) - Mouse LIM and senescent cell antigen-like domains 1 (Lims1), transcript variant 2 |
CNY 3,610.00 |
|
| MR227467L3 | Lenti ORF clone of Lims1 (Myc-DDK-tagged) - Mouse LIM and senescent cell antigen-like domains 1 (Lims1), transcript variant 2 |
CNY 5,510.00 |
|
| MR227467L4 | Lenti ORF clone of Lims1 (mGFP-tagged) - Mouse LIM and senescent cell antigen-like domains 1 (Lims1), transcript variant 2 |
CNY 5,510.00 |
