Gadd45a (NM_007836) Mouse Tagged ORF Clone
CAT#: MR201332
- TrueORF®
Gadd45a (Myc-DDK-tagged) - Mouse growth arrest and DNA-damage-inducible 45 alpha (Gadd45a)
ORF Plasmid: tGFP
"NM_007836" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AA545191; Ddit1; GADD45 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201332 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTTGGAGGAATTCTCGGCTGCAGAGCAGAAGACCGAAAGGATGGACACGGTGGGCGATGCCCTGG AGGAAGTGCTCAGCAAGGCTCGGAGTCAGCGCACCATTACGGTCGGCGTGTACGAGGCTGCCAAGCTGCT CAACGTAGACCCCGATAACGTGGTACTGTGCCTGCTGGCTGCTGACGAAGACGACGACCGGGATGTGGCT CTGCAGATCCATTTCACCCTCATCCGTGCGTTCTGCTGCGAGAACGACATCAACATCCTGCGGGTCAGCA ACCCGGGTCGGCTAGCTGAGCTGCTGCTACTGGAGAACGACGCGGGCCCGGCGGAGAGCGGGGGCGCCGC GCAGACCCCGGACCTGCACTGTGTGCTGGTGACGAACCCACATTCATCACAATGGAAGGATCCTGCCTTA AGTCAACTTATTTGTTTTTGCCGGGAAAGTCGCTACATGGATCAGTGGGTGCCCGTGATTAATCTCCCGG AACGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201332 protein sequence
Red=Cloning site Green=Tags(s) MTLEEFSAAEQKTERMDTVGDALEEVLSKARSQRTITVGVYEAAKLLNVDPDNVVLCLLAADEDDDRDVA LQIHFTLIRAFCCENDINILRVSNPGRLAELLLLENDAGPAESGGAAQTPDLHCVLVTNPHSSQWKDPAL SQLICFCRESRYMDQWVPVINLPER TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_007836 |
ORF Size | 498 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007836.1, NP_031862.1 |
RefSeq Size | 1224 bp |
RefSeq ORF | 498 bp |
Locus ID | 13197 |
UniProt ID | P48316 |
MW | 18.3 kDa |
Gene Summary | Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203784 | Gadd45a (untagged) - Mouse growth arrest and DNA-damage-inducible 45 alpha (Gadd45a), (10ug) |
CNY 1,200.00 |
|
MG201332 | Gadd45a (tGFP-tagged) - Mouse growth arrest and DNA-damage-inducible 45 alpha (Gadd45a) |
CNY 2,850.00 |
|
MR201332L3 | Lenti ORF clone of Gadd45a (Myc-DDK-tagged) - Mouse growth arrest and DNA-damage-inducible 45 alpha (Gadd45a) |
CNY 3,600.00 |
|
MR201332L4 | Lenti ORF clone of Gadd45a (mGFP-tagged) - Mouse growth arrest and DNA-damage-inducible 45 alpha (Gadd45a) |
CNY 3,600.00 |
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