Paip2 (NM_026420) Mouse Tagged ORF Clone

CAT#: MR200634

  • TrueORF®

Paip2 (Myc-DDK-tagged) - Mouse polyadenylate-binding protein-interacting protein 2 (Paip2)

ORF Plasmid: DDK tGFP



  "NM_026420" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 1,200.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2310050K10Rik; AU045972
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR200634 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGATCCAAGTCGCAGCAGTACTAGCCCAAGCATCATCAATGACGATGTGATTATTAACGGTCATT
CTCATGAAGAGGATAATCCATTTGCAGAGTACATGTGGATGGAAAATGAAGAGGAATTCAACAGACAAAT
AGAAGAGGAGTTGTGGGAAGAAGAATTTATTGAACGCTGTTTCCAAGAAATGCTGGAAGAGGAAGAAGAA
CATGAATGGTTTATTCCAGCTCGAGATCTCCCACAAACTATGGACCAAATCCAAGACCAGTTTAATGACC
TTGTTATCAGTGATGGCTCTTCTCTGGAAGATCTTGTGGTCAAGAGCAATCTGAATCCCAATGCAAAGGA
GTTTGTTCCTGGGGTGAAGTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR200634 protein sequence
Red=Cloning site Green=Tags(s)

MKDPSRSSTSPSIINDDVIINGHSHEEDNPFAEYMWMENEEEFNRQIEEELWEEEFIERCFQEMLEEEEE
HEWFIPARDLPQTMDQIQDQFNDLVISDGSSLEDLVVKSNLNPNAKEFVPGVKY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_026420
ORF Size 375 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_026420.3
RefSeq Size 1424 bp
RefSeq ORF 375 bp
Locus ID 67869
UniProt ID Q9D6V8
MW 14.7 kDa
Gene Summary Acts as a repressor in the regulation of translation initiation of poly(A)-containing mRNAs. Its inhibitory activity on translation is mediated via its action on PABPC1. Displaces the interaction of PABPC1 with poly(A) RNA and competes with PAIP1 for binding to PABPC1. Its association with PABPC1 results in disruption of the cytoplasmic poly(A) RNP structure organization (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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