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Iqsec1 (NM_001134383) Mouse Tagged ORF Clone
CAT＃: MG227688
- TrueORF®
Iqsec1 (tGFP-tagged) - Mouse IQ motif and Sec7 domain 1 (Iqsec1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001134383" in other vectors (4)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW561907; BC026481; BRAG2; cI-43; D6Ertd349e |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227688 representing NM_001134383
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGTGCCTGCACTGCAACTCAGAGAGGACCCAGTCCTTGCTGGAGCTGGAGCTTGACAGCGGTGTTG AGGGCGAGGCCCCCAGCAGCGAGACTGGCACATCGCTGGACAGCCCCTCAGCCTACCATCAGGGTCCCCT GGTACCTGGTTCCAGCCTGAGTCCAGACCACTACGAGCACACATCAGTGGGCGCCTATGGGCTGTACGCA GGGCCAGGACCACAACAGCGCACACGGAGGCCCCGGCTGCAGCACTCGACCTCAGTCCTGCGCAAGCAGG CTGAGGAGGAGGCCATCAAGCGCTCTCGGTCACTCTCTGAGAGCTATGAGCTCTCTTCAGATCTGCAAGA CAAGCAGGTGGAAATGCTAGAACGCAAGTATGGGGGACGCCTCGTGACCCGCCATGCAGCCCGCACCATC CAGACGGCGTTCCGCCAATACCAGATGAACAAGAACTTTGAGCGCCTGCGCAGCTCCATGTCGGAGAACC GCATGTCACGTCGCATTGTGCTGTCCAACATGAGGATGCAGTTCTCCTTTGAGGGCCCCGAGAAGGTGCA CAGCTCCTACTTTGAGGGAAAGCAGGTCTCTGTGACTAATGATGGCTCCCAGCTGGGGGCCCTGGTGCCA TCAGAATGTGGAGACCTTAGTGACCCAGCCCTCAAGTCTCCAGCCCCCTCCAGTGACTTTGCAGATGCCA TCACGGAACTGGAGGATGCCTTCTCCAGGCAGGTGAAATCCCTGGCTGAATCCATCGATGATGCCCTCAA TTGCCGCAGCCTACACAGTGAGGAGGTACCAGCCTCAGACACGGCCCGGGCTCGGGACACTGAGCCCAAA CCAGGTCTACATGGCATGGACCACCGCAAACTGGATGAGATGACAGCCTCGTATAGTGATGTGACCCTCT ACATTGATGAGGAAGAGCTGTCACCACCGCTTCCACTCTCACAGGCTGGCGATCGGCCCTCCAGCACAGA GTCGGACCTGCGGCTGAGGTCTGGGGGTGCAGCCCAGGACTACTGGGCCTTGGCCCACAAAGAGGACAAG GCTGACACGGACACAAGCTGCCGAAGCACACCATCACTGGAGCGGCCAGAGCCACGGCTGCGGGTGGAGC ACCTGCCCCTGCTTACCATTGAGCCACCCAGTGATAGCTCCGTGGAACTCAGTGACCGCTCAGATCGCAG CTCACTCAAGAGGCAGAGTGCCTATGAGCGCAGCCTCGGTGGGCAGCAGGGCAGCCCCAAGCATGGCCCC CACGGTGGCCCCCCCAAAGGCCTCCCCAGGGAGGAACCTGAATTGCGGCCTCGGCCCCCCAGACCTCTTG AGAGTCACCTAGCCATCAATGGTTCAGCCAACCGGCAGAGCAAATCTGAGTCTGACTACTCAGATGGGGA CAATGACAGCATCAACAGCACCTCCAACTCCAATGACACCATAAACTGCAGCTCTGAGTCCTCATCACGG GACAGCCTGCGGGAACAGACACTCAGCAAGCAGACATACCACAAGGAGACCCGCAACAGCTGGGACTCTC CAGCTTTCAGCAACGATGTCATCCGTAAGAGGCATTACCGAATTGGCCTAAATCTCTTCAACAAGAAGCC TGAGAAGGGCATCCAGTATCTCATTGAGCGCGGCTTCGTGCCCGACACGCCAGTGGGAGTGGCCCACTTC CTGCTGCAGCGCAAGGGCCTCAGCCGCCAGATGATCGGTGAGTTCCTGGGAAACCGGCAGAAGCAGTTCA ACCGTGATGTGCTCGACTGTGTTGTGGACGAGATGGACTTCTCTGCCATGGAGCTAGATGAAGCCCTCCG GAAGTTCCAGGCACACATCCGAGTCCAAGGGGAGGCTCAGAAAGTGGAGCGGCTCATCGAGGCATTCAGC CAGCGGTACTGTGTCTGCAACCCAGGGGTGGTGCGACAGTTCCGGAACCCAGACACCATCTTCATCCTGG CCTTCGCCATCATCCTGCTCAACACAGACATGTACAGCCCCAATGTCAAGCCAGAGCGCAAAATGAAGCT GGAGGACTTTGTCAAGAACCTCCGAGGTGTGGATGACGGTGAAGACATTCCCCGGGAGACGCTAATTGGG ATCTATGAGCGAATCCGTAAGCGAGAACTGAAGACCAACGAGGACCACGTGTCCCAGGTGCAGAAGGTTG AGAAGCTCATCGTGGGCAAGAAGCCGATTGGATCCCTGCATCATGGTCTTGGCTGTGTGCTGTCTCTTCC