Cep19 (NM_025892) Mouse Tagged ORF Clone
CAT#: MR201297
- TrueORF®
Cep19 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1500031L02 gene (1500031L02Rik)
ORF Plasmid: tGFP
"NM_025892" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1500031L02Rik; AI428934; AL022620 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201297 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATACATTGCCAAGAAATGTGGAGTTAGGTTCCAGCCTCCAGCTGTGATCTTGATTTATGAGAATG AAACCGAAGGAAAGAGCCGCCAGCGTATCATGCCTGTCCGAAACTTTTCAAAGTTCTCAGATTGCACCAG AGCTGCGGAACAGTTAAAGAATAACCCACGGCACAAGAGTTACCTGGAACAGGTGCCCCTGAAGCAGCTG GAGAAGCTGTTTGTTTTTTTGCGAGGTTCCTTGCAGGGGCAGAGCTTGGCAGAAACAATGGAACAGATTC GGCGGGAAACGACCATCGATCCCGAGGAAGACCTGAACAAACTGGACGACAAGGAGCTCGCCAAAAGGAA GAGCATCATGGATGAGCTTTTCGAGAAAAATCAGAAGAGAAAGGACGACCCCACTTTTGTGTACGACGTC GAGGTGGAGTTCCCTCAGGATGAACAGCTGCTGTCCTGCAGCTGGGACACAGCGTCAGTGGATGACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201297 protein sequence
Red=Cloning site Green=Tags(s) MKYIAKKCGVRFQPPAVILIYENETEGKSRQRIMPVRNFSKFSDCTRAAEQLKNNPRHKSYLEQVPLKQL EKLFVFLRGSLQGQSLAETMEQIRRETTIDPEEDLNKLDDKELAKRKSIMDELFEKNQKRKDDPTFVYDV EVEFPQDEQLLSCSWDTASVDDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025892 |
ORF Size | 492 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025892.3 |
RefSeq Size | 1701 bp |
RefSeq ORF | 492 bp |
Locus ID | 66994 |
UniProt ID | Q9CQA8 |
MW | 19.2 kDa |
Gene Summary | Required for ciliation. Recruits the RABL2B GTPase to the ciliary base to initiate ciliation. After specifically capturing the activated GTP-bound RABL2B, the CEP19-RABL2B complex binds intraflagellar transport (IFT) complex B from the large pool pre-docked at the base of the cilium and thus triggers its entry into the cilia. Involved in the early steps in cilia formation by recruiting the ciliary vesicles (CVs) to the distal end of the mother centriole where they fuse to initiate cilium assembly. Involved in microtubule (MT) anchoring at centrosomes.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207603 | Cep19 (untagged) - Mouse RIKEN cDNA 1500031L02 gene (1500031L02Rik), (10ug) |
CNY 3,990.00 |
|
MG201297 | Cep19 (tGFP-tagged) - Mouse RIKEN cDNA 1500031L02 gene (1500031L02Rik) |
CNY 2,850.00 |
|
MR201297L3 | Lenti ORF clone of Cep19 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1500031L02 gene (1500031L02Rik) |
CNY 4,750.00 |
|
MR201297L4 | Lenti ORF clone of Cep19 (mGFP-tagged) - Mouse RIKEN cDNA 1500031L02 gene (1500031L02Rik) |
CNY 4,750.00 |