CCATCGACGACTAGTCTGCTACTGCCGGCTTTTTGAAGTTCCTGACCCCAACAAACCCCAGAAGCTGGGA CTACACCAGCGAGAGATCTTCCTGTTTAATGACCTCCTGGTGGTCACCAAGATCTTCCAGAAGAAGAAGA ACTCGGTGACATACAGCTTCCGGCAGTCCTTCTCCCTCTACGGCATGCAGGTCCTGCTCTTCGAGAACCA GTACTATCCCAATGGCATCCGGCTGACGTCTGCTGTCCCTGGAGCAGACATCAAAGTGCTAATAAACTTC AACGCTCCCAATCCTCAGGACCGGAAGAAGTTCACTGACGACCTGCGGGAGTCTGTCGCTGAAGTGCAAG AGATGGAGAAACACCGAATAGAGTCGGAGCTCGAGAAGCAGAAAGGTGTCGTGCGGCCCAGCATGTCACA GTGCTCCAGCCTCAAAAAGGAGTCAGGCAACGGGACACTGAGCAGGGCTTGCCTGGATGACAGCTATGCC AGTGGCGAGGGCCTCAAGCGCAGCGCCCTCAGCAGCTCACTGAGAGACCTCTCAGAAGCGGGGAAGCGAG GGCGTCGCAGCAGTGCGGGATCGCTAGAGAGCAATGTGGAATTTCAGCCCTTCCAGCCACCACAGCCTCC AGTGCTGTGCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227688 representing NM_001134383
Red=Cloning site Green=Tags(s) MWCLHCNSERTQSLLELELDSGVEGEAPSSETGTSLDSPSAYHQGPLVPGSSLSPDHYEHTSVGAYGLYA GPGPQQRTRRPRLQHSTSVLRKQAEEEAIKRSRSLSESYELSSDLQDKQVEMLERKYGGRLVTRHAARTI QTAFRQYQMNKNFERLRSSMSENRMSRRIVLSNMRMQFSFEGPEKVHSSYFEGKQVSVTNDGSQLGALVP SECGDLSDPALKSPAPSSDFADAITELEDAFSRQVKSLAESIDDALNCRSLHSEEVPASDTARARDTEPK PGLHGMDHRKLDEMTASYSDVTLYIDEEELSPPLPLSQAGDRPSSTESDLRLRSGGAAQDYWALAHKEDK ADTDTSCRSTPSLERPEPRLRVEHLPLLTIEPPSDSSVELSDRSDRSSLKRQSAYERSLGGQQGSPKHGP HGGPPKGLPREEPELRPRPPRPLESHLAINGSANRQSKSESDYSDGDNDSINSTSNSNDTINCSSESSSR DSLREQTLSKQTYHKETRNSWDSPAFSNDVIRKRHYRIGLNLFNKKPEKGIQYLIERGFVPDTPVGVAHF LLQRKGLSRQMIGEFLGNRQKQFNRDVLDCVVDEMDFSAMELDEALRKFQAHIRVQGEAQKVERLIEAFS QRYCVCNPGVVRQFRNPDTIFILAFAIILLNTDMYSPNVKPERKMKLEDFVKNLRGVDDGEDIPRETLIG IYERIRKRELKTNEDHVSQVQKVEKLIVGKKPIGSLHHGLGCVLSLPHRRLVCYCRLFEVPDPNKPQKLG LHQREIFLFNDLLVVTKIFQKKKNSVTYSFRQSFSLYGMQVLLFENQYYPNGIRLTSAVPGADIKVLINF NAPNPQDRKKFTDDLRESVAEVQEMEKHRIESELEKQKGVVRPSMSQCSSLKKESGNGTLSRACLDDSYA SGEGLKRSALSSSLRDLSEAGKRGRRSSAGSLESNVEFQPFQPPQPPVLCS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001134383 |
| ORF Size | 2883 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001134383.1, NP_001127855.1 |
| RefSeq Size | 4526 bp |
| RefSeq ORF | 2886 bp |
| Locus ID | 232227 |
| UniProt ID | Q8R0S2 |
| Gene Summary | Guanine nucleotide exchange factor for ARF1 and ARF6. Guanine nucleotide exchange factor activity is enhanced by lipid binding. Accelerates GTP binding by ARFs of all three classes. Guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222853 | Iqsec1 (untagged) - Mouse IQ motif and Sec7 domain 1 (Iqsec1), transcript variant 1, (10ug) |
CNY 12,260.00 |
|
| MR227688 | Iqsec1 (Myc-DDK-tagged) - Mouse IQ motif and Sec7 domain 1 (Iqsec1), transcript variant 1 |
CNY 9,600.00 |
|
| MR227688L3 | Lenti ORF clone of Iqsec1 (Myc-DDK-tagged) - Mouse IQ motif and Sec7 domain 1 (Iqsec1), transcript variant 1 |
CNY 11,500.00 |
|
| MR227688L4 | Lenti ORF clone of Iqsec1 (mGFP-tagged) - Mouse IQ motif and Sec7 domain 1 (Iqsec1), transcript variant 1 |
CNY 11,500.00 |
